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Utilization of anaerobic spore bacteria as "functional" and "exhaustive" fecal contamination indicators and application to microbial risk assessment

Research Project

Project/Area Number 17K06620
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Civil and environmental engineering
Research InstitutionPrefectural University of Hiroshima

Principal Investigator

Hashimoto Atsushi  県立広島大学, 生命環境学部, 准教授 (30332068)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords嫌気性芽胞菌 / ウェルシュ菌 / 糞便汚染指標 / ソーストラッキング / MST / 汚染源探索指標 / PMMoV / 代替指標 / 大腸菌 / 腸球菌 / 河川流域管理
Outline of Final Research Achievements

Utilizing the ubiquity of anaerobic spore bacteria (C. perfringens) cpe gene-positive strains in human-derived fecal sources, which has been revealed in previous studies, to evaluate its effectiveness as a microbial source tracking indicator for human fecal pollution in river basins. In addition, the disinfection efficacy of peracetic acid against this spores was examined.
In field surveys on rivers receiving livestock point sources and human-derived point sources and non-point sources, the effectiveness of cpe-positive strains as MST and virus indicators was revealed. In addition, the disinfection experiment confirmed the disinfection effect of peracetic acid in the presence of organic substances.

Academic Significance and Societal Importance of the Research Achievements

大腸菌などの細菌が糞便汚染指標として用いられてきた。これらの指標細菌は、水などの糞便汚染の有無を評価する指標として機能してきているものの、人を含む様々な動物に由来する糞便汚染の識別、原虫やウイルス等を含む糞便-経口感染する微生物の指標としての効果は限定的である。本研究では、糞便汚染の汚染源識別指標(MST)指標としての「機能」性と原虫、ウイルスも含む「網羅」的な指標としての嫌気性芽胞菌(ウェルシュ菌)の意義を示した。これは、水道水源の微生物学的水質の評価を行う際に単なる糞便汚染の評価からより進歩した評価を行うことができるツールを示すものであり、微生物学的安全性の確保に有効に役立つものである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2018

All Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Presentation] 水中の細菌およびウイルスに対する過酢酸製剤の不活化効果2020

    • Author(s)
      鈴木裕之、藤田久美子、鶴巣和樹、橋本温
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Peracetic acid as an alternative disinfectant for chlorine-resistant microbes such as Clostridium perfringens spores in chlorinated effluent from sewage treatment plants2019

    • Author(s)
      Hiroyuki suzuki, Kenji Oonkaka and Atsushi Hashimoto
    • Organizer
      Watermicro 2019, 20th Symposium on Health Related Water Microbiology, IWA, Vienna
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 河川水中での糞便汚染指標細菌およびウイルスの挙動2018

    • Author(s)
      中本佳奈,野田司,岩本和也,橋本温
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 定量的微生物リスク評価を用いた水道水質管理の試み2018

    • Author(s)
      橋本温
    • Organizer
      日本防菌防黴学会 第45回年次大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 嫌気性芽胞菌のソーストラッキング指標およびウイルス指標としての特性2018

    • Author(s)
      中本佳奈、岩本和也、土岡宏彰、高田真那、橋本温
    • Organizer
      日本水環境学会第52回年会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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