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Realization of Micro Spectrophotometer and Measurement of Single Cell Spectra by Photothermal Optical Phase Shift Detection Method

Research Project

Project/Area Number 17H04883
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Shimizu Hisashi  東京大学, 大学院工学系研究科(工学部), 特任助教 (60631281)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsマイクロ流体デバイス / 光熱変換検出 / 光熱変換分光 / 非蛍光検出 / マイクロ流体工学 / 光ファイバー / 光熱変換分光法 / 吸光光度法 / マイクロ流体チップ / 光熱変換 / 吸光光度計 / 吸収スペクトル / 吸光度分析 / 単一細胞分析
Outline of Final Research Achievements

Microfluidic devices have become widespread as research tools in the fields of chemistry, biology, and medicine. However, it is challenging to directly measure light absorption using absorbance spectroscopy in the small spaces. Therefore, research has been conducted on photothermal spectroscopy, which detects the heat generation followed by light absorption, while measurement of absorption spectrum was difficult. Therefore, based on the photothermal optical phase shift (POPS) detection developed by our group, we constructed an interferometer by incorporating optical fibers into a microfluidic device. Guides for optical fibers with a measurement channel were fabricated on a glass substrate using hydrofluoric acid and plasma etching. The device was verified to work as an interferometer and a photothermal signal was detected using a laser light source as a proof of principle.

Academic Significance and Societal Importance of the Research Achievements

ガラス製マイクロ流体デバイスに光ファイバを用いた検出光学系を集積化することに初めて成功した。本研究で用いた干渉光学系はもちろん、より単純な熱レンズ検出光学系の集積化も期待できる。本研究実施中に並行して進めていたマイクロ流体化学プラントの研究が大きく進展したことから、プラントが正常に稼働していることの確認や、生産したポリマーや高機能化成品の品質評価などに応用できる可能性が生まれている。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (19 results)

All 2022 2021 2020 2018 2017

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 8 results)

  • [Journal Article] Photothermal spectroscopy and micro/nanofluidics2022

    • Author(s)
      Shimizu Hisashi、Chen Chihchen、Tsuyama Yoshiyuki、Tsukahara Takehiko、Kitamori Takehiko
    • Journal Title

      Journal of Applied Physics

      Volume: 132 Issue: 6 Pages: 060902-060902

    • DOI

      10.1063/5.0097665

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Numerical modeling of photothermal effect in nanofluidic channels2021

    • Author(s)
      Shimizu Hisashi、Kitamori Takehiko
    • Journal Title

      Microfluidics and Nanofluidics

      Volume: 25 Issue: 7 Pages: 1-8

    • DOI

      10.1007/s10404-021-02458-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Review of ultrasensitive readout for micro-/nanofluidic devices by thermal lens microscopy2021

    • Author(s)
      Chen Chihchen、Shimizu Hisashi、Kitamori Takehiko
    • Journal Title

      Journal of Optical Microsystems

      Volume: 1 Issue: 02 Pages: 020901-020901

    • DOI

      10.1117/1.jom.1.2.020901

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NUMERICAL AND EXPERIMENTAL INVESTIGATIONS OF PHOTOTHERMAL EFFECT IN NANOFLUIDIC CHANNELS2021

    • Author(s)
      Hisashi Shimizu and Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS 2021

      Volume: 1 Pages: 1557-1558

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Advances in Label-Free Detections for Nanofluidic Analytical Devices2020

    • Author(s)
      Thu Hac Huong Le, Hisashi Shimizu, Kyojiro Morikawa
    • Journal Title

      Micromachines

      Volume: 11 Issue: 10 Pages: 885-885

    • DOI

      10.3390/mi11100885

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrasensitive detection of nonlabelled bovine serum albumin using photothermal optical phase shift detection with UV excitation2020

    • Author(s)
      Shimizu Hisashi、Takeda Shigenori、Mawatari Kazuma、Kitamori Takehiko
    • Journal Title

      The Analyst

      Volume: 145 Issue: 7 Pages: 2580-2585

    • DOI

      10.1039/d0an00037j

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PHOTOTHERMAL OPTICAL PHASE SHIFT DETECTION USING OPTICAL FIBER2018

    • Author(s)
      Naoki Wada, Hisashi Shimizu, Kazuma Mawatari, Takehiko Kitamori
    • Journal Title

      Proceedings of MicroTAS 2018

      Volume: 1 Pages: 964-965

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Wavelength-Tunable Photothermal Optical Phase Shift Detection Device2017

    • Author(s)
      F. Sugino, H. Shimizu, M. Sakakura, K. Miura, K. Mawatari, T. Kitamori
    • Journal Title

      Proceedings of MicroTAS 2017

      Volume: 1 Pages: 569-570

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ON-GLASSCHIP INTEGRATION OF PHOTONICS FOR PHOTOTHERMAL OPTICAL PHASE SHIFT DETECTION2017

    • Author(s)
      K. Miyake, H. Shimizu, K. Mawatari, T. Kitamori
    • Journal Title

      Proceedings of MicroTAS 2017

      Volume: 1 Pages: 563-564

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] NUMERICAL AND EXPERIMENTAL INVESTIGATIONS OF PHOTOTHERMAL EFFECT IN NANOFLUIDIC CHANNELS2021

    • Author(s)
      Hisashi Shimizu and Takehiko Kitamori
    • Organizer
      MicroTAS 2021
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 有限要素法によるナノ流路内の光熱変換現象の数値解析2021

    • Author(s)
      清水久史、北森武彦
    • Organizer
      化学とマイクロ・ナノシステム学会第43回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] PHOTOTHERMAL OPTICAL PHASE SHIFT DETECTION USING OPTICAL FIBER2018

    • Author(s)
      Naoki Wada, Hisashi Shimizu, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ空間における吸光度分析に向けた波長可変光熱変換光位相差検出デバイスの開発2018

    • Author(s)
      杉野文俊、清水久史、坂倉政明、三浦清貴、馬渡和真、北森武彦
    • Organizer
      日本化学会 第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Planar integration of photothermal detection device using optical fiber2017

    • Author(s)
      H. Shimizu, K. Miyake, K. Mawatari, T. Kitamori
    • Organizer
      9th International Symposium on Microchemistry and Microsystems (ISMM 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ON-GLASSCHIP INTEGRATION OF PHOTONICS FOR PHOTOTHERMAL OPTICAL PHASE SHIFT DETECTION2017

    • Author(s)
      K. Miyake, H. Shimizu, K. Mawatari, T. Kitamori
    • Organizer
      The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PHOTOTHERMAL OPTICAL PHASE SHIFT DETECTION DEVICE FOR MEASUREMENT OF ABSORPTION SPECTRUM IN MICROSPACE2017

    • Author(s)
      F. Sugino, H. Shimizu, M. Sakakura, K. Miura, K. Mawatari, T. Kitamori
    • Organizer
      The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Wavelength-Tunable Photothermal Optical Phase Shift Detection Device2017

    • Author(s)
      F. Sugino, H. Shimizu, M. Sakakura, K. Miura, K. Mawatari, T. Kitamori
    • Organizer
      46th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Integration of optical fiber for photothermal detection devices2017

    • Author(s)
      H. Shimizu, K. Miyake, K. Mawatari, T. Kitamori
    • Organizer
      46th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 吸収スペクトルを取得可能な光熱変換光位相差検出デバイスの開発2017

    • Author(s)
      杉野文俊、清水久史、坂倉政明、三浦清貴、馬渡和真、北森武彦
    • Organizer
      化学とマイクロ・ナノシステム学会 第36回研究会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2024-01-30  

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