Unravelling fundamental interactions underlying the rotation mechanism of F1-ATPase
| Project/Area Number |
17K05562
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Mathematical physics/Fundamental condensed matter physics
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| Research Institution | Tohoku University |
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Principal Investigator |
SASAKI Kazuo 東北大学, 工学研究科, 教授 (50205837)
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| Co-Investigator(Kenkyū-buntansha) |
吉留 崇 東北大学, 工学研究科, 助教 (90456830)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | F1-ATPase / 化学状態 / 相互作用ポテンシャル / ポアソン過程 / 統計解析 / 回転子・固定子相互作用 / 回転スリップ / タンパク質分子モーター / 拡散増大 / 統計力学 / 分子機械 / 生体分子 / １分子計測 |
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| Outline of Final Research Achievements |
The rotor of a protein motor F1-ATPase can be forcefully rotated by manipulating a magnetic field if a magnetic nanoparticle is attached to the rotor. An algorithm is proposed that can infer the fundamental interaction producing the rotational motion of this motor, by statistically analyzing a time course of the rotation angle of the rotor obtained experimentally. A similar algorithm proposed earlier failed to infer the interaction in certain rages of rotation angle. In contrast, our algorithm successfully inferred it in the whole rage of the angle in the analysis of time courses produced by computers based on a theoretical model of F1-ATPase.
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| Academic Significance and Societal Importance of the Research Achievements |
私たちの体の中でエネルギーを蓄える普遍的な分子であるATPの合成に関わるタンパク質F1-ATPaseは，回転の力学的エネルギーを化学エネルギー変換することでATPを合成する．本研究成果により，F1-ATPaseが高いエネルギー変換効率で効率的に働く仕組みを解明するために必要な物理学的情報を，実験データから獲得するための道が開かれた．
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Report
(4 results)
Research Products
(20 results)
