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Exploring novel pathogenic enteric viruses from selective metagenomic analysis of domestic wastewater

Research Project

Project/Area Number 17K06614
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Civil and environmental engineering
Research InstitutionThe University of Tokyo (2018-2019)
Ochanomizu University (2017)

Principal Investigator

Kazama Shinobu  東京大学, 大学院工学系研究科(工学部), 特任講師 (20749444)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords腸管系ウイルス / メタゲノム / 流入下水 / 未知塩基配列 / メタゲノム解析 / 下水 / 土木環境システム / ウイルス / 感染症 / ゲノム
Outline of Final Research Achievements

Using the selective metagenomic analysis method that efficiently detects single-stranded (+) RNA viruses, we succeeded in detecting a sequence considered to be an unknown viral genome in raw sewage and human stool samples. The sequence was detected in 100% of the raw sewage samples (31 samples) and 86% in the stool samples of gastroenteritis patients (50 samples). The method used in this study is expected to detect novel virus. It was also suggested that the sequence detected in this study is useful as a new indicator for fecal contamination.

Academic Significance and Societal Importance of the Research Achievements

途上国のみならず先進国においても,毎年ノロウイルス等の病原微生物による感染性胃腸炎が流行しており,我が国においてもその社会的損失は計り知れない。今後,社会の国際化や地球温暖化等による病原微生物の生態変化によって,新興ウイルスあるいは,これまで我が国では感染報告の無いウイルスによる新たな感染症の増加が懸念される。本研究では、新たなウイルスと考えられるゲノムの一部を下水から検出し、また地域における分布状況を示した。今後、本手法を応用することで、ウイルス感染症の発生状況を把握し,将来的なウイルス感染症の早期検出が期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017

All Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Presentation] 下水由来ss(+)RNAウイルスゲノム中の未知塩基配列の探索2019

    • Author(s)
      森山一葉,風間しのぶ,真砂佳史,大瀧雅寛
    • Organizer
      第53回 水環境学会年会
    • Related Report
      2019 Annual Research Report 2018 Research-status Report
  • [Presentation] 下水中のノロウイルスモニタリングによる胃腸炎監視システム2019

    • Author(s)
      風間しのぶ, 三浦尚之,真砂佳史,今田義光,当广謙太郎,真中太佳史,劉暁芳,伊藤絵里香,渡部徹,植木洋,甲野藤弘憲,斉藤繭子,押谷仁,大村達夫
    • Organizer
      ウイルス性下痢症研究会第31回学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Exploring unidentifiable RNA virus sequences from metagenomic analysis of domestic wastewater2019

    • Author(s)
      Shinobu Kazama, Hitoha Moriyama, Yoshifumi Masago, Masahiro Otaki
    • Organizer
      20th International Symposium on Health Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 下水由来(+)ssRNAウイルスのメタゲノム解析を用いた未知胃腸炎ウイルスの探索2018

    • Author(s)
      風間しのぶ、真砂佳史、森山一葉、大瀧雅寛
    • Organizer
      第5回環境水質工学シンポジウム
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] nvestigation of Unknown Genomes through Metagenomic Analysis of (+)ssRNA Viruses in Wastewater2018

    • Author(s)
      Hitoha Moriyama, Shinobu Kazama, Yoshifumi Masago, and Masahiro Otaki
    • Organizer
      the Water and Environment Technology Conference 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] 下水由来ss (+) RNAウイルスメタゲノム中の未知塩基配列の探索2017

    • Author(s)
      風間 しのぶ,真砂 佳史,三浦 尚之,今田 義光,井原 賢,田中 宏明,森山 一葉,大瀧 雅寛
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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