Functional analysis of genes responsible for neurological diseases by conditional mutagenesis in a single generation

• Project/Area Number: 17K01972
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic / Social brain science
• Research Institution: Niigata University
• Principal Investigator: Abe Manabu 新潟大学, 脳研究所, 准教授 (10334674)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 神経疾患原因遺伝子 / ゲノム編集 / コンディショナル遺伝子発現制御

Outline of Final Research Achievements

In this project, I attempted to develop a novel method for the regulation of conditional gene expression using the FLEx system and genomes derived from different species. A total of six knock-in mouse strains were established using the novel method. We also developed a method of producing genetically engineered mice. In the genome editing of early mouse embryos using the electroporation method, we were able to establish a technology that enables the knock-in of longer sequences more easily. One of the objectives of this study is to elucidate some aspects of the molecular mechanisms of cerebellar development, focusing on two genes that cause neurological diseases, Car8, Eef1a2, and their related molecules. Although we did not obtain much information on the molecular mechanism of cerebellar development, we believe that we were able to achieve remarkable results in terms of the regulation of gene expression and the development of technology for producing genetically modified mice.

Academic Significance and Societal Importance of the Research Achievements

本研究においてゲノム編集による遺伝子改変動物作製について技術的にさらに向上させたことで多くの生命科学研究の推進に寄与することができると期待され、また、遺伝子発現制御法の改良は遺伝子機能の評価をより正確にするという点で学術的意義は高い。さらに、一系統のマウスを希少神経疾患であるTimothy症候群の病態モデルとして研究代表者が発明者の一人となり特許出願したことから、本研究成果を社会へ還元できる可能性も高い。

Research Products

• [Int'l Joint Research] Aarhus大学/DANDRITE研究所(デンマーク)
• [Journal Article] Loss of <i>Atg2b</i> and <i>Gskip</i> Impairs the Maintenance of the Hematopoietic Stem Cell Pool Size (2022)
  • Author(s): Sakai Shun-suke、Hasegawa Atsushi、Ishimura Ryosuke、Tamura Naoki、Kageyama Shun、Komatsu-Hirota Satoko、Abe Manabu、Ling Yiwei、Okuda Shujiro、Funayama Manabu、Kikkawa Mika、Miura Yoshiki、Sakimura Kenji、Narita Ichiei、Waguri Satoshi、Shimizu Ritsuko、Komatsu Masaaki
  • Journal Title: Molecular and Cellular Biology Volume: 42 Issue: 1 Pages: 1-17
  • DOI: 10.1128/mcb.00024-21
  • Peer Reviewed / Open Access
• [Journal Article] A comparative analysis of kainate receptor GluK2 and GluK5 knockout mice in a pure genetic background (2021)
  • Author(s): Iida Izumi、Konno Kohtarou、Natsume Rie、Abe Manabu、Watanabe Masahiko、Sakimura Kenji、Terunuma Miho
  • Journal Title: Behavioural Brain Research Volume: 405 Pages: 113194-113194
  • DOI: 10.1016/j.bbr.2021.113194
  • Peer Reviewed / Open Access
• [Journal Article] Kv11 (ether-a-go-go-related gene) voltage-dependent K + channels promote resonance and oscillation of subthreshold membrane potentials (2021)
  • Author(s): Matsuoka Toshinori, Yamasaki Miwako, Abe Manabu, Matsuda Yukiko, Morino Hiroyuki, Kawakami Hideshi, Sakimura Kenji, Watanabe Masahiko, Hashimoto Kouichi
  • Journal Title: The Journal of Physiology Volume: 599(2) Issue: 2 Pages: 547-569
  • DOI: 10.1113/jp280342
  • Peer Reviewed / Open Access
• [Journal Article] Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice. (2021)
  • Author(s): Miyata S, Kakizaki T, Fujihara K, Obinata H, Hirano T, Nakai J, Tanaka M, Itohara S, Watanabe M, Tanaka KF, Abe M, Sakimura K, Yanagawa Y.
  • Journal Title: Mol Brain Volume: 14 Issue: 1 Pages: 5-5
  • DOI: 10.1186/s13041-020-00713-2
  • Peer Reviewed / Open Access
• [Journal Article] Central dopamine D2 receptors regulate plasma glucose levels in mice through autonomic nerves (2020)
  • Author(s): Ikeda Hiroko、Yonemochi Naomi、Mikami Risa、Abe Manabu、Kawamura Meiko、Natsume Rie、Sakimura Kenji、Waddington John L.、Kamei Junzo
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 22347-22347
  • DOI: 10.1038/s41598-020-79292-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of hepatic oxidative stress by voltage‐gated proton channels (Hv1/VSOP) in Kupffer cells and its potential relationship with glucose metabolism (2020)
  • Author(s): Kawai Takafumi、Kayama Kento、Tatsumi Shoki、Akter Sharmin、Miyawaki Nana、Okochi Yoshifumi、Abe Manabu、Sakimura Kenji、Yamamoto Hiroyasu、Kihara Shinji、Okamura Yasushi
  • Journal Title: The FASEB Journal Volume: 34 Issue: 12 Pages: 15805-15821
  • DOI: 10.1096/fj.202001056rrr
  • Peer Reviewed / Open Access
• [Journal Article] Generation of Lungs by Blastocyst Complementation in Apneumic Fgf10-Deficient Mice (2020)
  • Author(s): Kitahara Akihiko、Ran Qingsong、Oda Kanako、Yasue Akihiro、Abe Manabu、Ye Xulu、Sasaoka Toshikuni、Tsuchida Masanori、Sakimura Kenji、Ajioka Yoichi、Saijo Yasuo、Zhou Qiliang
  • Journal Title: Cell Reports Volume: 31 Issue: 6 Pages: 107626-107626
  • DOI: 10.1016/j.celrep.2020.107626
  • Peer Reviewed / Open Access
• [Journal Article] A discrete neuronal circuit induces a hibernation-like state in rodents (2020)
  • Author(s): Takahashi TM, Sunagawa GA, Soya S, Abe M, Sakurai K, Ishikawa K, Yanagisawa M, Hama H, Hasegawa E, Miyawaki A, Sakimura K, Takahashi M, Sakurai T.
  • Journal Title: Nature Volume: - Issue: 7814 Pages: 109-114
  • DOI: 10.1038/s41586-020-2163-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Decreased Proliferation in the Neurogenic Niche, Disorganized Neuroblast Migration, and Increased Oligodendrogenesis in Adult Netrin-5-Deficient Mice (2020)
  • Author(s): Ikegaya Shunsuke、Iga Yurika、Mikawa Sumiko、Zhou Li、Abe Manabu、Sakimura Kenji、Sato Kohji、Yamagishi Satoru
  • Journal Title: Frontiers in Neuroscience Volume: 14 Pages: 570974-570974
  • DOI: 10.3389/fnins.2020.570974
  • Peer Reviewed / Open Access
• [Journal Article] GluD1 knockout mice with a pure C57BL/6N background show impaired fear memory, social interaction, and enhanced depressive-like behavior (2020)
  • Author(s): Nakamoto Chihiro、Kawamura Meiko、Nakatsukasa Ena、Natsume Rie、Takao Keizo、Watanabe Masahiko、Abe Manabu、Takeuchi Tomonori、Sakimura Kenji
  • Journal Title: PLOS ONE Volume: 15 Issue: 2 Pages: 0229288-0229288
  • DOI: 10.1371/journal.pone.0229288
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain (2019)
  • Author(s): Nakamoto Chihiro、Konno Kohtarou、Miyazaki Taisuke、Nakatsukasa Ena、Natsume Rie、Abe Manabu、Kawamura Meiko、Fukazawa Yugo、Shigemoto Ryuichi、Yamasaki Miwako、Sakimura Kenji、Watanabe Masahiko
  • Journal Title: Journal of Comparative Neurology Volume: 528 Issue: 6 Pages: 1003-1027
  • DOI: 10.1002/cne.24792
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging (2019)
  • Author(s): Sugiyama Eiji、Guerrini Matteo M.、Honda Kurara、Hattori Yuko、Abe Manabu、K?llback Patrik、Andr?n Per E.、Tanaka Kenji F.、Setou Mitsutoshi、Fagarasan Sidonia、Suematsu Makoto、Sugiura Yuki
  • Journal Title: iScience Volume: 20 Pages: 359-372
  • DOI: 10.1016/j.isci.2019.09.036
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas (2019)
  • Author(s): Shimizu Takeshi、Osanai Yasuyuki、Tanaka Kenji F.、Thai Truc Quynh、Abe Manabu、Natsume Rie、Sakimura Kenji、Ikenaka Kazuhiro
  • Journal Title: Journal of Neurochemistry Volume: 2019 Issue: 2 Pages: 158-172
  • DOI: 10.1111/jnc.14657
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ELKS/Voltage-dependent Ca2+ channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β-Cells. (2019)
  • Author(s): Ohara-Imaizumi, M., Aoyagi, K., Yamauchi, H., Yoshida, M., Mori, M.X., Hida, Y., Tran, H.N., Ohkura, M., Abe, M., Akimoto, Y., Nakamichi, Y., Nishiwaki, C., Kawakami, H., Hara, K., Sakimura, K., Nagamatsu, S., Mori, Y., Nakai, J., Kakei, M., and Ohtsuka, T.
  • Journal Title: Cell Reports Volume: 26 Issue: 5 Pages: 1213-1226
  • DOI: 10.1016/j.celrep.2018.12.106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mice deficient in the C-terminal domain of TAR DNA-binding protein 43 develop age-dependent motor dysfunction associated with impaired Notch1?Akt signaling pathway (2019)
  • Author(s): Nishino Kohei、Watanabe Seiji、Shijie Jin、Murata Yuri、Oiwa Kotaro、Komine Okiru、Endo Fumito、Tsuiji Hitomi、Abe Manabu、Sakimura Kenji、Mishra Amit、Yamanaka Koji
  • Journal Title: Acta Neuropathologica Communications Volume: 7 Issue: 1 Pages: 118-118
  • DOI: 10.1186/s40478-019-0776-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Autophosphorylation of F-actin binding domain of CaMKIIβ is required for fear learning (2019)
  • Author(s): Kim Karam、Suzuki Akio、Kojima Hiroto、Kawamura Meiko、Miya Ken、Abe Manabu、Yamada Ikuko、Furuse Tamio、Wakana Shigenaru、Sakimura Kenji、Hayashi Yasunori
  • Journal Title: Neurobiology of Learning and Memory Volume: 157 Pages: 86-95
  • DOI: 10.1016/j.nlm.2018.12.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Differential Roles of Each Orexin Receptor Signaling in Obesity (2019)
  • Author(s): Kakizaki Miyo、Tsuneoka Yousuke、Takase Kenkichi、Kim Staci J.、Choi Jinhwan、Ikkyu Aya、Abe Manabu、Sakimura Kenji、Yanagisawa Masashi、Funato Hiromasa
  • Journal Title: iScience Volume: 20 Pages: 1-13
  • DOI: 10.1016/j.isci.2019.09.003
  • NAID: 120007132695
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics (2019)
  • Author(s): Inoue M, Takeuchi A, Manita S, Horigane S-i, Sakamoto M, Kawakami R, Yamaguchi K, Otomo K, Yokoyama H, Kim R, Yokoyama T, Takemoto-Kimura S, Abe M, Okamura M, Kondo Y, Quirin S, Ramakrishnan C, Imamura T, Sakimura K, Nemoto T, Kano M, Fujii H, Deisseroth K, Kitamura K, Bito H.
  • Journal Title: Cell Volume: 177 Issue: 5 Pages: 1346
  • DOI: 10.1016/j.cell.2019.04.007
  • URL: https://localhost/en/publications/a48843c1-543d-4414-a93c-3cab0e1df627
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity (2019)
  • Author(s): Doi Masao、Shimatani Hiroyuki、Atobe Yuta、Murai Iori、Hayashi Hida、Takahashi Yukari、Fustin Jean-Michel、Yamaguchi Yoshiaki、Kiyonari Hiroshi、Koike Nobuya、Yagita Kazuhiro、Lee Choogon、Abe Manabu、Sakimura Kenji、Okamura Hitoshi
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 2563-2563
  • DOI: 10.1038/s41467-019-10532-2
  • NAID: 120006644061
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice (2019)
  • Author(s): Chowdhury Srikanta、Hung Chi Jung、Izawa Shuntaro、Inutsuka Ayumu、Kawamura Meiko、Kawashima Takashi、Bito Haruhiko、Imayoshi Itaru、Abe Manabu、Sakimura Kenji、Yamanaka Akihiro
  • Journal Title: eLife Volume: 8 Pages: 1-25
  • DOI: 10.7554/elife.44927
  • NAID: 120006888021
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice (2019)
  • Author(s): Chowdhury Srikanta、Matsubara Takanori、Miyazaki Toh、Ono Daisuke、Fukatsu Noriaki、Abe Manabu、Sakimura Kenji、Sudo Yuki、Yamanaka Akihiro
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.44928
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Serotonergic Input to Orexin Neurons Plays a Role in Maintaining Wakefulness and REM Sleep Architecture (2018)
  • Author(s): Saito Yuki C.、Tsujino Natsuko、Abe Manabu、Yamazaki Maya、Sakimura Kenji、Sakurai Takeshi
  • Journal Title: Frontiers in Neuroscience Volume: 12 Pages: 892-892
  • DOI: 10.3389/fnins.2018.00892
  • NAID: 120007133662
  • Peer Reviewed / Open Access
• [Journal Article] Perturbed expression pattern of the immediate early gene Arc in the dentate gyrus of GluA1 C‐terminal palmitoylation‐deficient mice (2018)
  • Author(s): Itoh Masayuki、Okuno Hiroyuki、Yamada Daisuke、Yamashita Mariko、Abe Manabu、Natsume Rie、Kaizuka Toshie、Sakimura Kenji、Hoshino Mikio、Mishina Masayoshi、Wada Keiji、Sekiguchi Masayuki、Hayashi Takashi
  • Journal Title: Neuropsychopharmacology Reports Volume: 39 Issue: 1 Pages: 61-66
  • DOI: 10.1002/npr2.12044
  • Peer Reviewed / Open Access
• [Journal Article] Different Roles of Sphingosine Kinase 1 and 2 in Pancreatic Cancer Progression (2018)
  • Author(s): Yuza Kizuki、Nakajima Masato、Nagahashi Masayuki、Tsuchida Junko、Hirose Yuki、Miura Kohei、Tajima Yosuke、Abe Manabu、Sakimura Kenji、Takabe Kazuaki、Wakai Toshifumi
  • Journal Title: Journal of Surgical Research Volume: 232 Pages: 186-194
  • DOI: 10.1016/j.jss.2018.06.019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distribution of Caskin1 protein and phenotypic characterization of its knockout mice using a comprehensive behavioral test battery (2018)
  • Author(s): Katano Tayo、Takao Keizo、Abe Manabu、Yamazaki Maya、Watanabe Masahiko、Miyakawa Tsuyoshi、Sakimura Kenji、Ito Seiji
  • Journal Title: Molecular Brain Volume: 11 Issue: 1 Pages: 63-63
  • DOI: 10.1186/s13041-018-0407-2
  • Peer Reviewed / Open Access
• [Journal Article] Deficiency of AMPAR?Palmitoylation Aggravates Seizure Susceptibility (2018)
  • Author(s): Itoh Masayuki、Yamashita Mariko、Kaneko Masaki、Okuno Hiroyuki、Abe Manabu、Yamazaki Maya、Natsume Rie、Yamada Daisuke、Kaizuka Toshie、Suwa Reiko、Sakimura Kenji、Sekiguchi Masayuki、Wada Keiji、Hoshino Mikio、Mishina Masayoshi、Hayashi Takashi
  • Journal Title: The Journal of Neuroscience Volume: 38 Issue: 47 Pages: 10220-10235
  • DOI: 10.1523/jneurosci.1590-18.2018
  • Peer Reviewed / Open Access
• [Journal Article] Deletion of exons encoding carboxypeptidase domain of Nna1 results in Purkinje cell degeneration (pcd) phenotype (2018)
  • Author(s): Zhou Li、Hossain M. Ibrahim、Yamazaki Maya、Abe Manabu、Natsume Rie、Konno Kohtaro、Kageyama Shun、Komatsu Masaaki、Watanabe Masahiko、Sakimura Kenji、Takebayashi Hirohide
  • Journal Title: Journal of Neurochemistry Volume: 147 Issue: 4 Pages: 557-572
  • DOI: 10.1111/jnc.14591
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance (2018)
  • Author(s): Hagiwara Akari、Kitahara Yosuke、Grabner Chad Paul、Vogl Christian、Abe Manabu、Kitta Ryo、Ohta Keisuke、Nakamura Keiichiro、Sakimura Kenji、Moser Tobias、Nishi Akinori、Ohtsuka Toshihisa
  • Journal Title: The Journal of Cell Biology Volume: 217 Issue: 11 Pages: 3993-4006
  • DOI: 10.1083/jcb.201704076
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cathepsin C modulates myelin oligodendrocyte glycoprotein‐induced experimental autoimmune encephalomyelitis (2018)
  • Author(s): Durose Wilaiwan Wisessmith、Shimizu Takahiro、Li JiaYi、Abe Manabu、Sakimura Kenji、Chetsawang Banthit、Tanaka Kenji F.、Suzumura Akio、Tohyama Koujiro、Ikenaka Kazuhiro
  • Journal Title: Journal of Neurochemistry Volume: 148 Issue: 3 Pages: 413-425
  • DOI: 10.1111/jnc.14581
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ablation of Central Serotonergic Neurons Decreased REM Sleep and Attenuated Arousal Response (2018)
  • Author(s): Iwasaki Kanako、Komiya Haruna、Kakizaki Miyo、Miyoshi Chika、Abe Manabu、Sakimura Kenji、Funato Hiromasa、Yanagisawa Masashi
  • Journal Title: Frontiers in Neuroscience Volume: 12 Pages: 535-535
  • DOI: 10.3389/fnins.2018.00535
  • NAID: 120007133025
  • Peer Reviewed / Open Access
• [Journal Article] Higher visual responses in the temporal cortex of mice (2018)
  • Author(s): Nishio Nana、Tsukano Hiroaki、Hishida Ryuichi、Abe Manabu、Nakai Junichi、Kawamura Meiko、Aiba Atsushi、Sakimura Kenji、Shibuki Katsuei
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 11136-11136
  • DOI: 10.1038/s41598-018-29530-3
  • Peer Reviewed / Open Access
• [Journal Article] Microglia permit climbing fiber elimination by promoting GABAergic inhibition in the developing cerebellum (2018)
  • Author(s): Nakayama Hisako、Abe Manabu、Morimoto Chie、Iida Tadatsune、Okabe Shigeo、Sakimura Kenji、Hashimoto Kouichi
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 2830-2830
  • DOI: 10.1038/s41467-018-05100-z
  • Peer Reviewed / Open Access
• [Journal Article] CAST/ELKS Proteins Control Voltage-Gated Ca2+ Channel Density and Synaptic Release Probability at a Mammalian Central Synapse (2018)
  • Author(s): Dong Wei、Radulovic Tamara、Goral R. Oliver、Thomas Connon、Suarez Montesinos Monica、Guerrero-Given Debbie、Hagiwara Akari、Putzke Travis、Hida Yamato、Abe Manabu、Sakimura Kenji、Kamasawa Naomi、Ohtsuka Toshihisa、Young Samuel M.
  • Journal Title: Cell Reports Volume: 24 Issue: 2 Pages: 284-293.e6
  • DOI: 10.1016/j.celrep.2018.06.024
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Targeting the SphK1/S1P/S1PR1 Axis That Links Obesity, Chronic Inflammation, and Breast Cancer Metastasis (2018)
  • Author(s): Nagahashi Masayuki、Yamada Akimitsu、Katsuta Eriko、Aoyagi Tomoyoshi、Huang Wei-Ching、Terracina Krista P.、Hait Nitai C.、Allegood Jeremy C.、Tsuchida Junko、Yuza Kizuki、Nakajima Masato、Abe Manabu、Sakimura Kenji、Milstien Sheldon、Wakai Toshifumi、Spiegel Sarah、Takabe Kazuaki
  • Journal Title: Cancer Research Volume: 78 Issue: 7 Pages: 1713-1725
  • DOI: 10.1158/0008-5472.can-17-1423
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CDC42EP4, a perisynaptic scaffold protein in Bergmann glia, is required for glutamatergic tripartite synapse configuration. (2018)
  • Author(s): Ageta-Ishihara N, Konno K, Yamazaki M, Abe M, Sakimura K, Watanabe M, Kinoshita M.
  • Journal Title: Neurochem Int Volume: S0197-0186 Issue: 17 Pages: 30442-4
  • DOI: 10.1016/j.neuint.2018.01.003
  • Peer Reviewed / Open Access
• [Journal Article] Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum (2018)
  • Author(s): Uesaka Naofumi、Abe Manabu、Konno Kohtarou、Yamazaki Maya、Sakoori Kazuto、Watanabe Takaki、Kao Tzu-Huei、Mikuni Takayasu、Watanabe Masahiko、Sakimura Kenji、Kano Masanobu
  • Journal Title: Neuron Volume: 97 Issue: 4 Pages: 796
  • DOI: 10.1016/j.neuron.2018.01.018
  • Peer Reviewed / Open Access
• [Journal Article] RNG105/Caprin1, an RNA granule protein for dendritic mRNA localization, is essential for long-term memory formation (2017)
  • Author(s): Kei Nakayama, Rie Ohashi, Yo Shinoda, Maya Yamazaki, Manabu Abe, Akihiro Fujikawa, Shuji Shigenobu, Akira Futatsugi, Masaharu Noda, Katsuhiko Mikoshiba, Teiichi Furuichi, Kenji Sakimura, and Nobuyuki Shiina
  • Journal Title: eLife Volume: 6 Pages: 1-32
  • DOI: 10.7554/elife.29677
  • Peer Reviewed / Open Access
• [Journal Article] Orexin modulates behavioral fear expression through the locus coeruleus (2017)
  • Author(s): Soya Shingo、Takahashi Tohru M.、McHugh Thomas J.、Maejima Takashi、Herlitze Stefan、Abe Manabu、Sakimura Kenji、Sakurai Takeshi
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1606-1606
  • DOI: 10.1038/s41467-017-01782-z
  • NAID: 120007128779
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency (2017)
  • Author(s): Miyamoto Hiroyuki、Shimohata Atsushi、Abe Manabu、Abe Teruo、Mazaki Emi、Amano Kenji、Suzuki Toshimitsu、Tatsukawa Tetsuya、Itohara Shigeyoshi、Sakimura Kenji、Yamakawa Kazuhiro
  • Journal Title: Human Molecular Genetics Volume: 26 Issue: 24 Pages: 4961-4974
  • DOI: 10.1093/hmg/ddx379
  • Peer Reviewed / Open Access
• [Journal Article] An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling (2017)
  • Author(s): Hayakawa-Yano Yoshika、Suyama Satoshi、Nogami Masahiro、Yugami Masato、Koya Ikuko、Furukawa Takako、Zhou Li、Abe Manabu、Sakimura Kenji、Takebayashi Hirohide、Nakanishi Atsushi、Okano Hideyuki、Yano Masato
  • Journal Title: Genes & Development Volume: 31 Issue: 18 Pages: 1910-1925
  • DOI: 10.1101/gad.300822.117
  • Peer Reviewed / Open Access
• [Journal Article] Netrin-1 Derived from the Ventricular Zone, but not the Floor Plate, Directs Hindbrain Commissural Axons to the Ventral Midline (2017)
  • Author(s): Yamauchi Kenta、Yamazaki Maya、Abe Manabu、Sakimura Kenji、Lickert Heiko、Kawasaki Takahiko、Murakami Fujio、Hirata Tatsumi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 11992-11992
  • DOI: 10.1038/s41598-017-12269-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1 (2017)
  • Author(s): Mashud Rana、Nomachi Akira、Hayakawa Akihide、Kubouchi Koji、Danno Sally、Hirata Takako、Matsuo Kazuhiko、Nakayama Takashi、Satoh Ryosuke、Sugiura Reiko、Abe Manabu、Sakimura Kenji、Wakana Shigeharu、Ohsaki Hiroyuki、Kamoshida Shingo、Mukai Hideyuki
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 7663-7663
  • DOI: 10.1038/s41598-017-07936-9
  • NAID: 120006366660
  • Peer Reviewed / Open Access
• [Journal Article] Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production (2017)
  • Author(s): Kawai Takafumi、Okochi Yoshifumi、Ozaki Tomohiko、Imura Yoshio、Koizumi Schuichi、Yamazaki Maya、Abe Manabu、Sakimura Kenji、Yamashita Toshihide、Okamura Yasushi
  • Journal Title: Journal of Neurochemistry Volume: 142 Issue: 5 Pages: 686-699
  • DOI: 10.1111/jnc.14106
  • Peer Reviewed / Open Access
• [Journal Article] The number and distribution of AMPA receptor channels containing fast kinetic GluA3 and GluA4 subunits at auditory nerve synapses depend on the target cells (2017)
  • Author(s): Rubio Mar?a E.、Matsui Ko、Fukazawa Yugo、Kamasawa Naomi、Harada Harumi、Itakura Makoto、Moln?r Elek、Abe Manabu、Sakimura Kenji、Shigemoto Ryuichi
  • Journal Title: Brain Structure and Function Volume: 222 Issue: 8 Pages: 3375-3393
  • DOI: 10.1007/s00429-017-1408-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The balance between Cathepsin C and Cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model. (2017)
  • Author(s): Shimizu T, Wisessmith W, Li J, Abe M, Sakimura K, Chetsawang B, Sahara Y, Tohyama K, Tanaka KF, Ikenaka K
  • Journal Title: Glia Volume: in press Issue: 6 Pages: 917-930
  • DOI: 10.1002/glia.23134
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 培養動物細胞におけるプライム編集によるノックイン効率改善の試み (2021)
  • Author(s): 阿部学
  • Organizer: 第44回分子生物学会年会
• [Presentation] 脊髄におけるBEGAIN陽性介在ニューロンの可視化 (2020)
  • Author(s): 片野泰代, 阿部学, 渡辺雅彦,﨑村建司, 伊藤誠二, 小林拓也
  • Organizer: 第43回日本神経科学大会
• [Presentation] 大脳基底核間接路のD2受容体発現細胞におけるNMDA受容体のマグネシウムブロックの減少により誘導される運動制御と学習記憶の変化の解析 (2020)
  • Author(s): 笹岡 俊邦, 齊藤 奈英, 知見聡美, 阿部 学, 川村 名子, 鍋島 陽一, 田井中 一貴, 﨑村 建司, 南部篤
  • Organizer: 第43回日本神経科学大会
• [Presentation] 異種間移植新生子精巣を用いた遺伝子改変ラット作製の効率化 (2020)
  • Author(s): 中務胞, 岩崎亜美, 夏目里恵, 阿部学, 崎村建司
  • Organizer: 第67回日本実験動物学会総会
• [Presentation] Aif1-iCre knock-in mouse line: a tool for conditional gene manipulation in microglia (2019)
  • Author(s): 阿部学
  • Organizer: 第42回日本神経科学大会
• [Presentation] ニューロンIDの可視化：クラスター型プロトカドヘリンの発現解析 (2018)
  • Author(s): 金子涼輔、阿部学、他6名
  • Organizer: 第41回 日本神経科学大会
  • Int'l Joint Research
• [Presentation] Aif1-iCre knock-in mouse line: a tool for conditional gene manipulation in microglia (2017)
  • Author(s): Abe Manabu, Fei Peng, Sakimura Kenji
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Remarks] 新潟大学脳研究所ホームページ > 研究分野紹介 > モデル動物開発分野
  • URL: https://www.bri.niigata-u.ac.jp/field/cellular/index.html
• [Remarks] 新潟大学脳研究所ホームページ > 研究分野紹介 > モデル動物開発分野
  • URL: https://www.bri.niigata-u.ac.jp/field/cellular/index.html
• [Remarks] 新潟大学脳研究所ホームページ > 研究分野紹介 > モデル動物開発分野
  • URL: http://www.bri.niigata-u.ac.jp/field/cellular/index.html
• [Remarks] 新潟大学脳研究所ホームページ ＞ 研究分野紹介 ＞ 細胞神経生物学分野
  • URL: http://www.bri.niigata-u.ac.jp/field/cellular/post.html
• [Patent(Industrial Property Rights)] Timothy症候群新規病態モデル (2021)
  • Inventor(s): 阿部学
  • Industrial Property Rights Holder: 国立大学法人新潟大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2021