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Functions of B4galt1 in the homing of hematopoietic stem cells.

Research Project

Project/Area Number 17K07135
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionKyoto University

Principal Investigator

Naruse Chie  京都大学, 医学研究科, 准教授 (30372486)

Co-Investigator(Kenkyū-buntansha) 浅野 雅秀  京都大学, 医学研究科, 教授 (50251450)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords造血幹細胞 / ホーミング / 糖鎖 / マウス / B4galt1 / 造血幹細胞分化 / β4GalT1 / beta4-GalT1 / GalT1 / 応用動物
Outline of Final Research Achievements

Colony formation assay and direct observation showed that β4-galactosyltransferase-1 knockout (β4GalT-1-/-) bone marrow-derived hematopoietic stem cells could not engraft in recipient mice. In addition, as a result of analyzing whether or not fetal β4GalT1-/- hematopoietic stem cells engraft in wild-type mice, it was found that fetal β4GalT1-/- hematopoietic stem cells cannot engraft after transplantation as in adults. Furthermore, the number of pluripotent progenitor cells was slightly increased in β4GalT-1-/- bone marrow cells, but the number of hematopoietic stem cells was not different from that of wild type. Therefore, β4GalT-1 was considered to be essential for homing, not related to the number and proliferative capacity of hematopoietic stem cells.

Academic Significance and Societal Importance of the Research Achievements

造血幹細胞が骨髄にホーミングするためにはβ4GalT-1によって合成されるN型糖鎖が必須であることが明らかになった。臍帯血の造血幹細胞は,成人骨髄中の造血幹細胞と比較すると糖鎖修飾に違いがあることが報告されているので,これが臍帯血に含まれる造血幹細胞の低いホーミング活性が糖鎖修飾の違いによるものだとすれば,糖鎖修飾技術を応用して臍帯血に含まれる造血幹細胞のホーミング活性を増強することで,移植効率の飛躍的向上をもたらす方法の開発につながる可能性を示唆した。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow2019

    • Author(s)
      Takagaki Soichiro、Yamashita Rieko、Hashimoto Noriyoshi、Sugihara Kazushi、Kanari Kanako、Tabata Keisuke、Nishie Toshikazu、Oka Shogo、Miyanishi Masanori、Naruse Chie、Asano Masahide
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 7133-7133

    • DOI

      10.1038/s41598-019-43551-6

    • NAID

      120006629179

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow.2019

    • Author(s)
      Soichiro Takagaki, Rieko Yamashita, Noriyoshi Hashimoto, Kazushi Sugihara, Kanako Kanari, Keisuke Tabata, Toshikazu Nishie, Shogo Oka, Masanori Miyanishi, Chie Naruse, and Masahide Asano
    • Journal Title

      Scientific Reports

      Volume: -

    • NAID

      120006629179

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 造血幹細胞のホーミングおよび分化におけるB4Galt1の役割2019

    • Author(s)
      山下莉映子,成瀬智恵,杉原一司,金成香奈子,高垣聡一郎,宮西正憲,岡昌吾,浅野雅秀
    • Organizer
      第66回日本実験動物学会総会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 京都大学大学院医学研究科 実験動物学分野研究室

    • URL

      http://www.anim.med.kyoto-u.ac.jp/research/index.html

    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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