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Tissue-resident macrophages in dental pulp regulate the microenvironment for reparative dentine formation

Research Project

Project/Area Number 17H04400
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionMatsumoto Dental University

Principal Investigator

Nakamura Hiroaki  松本歯科大学, 歯学部, 教授 (50227930)

Co-Investigator(Kenkyū-buntansha) 二宮 禎  日本大学, 歯学部, 准教授 (00360222)
宇田川 信之  松本歯科大学, 歯学部, 教授 (70245801)
細矢 明宏  北海道医療大学, 歯学部, 准教授 (70350824)
堀部 寛治  松本歯科大学, 歯学部, 助教 (70733509)
雪田 聡  静岡大学, 教育学部, 准教授 (80401214)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords修復象牙質 / マクロファージ / 歯髄組織 / Wntシグナル / 歯髄再生療法 / 歯髄 / 組織幹細胞 / デンティンブリッジ
Outline of Final Research Achievements

We investigated the localization of Wnt-related molecules and F4/80-positive macrophages in the process of reparative dentin formation. After directly pulp-capping with MTA cement in the upper first molars of mice, reparative dentin formation was confirmed 14 days after direct pulp capping by micro-CT analysis. Histological analysis revealed that odontoblasts arrayed on the surface of reparative dentin were positive for Wnt3a and Wnt10a.b-catenin was localized in their nucleus. Additionally, F4/80-positive macrophages in the pulp expressed Wnt10a. The localization of b-catenin was found in the nucleus of pulp cells surrounding macrophages. These results suggest that odontoblast- and macrophage-derived Wnt were involved in the reparative odontoblast differentiation.

Academic Significance and Societal Importance of the Research Achievements

本研究は、MTAセメントを取り込んだマクロファージがWnt10aを発現することにより、修復象牙芽細胞分化に深く関わっていることを明らかにしたものである。この現象はこれまでに報告されておらず、今後の歯髄保存療法に多くの示唆を与えるものである。また、Wnt/b-カテニンシグナル以外のマクロファージ由来成長因子が明らかになれば、歯髄保存療法への応用でき、歯科医療の発展に貢献できると考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (4 results)

All 2019 2018

All Presentation (4 results)

  • [Presentation] 修復象牙質形成過程における古典的Wntシグナル組織学的検討2019

    • Author(s)
      原弥革力、堀部寛治、平賀徹、中村浩彰
    • Organizer
      第88回松本歯科大学学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 古典的Wntシグナルによるデンティンブリッジ形成過程の解析2019

    • Author(s)
      原弥革力、堀部寛治、平賀徹、中村浩彰
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] デンティンブリッジ形成過程における古典的Wntシグナルの役割2018

    • Author(s)
      原弥革力、堀部寛治、平賀徹、中村浩彰
    • Organizer
      歯科基礎医学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 修復象牙質形成過程における古典的Wntシグナルの関与2018

    • Author(s)
      原弥革力、堀部寛治、平賀徹、中村浩彰
    • Organizer
      オーラルサイエンス研究会
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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