Uncovering the wild origin of continuous-flowering rose and screening of novel genetic resources

• Project/Area Number: 17H04616
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Horticultural science
• Research Institution: Osaka Institute of Technology
• Principal Investigator: KAWAMURA Koji 大阪工業大学, 工学部, 准教授 (00595613)
• Co-Investigator(Kenkyū-buntansha): 太田垣 駿吾 名古屋大学, 生命農学研究科, 講師 (50597789) ; 松本 省吾 名古屋大学, 生命農学研究科, 教授 (90241489)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 四季咲き性 / 遺伝的多様性 / ゲノム / 自家不和合性 / トランスポゾン / 遺伝資源 / 野生起源 / レトロトランスポゾン / コウシンバラ / ノイバラ / 四季咲き / ゲノム解読 / 園芸遺伝学

Outline of Final Research Achievements

We have investigated the wild origin of the continuous-flowering rose Rosa chinensis 'Old Blush' based on fieldwork in China for wild roses and genetic analyses of their nucleotide sequence diversity and similarity to Rosa chinensis. The results demonstrate hybrid origin of Rosa chinensis and one ancestral species is Rosa chinensis var. spontanea. We have also performed a large-scale screening of genetic resources for continuous-flowering roses and identified a rose cultivar with novel mutation for continuous-flowering characteristics.

Academic Significance and Societal Importance of the Research Achievements

栽培バラは交配育種によって数万品種まで多様化してきたが、その元となった原種はわずか7-8種とされており、遺伝的多様性が低い。栽培バラの遺伝的多様性が低いことは、病原菌耐性や稔性を低下させる要因になっていると考えられるため、新たな遺伝資源を発掘し、今後の品種改良に活かす必要がある。本研究が調べた、中国の野生バラや、新しい四季咲き性の原因変異を持つ栽培品種は、これまで育成されたバラ品種の遺伝的多様性を高めたり、耐病性や新しい形質を付与したりするうえで貴重な遺伝資源となることが期待される。中国の野生バラの多くは絶滅の危機にひんしており、早急な保全対策が必要であり、その社会的意義を科学的に示した。

Research Products

• [Int'l Joint Research] Sichuan University(中国)
• [Int'l Joint Research] INRA(フランス)
• [Int'l Joint Research] University of Hannover(ドイツ)
• [Int'l Joint Research] 四川大学/雲南省農業科学院(中国)
• [Int'l Joint Research] 国立農業研究所(フランス)
• [Int'l Joint Research] IRHS(France)
• [Journal Article] Identification of the genes that regulate ornamental characters using theRosa multifloragenome database (http://rosa.kazusa.or.jp/) (2019)
  • Author(s): Nakamura N.、Hirakawa H.、Sato S.、Otagaki S.、Matsumoto S.、Tabata S.、Tanaka Y.
  • Journal Title: Acta Horticulturae Volume: 1232 Issue: 1232 Pages: 111-118
  • DOI: 10.17660/actahortic.2019.1232.18
  • Peer Reviewed
• [Journal Article] Identification of the genes that regulate ornamental characters using the Rosa multiflora genome database. (2019)
  • Author(s): Nakamura N, Hirakawa H, Sato S, Otagaki S, Matsumoto S, Tabata S, Tanaka Y.
  • Journal Title: Acta Horticulturae Volume: 1232 Pages: 111-118
  • Peer Reviewed
• [Journal Article] A high-quality genome sequence of Rosa chinensis to elucidate ornamental traits (2018)
  • Author(s): Hibrand Saint-Oyant L, Kawamura K, et al
  • Journal Title: Nature Plants Volume: 4 Issue: 7 Pages: 473-484
  • DOI: 10.1038/s41477-018-0166-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome structure of Rosa multiflora, a wild ancestor of cultivated roses (2018)
  • Author(s): N. Nakamura, H. Hirakawa, S. Sato, S. Otagaki, S. Matsumoto, S. Tabata, Y. Tanaka
  • Journal Title: DNA research Volume: 25 Issue: 2 Pages: 113-121
  • DOI: 10.1093/dnares/dsx042
  • Peer Reviewed / Open Access
• [Journal Article] A high-quality genome sequence of Rosa chinensis to elucidate ornamental traits (2018)
  • Author(s): Laurence Hibrand Saint-Oyant, Koji Kawamura (40名中32番目）, et al
  • Journal Title: Nature Plants Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 栽培バラの野生起源を四季咲き性と八重花の原因遺伝子をもとに探る (2020)
  • Author(s): 坂本亮介、上田善弘、王麗、河村耕史
  • Organizer: 日本生態学会第67回全国大会
• [Presentation] ノイバラの自家不和合性を制御するSRNase 遺伝子の探索と配列解析 (2019)
  • Author(s): 坂本亮介, 津川真輝, 落合正樹, 太田垣駿吾, 山田邦夫, 松本省吾, 河村耕史
  • Organizer: 日本園芸学会平成31 年度春季大会
• [Presentation] 栽培バラ四季咲き性の原因遺伝子KSN の遺伝的多様性解析 (2018)
  • Author(s): 坂本亮介, 上田善弘, 王麗, 河村耕史
  • Organizer: 日本園芸学会平成3 0 年度秋季大会
• [Presentation] 栽培バラの祖先種であるノイバラ（Rosa multiflora ）のドラフトゲノム (2017)
  • Author(s): 中村 典子, 平川 英樹, 佐藤 修正,太田垣 駿吾, 松本 省吾, 田畑 哲之, 田中 良和
  • Organizer: 第35回日本植物細胞分子生物学会（さいたま）大会
• [Presentation] バラのトゲ形成に関わる遺伝子の探索と解析 (2017)
  • Author(s): 長和宏, 落合正樹, 福井博一, 太田垣駿吾, 松本省吾
  • Organizer: 平成29年度園芸学会東海支部大会
• [Presentation] Genome structure of Rosa multiflora, an ancestral wild rose of cultivated roses (2017)
  • Author(s): Noriko Nakamura, Hideki Hirakawa, Shusei Sato, Shungo Otagaki, Shogo Matsumoto, Satoshi Tabata, Yoshikazu Tanaka
  • Organizer: VII International Symposium on Rose Research and Cultivation
  • Int'l Joint Research
• [Presentation] The rose genome sequencing initiative (2017)
  • Author(s): Foucher F, Matsumoto S（63名中35番目）, et al.
  • Organizer: VII International Symposium on Rose Research and Cultivation
  • Int'l Joint Research
• [Presentation] Control and selection of blooming seasonality in rose (2017)
  • Author(s): Soufflet-Freslon V, Kawamura K (14名中10番目), et al.
  • Organizer: VII International Symposium on Rose Research and Cultivation
  • Int'l Joint Research
• [Presentation] Isolation of Candidate Genes for Self-incompartibility in Rose (2017)
  • Author(s): Koji Kawamura, Shungo Otagaki, Hibrand-Saint Oyant, Fabrice Foucher, Shogo Matsumoto
  • Organizer: VII International Symposium on Rose Research and Cultivation
  • Int'l Joint Research
• [Presentation] Sequence and copy number variation of Ty1/copia retrotransposon RoRE1 in the rose genome (2017)
  • Author(s): Shungo Otagaki, Takeshi Nakao, Hibrand-Saint Oyant, Fabrice Foucher, Shogo Matsumoto, Koji Kawamura
  • Organizer: VII International Symposium on Rose Research and Cultivation
  • Int'l Joint Research
• [Presentation] バラゲノムにおけるTy1/copia 群レトロトランスポゾンRoRE1 のシークエンス解析およびコピー数測定 (2017)
  • Author(s): 加藤大地・河村耕史・中尾壮志・松本省吾・白武勝裕・太田垣駿吾
  • Organizer: 日本園芸学会 平成29年度秋季大会