Identification of host factors involved in infection of phytoplasma.

• Project/Area Number: 17H06618
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant protection science
• Research Institution: The University of Tokyo
• Principal Investigator: Kitazawa Yugo 東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (50803160)
• Project Period (FY): 2017-08-25 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ファイトプラズマ / 感染機構 / 宿主因子

Outline of Final Research Achievements

In this project, we constructed a highly efficient inoculum system of phytoplasma, a non-cultivating phytopathogenic bacteria. Using this inoculation system, phytoplasma was inoculated into Arabidopsis plants with various genetic background. As a result, it was suggested that plant hormone contributes to the symptom development by phytoplasma. In addition, we constructed a drug screening system for phytoplasma, and identified several novel antimicrobials effective for phytoplasma.

Academic Significance and Societal Importance of the Research Achievements

本研究では、ファイトプラズマの病徴の進展と植物ホルモンとの間の関連性が示唆されたが、これはファイトプラズマという特異な細菌がどのように宿主と関わり感染を成立させるかを明らかにする上で重要な知見である。加えて、本研究で確立した接種系により、これまで研究手法が限られていたファイトプラズマの研究が容易になることが見込まれる。したがって、本研究を基盤として、ファイトプラズマの感染機構や防除法に関する研究が一層進展することが期待される。

Research Products

• [Journal Article] Crystal structure of phyllogen, a phyllody-inducing effector protein of phytoplasma (2019)
  • Author(s): Iwabuchi Nozomu、Maejima Kensaku、Kitazawa Yugo、Miyatake Hideyuki、Nishikawa Masanobu、Tokuda Ryosuke、Koinuma Hiroaki、Miyazaki Akio、Nijo Takamichi、Oshima Kenro、Yamaji Yasuyuki、Namba Shigetou
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 印刷中 Issue: 4 Pages: 952-957
  • DOI: 10.1016/j.bbrc.2019.04.060
  • Peer Reviewed
• [Journal Article] Comprehensive screening of antimicrobials to control phytoplasma diseases using an in vitro plant?phytoplasma co-culture system (2018)
  • Author(s): Tanno Kazuyuki、Maejima Kensaku、Miyazaki Akio、Koinuma Hiroaki、Iwabuchi Nozomu、Kitazawa Yugo、Nijo Takamichi、Hashimoto Masayoshi、Yamaji Yasuyuki、Namba Shigetou
  • Journal Title: Microbiology Volume: 164 Issue: 8 Pages: 1048-1058
  • DOI: 10.1099/mic.0.000681
  • Peer Reviewed / Open Access
• [Journal Article] First report of ‘Candidatus Phytoplasma pruni’ infecting cassava in Japan (2018)
  • Author(s): Koinuma Hiroaki、Miyazaki Akio、Wakaki Renya、Fujimoto Yuji、Iwabuchi Nozomu、Nijo Takamichi、Kitazawa Yugo、Shigaki Toshiro、Maejima Kensaku、Yamaji Yasuyuki、Namba Shigetou
  • Journal Title: Journal of General Plant Pathology Volume: 84 Issue: 4 Pages: 300-304
  • DOI: 10.1007/s10327-018-0787-2
  • Peer Reviewed
• [Journal Article] Complete genome sequence of the first isolate of hibiscus latent Singapore virus detected in Japan (2018)
  • Author(s): Yoshida T., Kitazawa Y., Neriya Y., Hosoe N., Fujimoto Y., Hagiwara-Komoda Y., Maejima K., Yamaji Y., Namba S.
  • Journal Title: Genome Announcements Volume: 6 Issue: 7
  • DOI: 10.1128/genomea.00054-18
  • Peer Reviewed / Open Access
• [Journal Article] Genome-Wide Analysis of the Transcription Start Sites and Promoter Motifs of Phytoplasmas (2017)
  • Author(s): Nijo Takamichi、Neriya Yutaro、Koinuma Hiroaki、Iwabuchi Nozomu、Kitazawa Yugo、Tanno Kazuyuki、Okano Yukari、Maejima Kensaku、Yamaji Yasuyuki、Oshima Kenro、Namba Shigetou
  • Journal Title: DNA and Cell Biology Volume: 36 Issue: 12 Pages: 1081-1092
  • DOI: 10.1089/dna.2016.3616
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First report of ‘Candidatus Phytoplasma malaysianum’ associated with Elaeocarpus yellows of Elaeocarpus zollingeri (2017)
  • Author(s): Iwabuchi Nozomu、Endo Ai、Kameyama Norikazu、Satoh Masaya、Miyazaki Akio、Koinuma Hiroaki、Kitazawa Yugo、Maejima Kensaku、Yamaji Yasuyuki、Oshima Kenro、Namba Shigetou
  • Journal Title: Journal of General Plant Pathology Volume: 84 Issue: 2 Pages: 160-164
  • DOI: 10.1007/s10327-017-0761-4
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 感染植物由来DNAからのファイトプラズマDNA濃縮系の検討 (2019)
  • Author(s): 二條貴通・前島健作・鯉沼宏章・岩渕望・徳田遼佑・西川雅展・北沢優悟・山次康幸・難波成任
  • Organizer: 平成31年度日本植物病理学会本大会
• [Presentation] Phyllody induction in diverse plant species by the phytoplasma effector phyllogen through degradation of host floral MADS-domain transcription factors (2018)
  • Author(s): Nozomu Iwabuchi, Yugo Kitazawa, Yuji Fujimoto, Hiroaki Koinuma, Takamichi Nijo, Tetsuya Yoshida, Yukari Okano, Kensaku Maejima, Yasuyuki Yamaji, Kenro Oshima, Shigetou Namba
  • Organizer: 22nd Congress of the International Organization for Mycoplasmology
  • Int'l Joint Research
• [Presentation] First characterization of transcription start sites of phytoplasma (2018)
  • Author(s): Kensaku Maejima, Takamichi Nijo, Yuji Fujimoto, Naoi Hosoe, Yutaro Neriya, Hiroaki Koinuma, Nozomu Iwabuchi, Yugo Kitazawa, Yukari Okano, Yasuyuki Yamaji, Kenro Oshima, Shigetou Namba
  • Organizer: Joint Congress of The 7th Meeting of the Asian Organization for Mycoplasmology, The 45th Meeting of the Japanese Society of Mycoplasmology
  • Int'l Joint Research
• [Presentation] Phyllogen induces phyllody symptoms in diverse plant species through degradation of their floral MADS domain transcription factors (2018)
  • Author(s): Nozomu Iwabuchi, Yugo Kitazawa, Yuji Fujimoto, Naoi Hosoe, Hiroaki Koinuma, Takamichi Nijo, Tetsuya Yoshida, Yukari Okano, Kensaku Maejima, Yasuyuki Yamaji, Kenro Oshima, Shigetou Namba
  • Organizer: Joint Congress of The 7th Meeting of the Asian Organization for Mycoplasmology, The 45th Meeting of the Japanese Society of Mycoplasmology
  • Int'l Joint Research
• [Presentation] ファイトプラズマのRNA転写開始点の網羅的解析による非コードRNAの検出 (2018)
  • Author(s): 鯉沼宏章・二條貴通・煉谷裕太朗・岩渕望・北沢優悟・岡野夕香里・前島健作・山次康幸・大島研郎・難波成任
  • Organizer: 平成30年度日本植物病理学会本大会
• [Presentation] ファイトプラズマの転写開始点上流に見いだされるプロモーター配列の解析 (2018)
  • Author(s): 二條貴通・鯉沼宏章・煉谷裕太朗・岩渕望・北沢優悟・岡野夕香里・前島健作・山次康幸・大島研郎・難波成任
  • Organizer: 平成30年度日本植物病理学会本大会