Effects of maternal separation on behaviors under group-housed conditions by a novel behavior analysis system and investigation of related brain regions

• Project/Area Number: 17H07032
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Hygiene and public health
• Research Institution: Nara Medical University
• Principal Investigator: ENDO Nozomi 奈良県立医科大学, 医学部, 研究助教 (90802819)
• Project Period (FY): 2017-08-25 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マウスモデル / 集団飼育 / 社会性 / 社会行動 / 母子分離 / 環境要因 / 自閉症 / 行動神経科学 / マウス / 集団飼育環境 / モデル動物 / 神経科学 / 行動学

Outline of Final Research Achievements

In this study, we investigated what influence the early life growth environment had on the behavior in the social group after growth, and aimed to elucidate its neural basis. Using a unique test method that enables long-term behavioral testing in group housing condition, we clarified that maternal separation during lactation and isolation rearing after weaning affect the activity and social behavior in the group housing. In addition, we identified behavioral phenotypes in model mice for autism.

Academic Significance and Societal Importance of the Research Achievements

本研究では独自の試験法により、幼少期の成育環境が成長後の集団内における行動様式に影響を与えることを明らかにした。また、自閉症モデルマウスの集団内における行動表現型を明らかにした。今後、これら行動学的異常の関係およびその神経基盤を解明することで、予防・治療法の開発など臨床応用につながる基礎的知見となる。

Research Products

• [Journal Article] The role of prostaglandin E2 receptor EP1 in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced neonatal hydronephrosis in mice (2019)
  • Author(s): Aida-Yasuoka Keiko、Nishimura Noriko、Fujisawa Nozomi、Endo Nozomi、Narumiya Shuh、Tohyama Chiharu
  • Journal Title: Toxicology Volume: 415 Pages: 10-17
  • DOI: 10.1016/j.tox.2019.01.006
  • Peer Reviewed
• [Journal Article] Characterization of behavioral phenotypes in the BTBR <i>T⁺ Itpr3^tf</i>/J mouse model of autism spectrum disorder under social housing conditions using the multiple animal positioning system (2019)
  • Author(s): Endo N, Makinodan M*, Somayama N, Komori T, Kishimoto T, Nishi M*
  • Journal Title: Experimental Animals Volume: 68 Issue: 3 Pages: 319-330
  • DOI: 10.1538/expanim.18-0177
  • NAID: 130007689880
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Neurochemical evidence for differential effects of acute and repeated oxytocin administration (2018)
  • Author(s): Benner Seico、Aoki Yuta、Watanabe Takamitsu、Endo Nozomi、Abe Osamu、Kuroda Miho、Kuwabara Hitoshi、Kawakubo Yuki、Takao Hidemasa、Kunimatsu Akira、Kasai Kiyoto、Bito Haruhiko、Kakeyama Masaki、Yamasue Hidenori
  • Journal Title: Molecular Psychiatry Volume: － Issue: 2 Pages: 249-249
  • DOI: 10.1038/s41380-018-0249-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Multiple animal positioning system shows that socially-reared mice influence the social proximity of isolation-reared cagemates. (2018)
  • Author(s): Endo N, Ujita W, Fujiwara T, Miyauchi H, Mishima H, Makino Y, Hashimoto L, Oyama, H, Makinodan M, Nishi M, Tohyama C, Kakeyama M*
  • Journal Title: Communications Biology Volume: 1 Issue: 1 Pages: 225-225
  • DOI: 10.1038/s42003-018-0213-5
  • Peer Reviewed / Open Access
• [Journal Article] Mice lacking a functional NMDA receptor exhibit social subordination in a group-housed environment (2017)
  • Author(s): Ujita Waka、Kohyama-Koganeya Ayako、Endo Nozomi、Saito Toki、Oyama Hiroshi
  • Journal Title: The FEBS Journal Volume: 285 Issue: 1 Pages: 188-196
  • DOI: 10.1111/febs.14334
  • Peer Reviewed / Open Access
• [Presentation] ハイコストハイリターン/ローコストローリターンの選択. (2019)
  • Author(s): 杣山奈実、遠藤のぞみ、小森崇史、西真弓
  • Organizer: 第124回日本解剖学会総会・全国学術集会
• [Presentation] The effects of repeated-social defeat stress on social behavior and decision-making related to reward acquisition in mice. (2019)
  • Author(s): Nami Somayama, Nozomi Endo, Takashi Komori, Mamiko Okuda, Hitoshi Nozu, Mayumi Nishi
  • Organizer: The 3rd Sino-Japan Symposium on the frontier of Behavioral Neuroendocrinology.
  • Int'l Joint Research
• [Presentation] Effects of early life adverse experiences on the brain: implications from maternal separation in mice. (2019)
  • Author(s): T. Sasagawa, N. Horii-Hayashi, N. Endo, M. Nishi.
  • Organizer: The 3rd Sino-Japan Symposium on the frontier of Behavioral Neuroendocrinology.
  • Int'l Joint Research
• [Presentation] マウス脳におけるペリニューロナルネットの時空間特異的形成：WFA染色による解析. (2018)
  • Author(s): 堀井謹子、遠藤のぞみ、笹川誉世、西真弓
  • Organizer: 第59回日本組織細胞化学会総会・学術集会
• [Presentation] うつ病モデルマウスにおける“意欲”の多角的評価. (2018)
  • Author(s): 杣山奈実、遠藤のぞみ、小森崇史、西真弓
  • Organizer: 第29回日本行動神経内分泌研究会
• [Presentation] Characterization on of usual behaviors under group-housing conditions by the Multiple Animals Positioning System in the BTBR T+ /J mouse model of autism. (2018)
  • Author(s): Nozomi Endo, Manabu Makinodan, Noriko Horii, Nami Somayama, Takashi Komori, Toshifumi Kishimoto, Mayumi Nishi
  • Organizer: 第41回日本神経科学大会
• [Presentation] 妊娠中の受動喫煙が子供の摂食行動・嗜好性行動に及ぼす影響の分子基盤の解明 (2018)
  • Author(s): 西真弓、遠藤のぞみ、堀井謹子
  • Organizer: 公益財団法人 喫煙科学研究財団 第33回 平成29年度助成研究発表会
• [Presentation] Effects of early life stress on reward seeking behaviors. (2018)
  • Author(s): Mayumi Nishi, Nozomi Endo, Takayo Mannari
  • Organizer: 8th Asia Pacific International Congress of Anatomists and 68th Korean Associating of Anatomists.
  • Int'l Joint Research
• [Presentation] 集団飼育環境における自閉症モデルマウスの行動解析 (2018)
  • Author(s): 遠藤のぞみ、牧之段学、堀井謹子、杣山奈実、小森崇史、岸本年史、西真弓
  • Organizer: 第123回 日本解剖学会総会・全国学術集会
• [Presentation] マウスにおける胎児期受動喫煙が摂食、情動行動に及ぼす影響 (2018)
  • Author(s): 蜂谷奈都子、笹川誉世、堀井謹子、遠藤のぞみ、西真弓
  • Organizer: 第123回 日本解剖学会総会・全国学術集会
• [Presentation] 新規行動解析システムによる社会的集団における自閉症モデルマウスの行動表現型解析 (2017)
  • Author(s): 遠藤のぞみ、牧之段学、堀井謹子、杣山奈実、小森崇史、岸本年史、西真弓
  • Organizer: 第93回 日本解剖学会学術集会・近畿支部