Epidemiological and genetic studies on vector-borne parasites and vectors in domesticated animals and chickens in Myanmar

• Project/Area Number: 17H04638
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: KATAKURA Ken 北海道大学, 獣医学研究院, 名誉教授 (10130155)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 節足動物媒介性寄生虫 / 庭先飼育鶏 / 系統解析 / ミャンマー / マラリア原虫 / タイレリア / ロイコチトゾーン / マダニ / ベクター / アジアゾウ / 系統関係 / マイクロビオーム / ミトゲノム / ヤギ / ニワトリ / 住血原虫

Outline of Final Research Achievements

We collected blood from cattle, water buffaloes, goats, and village chickens in Myanmar to investigate haemosporidian parasites. Blood DNA was subjected to identification of parasite species and determination of genetic variation of the parasites for the first time. As a result, it was found that parasites are widely distributed in Myanmar. We also found that Myanmar parasite strains are very close to those reported in neighboring countries Thailand, Malaysia, and China. As these parasites are transmitted by ticks and blood-sucking insects such as mosquitoes and black flies, further research and countermeasures, including investigation of vectors, are necessary.

Academic Significance and Societal Importance of the Research Achievements

ミャンマーは動物や植物資源がとても豊かな国です。また、インド亜大陸と東アジアを結ぶ地理的にも重要な地域です。獣医学的視野にたって、最新の技術を使って家畜や家禽の寄生虫を調査することは、ミャンマーにおける家畜の健康管理、畜産の振興そして食の安全を考えるうえでとても有意義なことでした。この調査研究を通して、ミャンマー獣医科学大学や畜産獣医局との研究交流を継続することができました。ミャンマー側研究者の育成や日本側若手研究者の国際経験を積むことにも貢献することができました。

Research Products

• [Int'l Joint Research] University of Veterinary Science/LBVD/Myanmar Veterinary Association(ミャンマー)
• [Int'l Joint Research] University of Veterinary Science/LBVD(Myanmar)
• [Journal Article] Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters (Thryonomys swinderianus) in Ghana (2020)
  • Author(s): Adenyo C, Ohya K, Qiu Y, Takashima Y, Ogawa H, Matsumoto T, Thu MJ, Sato K, Kawabata H, Katayama Y, Omatsu T, Mizutani T, Fukushi H, Nonaka N, Katakura K, Inoue-Murayama M, Kayang B, Nakao R
  • Journal Title: Acta Trop Volume: 205 Pages: 105388-105388
  • DOI: 10.1016/j.actatropica.2020.105388
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First record and analysis of the COI gene of Cobboldia elephantis obtained from a captive Asian elephant from Myanmar. (2020)
  • Author(s): Chel HM, Nakao R, Ohsawa N, Oo ZM, Nonaka N, Katakura K
  • Journal Title: Parasitol Int Volume: 75 Pages: 102035-102035
  • DOI: 10.1016/j.parint.2019.102035
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphological and molecular identification of cyathostomine gastrointestinal nematodes of Murshidia and Quilonia species from Asian elephants in Myanmar. (2020)
  • Author(s): Chel HM, Iwaki T, Hmoon MM, Thaw YN, Soe NC, Win SY, Bawm S, Htun LL, Win MM, Oo ZM, Masum MA, Ichii O, Nakao R, Nonaka N, Katakura K
  • Journal Title: Int J Parasitol Parasites Wildl Volume: 13 Pages: 294-301
  • DOI: 10.1016/j.ijppaw.2020.03.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Complete Genome Sequence of Rickettsia asiatica Strain Maytaro1284, a Member of Spotted Fever Group Rickettsiae Isolated from an Ixodes ovatus Tick in Japan. (2019)
  • Author(s): Thu MJ, Qiu Y, Yamagishi J, Kusakisako K, Ogata S, Moustafa MAM, Isoda N, Sugimoto C, Katakura K, Nonaka N, Nakao R
  • Journal Title: Microbiol Resour Announc Volume: 8(37) Issue: 37 Pages: 37-37
  • DOI: 10.1128/mra.00886-19
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular characterization of species of the subgenus Mus from Myanmar. (2019)
  • Author(s): Zaw KMM, Thwe T, Shimada T, Theint SMM, Saing KM, Bawm S, Katakura K, Suzuki H
  • Journal Title: Zool Sci Volume: 36 Issue: 4 Pages: 299-305
  • DOI: 10.2108/zs180161
  • NAID: 40021964542
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar (2019)
  • Author(s): Fujisawa S, Murata S, Takehara M, Katakura K, Hmoon MM, Win SY, Ohashi K.
  • Journal Title: BMC Veterinary Research Volume: 15 Issue: 1 Pages: 261-261
  • DOI: 10.1186/s12917-019-2018-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Haematophagous mites on poultry farms in the Republic of the Union of Myanmar (2019)
  • Author(s): Takehara M, Murata S, Katakura K, Fujisawa S, Hmoon MM, Win SY, Bawm S, Htun LL, Aung YH, Win MM, Isezaki M, Maekawa N, Okagawa T, Konnai S, Ohashi K.
  • Journal Title: Heliyon Volume: 5 Issue: 4 Pages: e01544-e01544
  • DOI: 10.1016/j.heliyon.2019.e01544
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diversity of spotted fever group rickettsiae and their association with host ticks in Japan (2019)
  • Author(s): Thu MJ, Qiu Y, Matsuno K, Kajihara M, Mori-Kajihara A, Omori R, Monma N, Chiba K, Seto J, Gokuden M, Andoh M, Oosako H, Katakura K, Takada A, Sugimoto C, Isoda N, Nakao R
  • Journal Title: Scientific Reports Volume: 9(1) Issue: 1 Pages: 1500-1500
  • DOI: 10.1038/s41598-018-37836-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation of Rickettsia, Rickettsiella, and Spiroplasma from Questing Ticks in Japan Using Arthropod Cells (2019)
  • Author(s): Thu May June、Qiu Yongjin、Kataoka-Nakamura Chikako、Sugimoto Chihiro、Katakura Ken、Isoda Norikazu、Nakao Ryo
  • Journal Title: Vector-Borne and Zoonotic Diseases Volume: 印刷中 Issue: 7 Pages: 474-485
  • DOI: 10.1089/vbz.2018.2373
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recombination and purifying and balancing selection determine the evolution of major antigenic protein 1 (map 1) family genes in Ehrlichia ruminantium. (2019)
  • Author(s): Salim B, Amin M, Igarashi M, Ito K, Jongejan F, Katakura K, Sugimoto C, Nakao R.
  • Journal Title: Gene Volume: 683 Pages: 216-224
  • DOI: 10.1016/j.gene.2018.10.028
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Potential role of dogs as sentinels and reservoirs for piroplasms infecting equine and cattle in Riyadh City, Saudi Arabia (2019)
  • Author(s): Salim Bashir、Alanazi Abdullah D.、Omori Ryosuke、Alyousif Mohamed S.、Alanazi Ibrahim O.、Katakura Ken、Nakao Ryo
  • Journal Title: Acta Tropica Volume: 193 Pages: 78-83
  • DOI: 10.1016/j.actatropica.2019.02.029
  • NAID: 120006843068
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Haematophagous mites on poultry farms in the Republic of the Union of Myanmar (2019)
  • Author(s): Takehara Masaki, Murata Shiro, Katakura Ken, Fujisawa Sotaro, Hmoon Myint Myint, Win Shwe Yee, Bawm Saw, Htun Lat Lat, Aung Ye Htut, Win Mar Mar, Isezaki Masayoshi, Maekawa Naoya, Okagawa Tomohiro, Konnai Satoru, Ohashi Kazuhiko
  • Journal Title: Heliyon Volume: -
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation of Rickettsia, Rickettsiella, and Spiroplasma from questing ticks in Japan using arthropod cells. (2019)
  • Author(s): Thu May June、Qiu Yongjin, Kataoka-Nakamura Chikako, Sugimot Chihiro, Katakura Ken, Isoda Norikazu, Nakao Ryo
  • Journal Title: Ticks and Tick-borne Diseases Volume: -
  • NAID: 120006864496
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Potential of cell-free DNA as a screening marker for parasite infections in dog (2018)
  • Author(s): Akter Shirin、Nakao Ryo、Imasato Yuhei、Alam Mohammad Zahangir、Katakura Ken
  • Journal Title: Genomics Volume: - Issue: 4 Pages: 906-912
  • DOI: 10.1016/j.ygeno.2018.05.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia (2018)
  • Author(s): Qiu Yongjin、Kaneko Chiho、Kajihara Masahiro、Ngonda Saasa、Simulundu Edgar、Muleya Walter、Thu May J、Hang’ombe Bernard、Katakura Ken、Takada Ayato、Sawa Hirofumi、Simuunza Martin、Nakao Ryo
  • Journal Title: Ticks and Tick-borne Diseases Volume: 印刷中 Issue: 4 Pages: 988-995
  • DOI: 10.1016/j.ttbdis.2018.03.025
  • NAID: 120006626572
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First molecular detection of Theileria luwenshuni from goats in Myanmar (2018)
  • Author(s): Bawm Saw、Kakisaka Keita、Thu May June、Chel Hla Myet、Oo Yamon Min Nyunt、Soe Nyein Chan、Win Shwe Yee、Htun Lat Lat、Win Mar Mar、Suzuki Hitoshi、Nakao Ryo、Katakura Ken
  • Journal Title: Parasitology Research Volume: 117 Issue: 10 Pages: 3361-3364
  • DOI: 10.1007/s00436-018-6073-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic homogeneity of goat malaria parasites in Asia and Africa suggests their expansion with domestic goat host. (2018)
  • Author(s): Kaewthamasorn M, Takeda M, Saiwichai T, Gitaka JN, Tiawsirisup S, Imasato Y, Mossaad E, Sarani A, Kaewlamun W, Channumsin M, Chaiworakul S, Katepongpun W, Teeveerapunya S, Panthong J, Mureithi DK, Bawm S, Htun LL, Win MM, Ismail AA, Ibrahim AM, Suganuma K, Hakimi H, Nakao R, Katakura K, Asada M, Kaneko O
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 5827-5827
  • DOI: 10.1038/s41598-018-24048-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Occurrence of Coxiella burnetii, Ehrlichia canis, Rickettsia species and Anaplasma phagocytophilum-like bacterium in ticks collected from dogs and cats in South Africa (2017)
  • Author(s): Mtshali K, Nakao R, Sugimoto C, Thekisoe O
  • Journal Title: J S Afr Vet Assoc Volume: 88
  • DOI: 10.4102/jsava.v88i0.1390
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] マダニが保有するスピロプラズマ属細菌の多様性 (2019)
  • Author(s): 小方昌平、中尾亮、片倉賢、野中成晃
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] Sequences of two genes encoding Theileria parva CD8+ antigens reveal the presence of Muguga vaccine strain in non-vaccinated cattle in Malawi. (2019)
  • Author(s): Elisha Chatanga, Ryo Nakao, Kyoko Hayashida, Bashir Salim, Chihiro Sugimoto, Ken Katakura, Nariaki Nonaka
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] マダニとその共生微生物のハンティング (2018)
  • Author(s): 中尾 亮
  • Organizer: 第70回日本衛生動物学会大会
  • Invited
• [Presentation] マダニの共生微生物から探る病原体進化 (2018)
  • Author(s): 中尾 亮
  • Organizer: 第161回日本獣医学会学術集会
  • Invited
• [Presentation] Complete mitochondrial genome analysis of ticks (2018)
  • Author(s): Ryo Nakao, Gohta Kinoshita, Ayumi Saito, Yongjin Qiu, Keita Matsuno, Chihiro Sugimoto, Ken Katakura
  • Organizer: 14th International Congress of Parasitology (ICOPA 2018)
  • Int'l Joint Research
• [Presentation] Molecular detection of Marek’s disease virus in poultry farms in Myanmar (2018)
  • Author(s): Shiro Murata, Masaki Takehara, Ken Katakura, Myint Myint Hmoon, Shwe Yee Win, Masayoshi Isezaki, Satoru Konnai, Kazuhiko Ohashi
  • Organizer: The 12th International Symposium on Marek’s disease and Avian Herpesviruses
  • Int'l Joint Research
• [Presentation] Isolation of endosymbionts of ticks in Japan (2018)
  • Author(s): May June Thu, Yongjin Qiu, Chihiro Sugimoto, Ken Katakura, Norikazu Isoda, Ryo Nakao
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] ミャンマー連邦共和国における鶏の吸血性外部寄生虫の分布状況調査 (2018)
  • Author(s): 竹原昌生、村田史郎、片倉賢、Myint Myint Hmoon、Shwe Yee Win、Saw Bawm、Lat Lat Htun、Ye Htut Aung、Mar Mar Win、伊勢崎政美、今内覚、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] PCR detection and phylogenetic analysis of haemoprotozoan parasites in livestock in Myanmar by sequence analysis of selected target genes (2018)
  • Author(s): Ken Katakura, Saw Bawm, Hla Myet Chel, May June Thu, Lat Lat Htun, Yuhei Imasato, Ryo Nakao
  • Organizer: 14th International Conference on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (MEEGID XIV)
  • Int'l Joint Research
• [Presentation] Molecular detection and genetic diversity of canine Babesia parasites from Myanmar (2017)
  • Author(s): Bawm S, June TM, Htun LL, Win MM, Nakao R, Katakura K
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Detection of Cryptosporidium parasite in ruminants from highland area of Myanmar (2017)
  • Author(s): Bawm S, Moe KT, Chel HM, Htun LL, Nakao R, Katakura K
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Detection of Cryptosporidium parasite in ruminants from highland and lowland areas of Myanmar (2017)
  • Author(s): Bawm S, Moe K, Soe BK, Chel HM, Htun LL, Nakao R, Katakura K
  • Organizer: 2017 8th International Conference on Agriculture and Animal Science (ICAAS 2017)
  • Int'l Joint Research
• [Presentation] 寄生虫と人と動物と旅 (2017)
  • Author(s): 片倉 賢
  • Organizer: 第86回日本寄生虫学会大会
  • Invited
• [Presentation] マダニが持つ微生物の多様性：共生体から病原体まで (2017)
  • Author(s): 中尾 亮
  • Organizer: 第１７回北大若手研究者交流会
  • Invited
• [Presentation] Genetic diversity of Rickettsia spp. in ticks collected from Japan (2017)
  • Author(s): Thu MJ, Qiu Y, Sugimoto C, Isoda N, Nakao R
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] Coxiella-like endosymbiont as an evolutionary marker of ticks (2017)
  • Author(s): Nakao R, Saito A, Thu MJ, Qiu Y, Matsuno K, Sugimoto C, Katakura K
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] ミトゲノム解析によるマダニ系統分類法の開発 (2017)
  • Author(s): 中尾 亮、木下豪太、齋藤 歩、邱 永晋、松野啓太、杉本千尋、片倉 賢
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] マダニから分離されたスピロプラズマの全ゲノム解析 (2017)
  • Author(s): 柿阪圭太、中尾 亮、Thu MJ、邱 永晋、杉本千尋、片倉 賢
  • Organizer: 第160回日本獣医学会学術集会
• [Remarks] 北海道大学大学院獣医学研究院・獣医学部 獣医学大学院研究室一覧 寄生虫学教室
  • URL: https://www.vetmed.hokudai.ac.jp/research/detail/parasitology/
• [Remarks] 北海道大学大学院獣医学研究院・獣医学部 寄生虫学教室ホームページ
  • URL: https://www.vetmed.hokudai.ac.jp/organization/parasitol/
• [Remarks] 北海道大学大学院獣医学研究院・獣医学部 寄生虫学教室ホームページ
  • URL: https://www.vetmed.hokudai.ac.jp/organization/parasitol/index.html