• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of pathogenicity and establishment of detection and control methods for viruses detected in vegetables exporting from Southeast Asia to Japan

Research Project

Project/Area Number 17H04617
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Plant protection science
Research InstitutionUtsunomiya University

Principal Investigator

Natsuaki Tomohide  宇都宮大学, 農学部, 教授 (10134264)

Co-Investigator(Kenkyū-buntansha) 園田 昌司  宇都宮大学, 農学部, 教授 (00325127)
煉谷 裕太朗  宇都宮大学, 農学部, 助教 (30773551)
山根 健治  宇都宮大学, 農学部, 教授 (60240066)
西川 尚志  宇都宮大学, 農学部, 准教授 (60361614)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords東南アジア / 植物ウイルス / 遺伝子解析 / LAMP法 / タバココナジラミ / ベゴモウイルス / クリニウイルス / 新ウイルス病 / 次世代シーケンサ / 次世代シーケンサー
Outline of Final Research Achievements

Eggplants, cucumbers, melons, etc. are exported to Japan from Southeast Asian countries. These vegetables have been infected by tropical viral diseases and shown severe symptoms, resulting big yield loss, and Japanese companies have been also damaged. In this study, we focused on viral diseases of vegetables in Southeast Asia, detected several pathogenic viruses with confirming their pathogenicity by infectious clones, established rapid and simple detection method by LAMP method, analyzed pesticide resistance genes of virus vector insects, and examined the barriers for protecting plants from insect vectors.

Academic Significance and Societal Importance of the Research Achievements

新型コロナウイルスはヒトの生命を危険にさらし、爆発的に伝搬して経済的に大被害を及ぼしている。同様に、植物ウイルスは農作物に大被害を引き起こし、ヒトの生活に間接的に影響して経済的損失を引き起こしている。本研究では東南アジアで栽培されて日本に輸出する野菜類の新しい病原ウイルスの遺伝子を解析し、新型コロナウイルスの迅速検出にも用いられたLAMP法を農家圃場でも利用できるように2018年に改良した。また、ウイルス媒介昆虫の防除法を検討した。このような成果は、現地の農業だけでなく今後のわが国への新ウイルス侵入時に利用でき、国際協力の面でも極めて有意義である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (17 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] ガジャマダ大学/ウダヤナ大学/ボゴール農科大学(インドネシア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カセサート大学(タイ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ガジャマダ大学(インドネシア)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Complete genome sequence of a pepper yellow leaf curl Indonesia virus isolated from tomato in Bali,2020

    • Author(s)
      Yutaro Neriya, Runa Izumi, Fariha Wilisiani, Sedyo Hartono, G.N. Alit Susanta Wirya, Hisashi Nishigawa, Tomohide Natsuakia
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 25 Pages: 1-2

    • DOI

      10.1128/mra.00486-20

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Complete Genome Sequence of Tomato Leaf Curl New Delhi Virus from Luffa in Indonesia2019

    • Author(s)
      Wilisiani Fariha、Neriya Yutaro、Tagami Mai、Kaneko Misaki、Hartono Sedyo、Nishigawa Hisashi、Natsuaki Tomohide
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 15 Pages: 1-2

    • DOI

      10.1128/mra.01605-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a LAMP assay with a portable device for real-time detection of begomoviruses under field conditions2019

    • Author(s)
      Wilisiani Fariha、Tomiyama Aika、Katoh Hiroshi、Hartono Sedyo、Neriya Yutaro、Nishigawa Hisashi、Natsuaki Tomohide
    • Journal Title

      Journal of Virological Methods

      Volume: 265 Pages: 71-76

    • DOI

      10.1016/j.jviromet.2018.10.005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The p27 open reading frame of tomato infectious chlorosis virus encodes a suppressor of RNA silencing2019

    • Author(s)
      Mashiko Takaaki、Wang Wei-Qin、Hartono Sedyo、Suastica Gede、Neriya Yutaro、Nishigawa Hisashi、Natsuaki Tomohide
    • Journal Title

      Journal of General Plant Pathology

      Volume: 85 Issue: 4 Pages: 301-305

    • DOI

      10.1007/s10327-019-00850-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] New recombinant of Tomato leaf curl New Delhi virus infecting melon in Indonesia2019

    • Author(s)
      Wilisiani Fariha、Mashiko Takaaki、Wang Wei-Qin、Suzuki Tomohiro、Hartono Sedyo、Neriya Yutaro、Nishigawa Hisashi、Natsuaki Tomohide
    • Journal Title

      Journal of General Plant Pathology

      Volume: 85 Issue: 4 Pages: 306-310

    • DOI

      10.1007/s10327-019-00849-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] First report of the complete genomic sequences from Indonesian isolates of bamboo mosaic virus and detection of genomic recombination events2018

    • Author(s)
      Abe Satomi、Neriya Yutaro、Noguchi Kengo、Hartono Sedyo、Sulandari Sri、Somowiyarjo Susamto、Ali Asad、Nishigawa Hisashi、Natsuaki Tomohide
    • Journal Title

      Journal of General Plant Pathology

      Volume: 85 Issue: 2 Pages: 158-161

    • DOI

      10.1007/s10327-018-0830-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] タイのナス科作物から検出された Begomovirus 属ウイルスの解析2019

    • Author(s)
      冨士川 陽・Fariha Wilisiani・煉谷裕太朗・Kanapol Jutamanee・西川尚志・山根健治・夏秋知英
    • Organizer
      令和元年度日本植物病理学会関東部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] "Vaccines" against plant diseases and their application2018

    • Author(s)
      Tomohide Natsuaki
    • Organizer
      First International Congress of Biological Control
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] トマト退緑ウイルスの感染性クローンの構築2018

    • Author(s)
      益子嵩章 ・王 蔚芹 ・煉谷裕太朗 ・西川尚志 ・夏秋知英
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 携帯増幅装置を用いたLoop-mediated isothermal amplification (LAMP) 法によるベゴモウイルス現場即時検査2018

    • Author(s)
      Fariha Wilisiani・冨山愛佳・加藤 寛・金子海咲・田上真衣・Sedyo Hartono・煉谷裕太朗・西川尚志・夏秋知英
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] インドネシアのトカドヘチマから分離されたBegomovirus属ウイルスの全塩基配列決定2018

    • Author(s)
      金子海咲・田上真衣・Fariha Wilisiani・冨山愛佳・Sedyo Hartono・煉谷裕太朗・西川尚志・夏秋知英
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel mixed infection of two begomoviruses on melon plants in Indonesia2018

    • Author(s)
      Wilisiani, F., Mashiko, T., Wang, W-Q., Hartono, S., Suzuki, T., Neriya, Y., Nishigawa, H., Natsuaki, T.
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] New recombinant of Tomato leaf curl New Delhi virus infecting melon in Indonesia2017

    • Author(s)
      Wilisiani, F., Mashiko, T., Wang, W-Q., Hartono, S., Suzuki, T., Nishigawa, H., Natsuaki, T.
    • Organizer
      The Asian Conference on Plant Pathology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA silencing suppressor of Tomato infectious chlorosis virus.2017

    • Author(s)
      Mashiko, T., Wang ,H-Q., Hartono, S., Suastica, G., Neriya, Y., Nishigawa, H. and Natsuaki, T.
    • Organizer
      The Asian Conference on Plant Pathology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi