| Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we induced mesoderm consisting of heterogeneous cell populations from human iPS cells. To elucidate cell fate determination in mesoderm differentiation, we performed single-cell RNA-seq analysis upon above differentiation system. The results showed that human iPS cells differentiate into paraxial mesoderm, intermediate mesoderm, lateral plate mesoderm, and neural crest cells. We have succeeded in visualizing the process of differentiation and precise timings of fate determination in each mesoderm. Furthermore, the signaling pathways that may act in fate determination in each mesodermal cell were identified by ligand-receptor analysis.
|
