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Factors associated with occurrence of novel biotype of Oryctes rhinoceros resistant against nudiviruses

Research Project

Project/Area Number 17H04622
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Forest science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Nakai Madoka  東京農工大学, (連合)農学研究科(研究院), 教授 (60302907)

Co-Investigator(Kenkyū-buntansha) 浅野 眞一郎  北海道大学, 農学研究院, 准教授 (60222585)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywordsタイワンカブトムシ / サイカブト / ヌディウイルス / ヤシ / 太平洋州 / 天敵ウイルス / ハプロタイプ / 抵抗性 / サイカブトムシ / Nudivirus / 昆虫ウイルス / バイオタイプ / ヤシ類 / 共生微生物
Outline of Final Research Achievements

Coconut Rhinoceros Beetle (Oryctes rhinoceros) is a pest of coconut palms and oil palms. Natural enemy of this species, nudivirus, was used to control this species in the 1970s and 1980s, and succeeded in reducing damage to palms in the South Pacific including Fiji. However, in 2007, it was reported that a haplotype (a strain of the same species with a different genotype), in which the virus is ineffective, invaded Guam (USA), and since then this G-type has been expanding its distribution to Palau, Hawaii, Solomon Islands, Papua New Guinea, and other countries. In this study, we isolated natural enemy viruses from Palau populations in order to search for virus strains that could be used to control the G-type.

Academic Significance and Societal Importance of the Research Achievements

パラオのソンソロ州には、島民が天水のみで生活している離島が多数あり、これらの島々では、ココヤシが枯死することは、生命の危機を意味するという。タイワンカブトムシの分布拡大は、太平洋州の住民にとって死活問題と受け止められており、天敵を用いた防除の必要性が叫ばれている。本研究では、パラオのフェロモントラップで採集した成虫が病原力を持ったウイルスに感染していること、しかし、その病原力が従来の生物的防除で使用されている分離株に比べて低いことを示した。さらに、これら成虫の70%近くからウイルスDNAが検出されることから、亜致死感染(致死を引き起こさない感染)成虫同士がウイルスを伝播している可能性を示した。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (29 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (14 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Remarks (2 results) Funded Workshop (1 results)

  • [Int'l Joint Research] グアム大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] パラオコミュニティカレッジ(パラオ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] AgResearch(ニュージーランド)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] グアム大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] パラオコミュニティカレッジ(パラオ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] AgResearch(ニュージーランド)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Guam(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] AgResaerch(ニュージーランド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Palau Community College(パラオ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Ministry of Agriculture and Livestock(ソロモン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Ag Research(New Zealand)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Guam(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Pacific Academic Institute for Research/Palau Community College(Palau)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Institute for Biological Control, JKI(Germany)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] ICTV Virus Taxonomy Profile: Nudiviridae2020

    • Author(s)
      Robert L. Harrison, Elisabeth A. Herniou, Annie Bezier, Johannes A. Jehle, John P. Burand, David A. Theilmann, Peter J. Krell, Monique M. van Oers, Madoka Nakai and ICTV Report Consortium
    • Journal Title

      Journal of General Virology

      Volume: 101 Issue: 1 Pages: 3-4

    • DOI

      10.1099/jgv.0.001381

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] パラオ産タイワンカブトムシからPCRで検出されたヌディウイルスは顕性感染だった2021

    • Author(s)
      田中俊祐・新井大・Christpher Kitalong・Sean Marshall・井上真紀・仲井まどか
    • Organizer
      日本応用動物昆虫学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Overt nudivirus infections confirmed from CRB-G adults in Palau: Virus isolation and characterization of nudivirus2020

    • Author(s)
      Shunsuke Tanaka, Hiroshi Arai, Christpher Kitalong, Sean Marshall, Madoka Nakai
    • Organizer
      CRB-G Action Group Webinar
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Oryctes rhinoceros nudivirusパラオ株のゲノム解析および病理学的特性の解明2020

    • Author(s)
      田中俊祐、井上真紀、仲井まどか
    • Organizer
      第64回日本応用動物昆虫学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Biotype and nudivirus prevalence of Oryctes rhinoceros in Palau2019

    • Author(s)
      Tanaka, S., Kitalong, C., Inoue, N.M., Nakai, M.
    • Organizer
      Society for invertebrate pathology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 太平洋地域に出現したタイワンカブトムシ 新規バイオタイプのウイルス抵抗性要因の探索2018

    • Author(s)
      田中俊祐, 新井大, 仲井まどか
    • Organizer
      2018(平成30)年度 海外学術調査フォーラムプログラム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Oryctes rhinoceros in Japan and its biotype2018

    • Author(s)
      Madoka Nakai
    • Organizer
      51th annual meeting of Society for Invertebrate Pathology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] CRB damage and resistance assessment in the Palau Archipelago2018

    • Author(s)
      Christopher Kitalong, Justin Omak Ramarui, Jason Ngiramengior, Balang Skey, Nelson Masang, Shizu Watanabe, Michael Melzer, Madoka Nakai, Joel Miles
    • Organizer
      51th annual meeting of Society for Invertebrate Pathology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 日本に生息するタイワンカブトムシのバイオタイプとウイルス有病率の調査2018

    • Author(s)
      田中 俊祐 新井 大 井上 真紀 仲井 まどか
    • Organizer
      第13回昆虫病理研究会シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 第62回日本応用動物昆虫学会  筑波大学 2019年3月26日 パラオのタイワンカブトムシのバイオタイプとOrNV有病率の調査2018

    • Author(s)
      田中俊祐, Christopher Kitalong, 井上真紀, 仲井まどか
    • Organizer
      第62回日本応用動物昆虫学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Oryctes rhinoceros in Japan and its biotype2017

    • Author(s)
      Madoka Nakai
    • Organizer
      16th Pacific Entomology Conference featuring the Hawaii Botanical Forum
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ecology of insect viruses: how viruses adapt to their insect host population2017

    • Author(s)
      Madoka Nakai
    • Organizer
      50th of Annual Meeting and Golden Jubilee Celebration of the Society for Invertebrate Pathology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 投稿中の論文のプレプリントに関するURLを記載します

    • URL

      https://www.researchsquare.com/article/rs-379795/v1

    • Related Report
      2020 Annual Research Report
  • [Remarks] 国際研究集会のお知らせ(太平洋地域に出現したタイワンカブトムシ新規バイオタイプの現状と対策)

    • URL

      https://www.tuat.ac.jp/event/general/20171002_01.html

    • Related Report
      2017 Annual Research Report
  • [Funded Workshop] 太平洋地域に出現したタイワンカブトムシ新規バイオタイプの現状と対策2017

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-05-23  

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