The mechanism for severging of F-actin by gelsolin-like protein
| Project/Area Number |
17K07373
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokai Gakuin University |
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Principal Investigator |
Oda Toshiro 東海学院大学, 健康福祉学部, 教授(移行) (20321739)
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| Co-Investigator(Kenkyū-buntansha) |
藤原 郁子 名古屋工業大学, 工学(系)研究科(研究院), 助教 (10742075)
武田 修一 名古屋大学, 理学研究科, 研究員 (50509081)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | アクチン / フラグミン / 構造空間 / 1分子 / ゲルゾリン / 動力学計算 / TIRF / 生物物理 / 一分子測定 / X線構造解析 |
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| Outline of Final Research Achievements |
Actin plays an important role in various cell functions by converting between monomeric and filamentous states. In this study, in order to understand how actin functions, we defined the conformational space for available structure of actin and showed existing of four clusters within the space (G-form, F-form, C-form and O-form). In addition, we revealed transitions between four cluster structures. We also studied the structure of fragmin, which regulates actin function.
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| Academic Significance and Societal Importance of the Research Achievements |
蛋白質は構造を変化させながら機能している。そのため、蛋白質の機能プロセスを理解するためには構造転移を解明する必要がある。この考えをアクチンに適用してアクチンの構造空間を定義した。この空間は研究者がアクチンの構造転移を議論する際の共通基盤となる。また、本研究で得られたゲルゾリン・ファミリーに属するフラグミン対する知見は、ゲルゾリンの基礎研究やその医療応用に貢献することが期待される。ゲルゾリンは医学の見た場合非常に重要な蛋白質で、例えばその欠乏が敗血症を引き起こす。
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Report
(4 results)
Research Products
(33 results)
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[Presentation] “Bacterial actin homolog, MreBs and fibril are essential for the swimming motility of Spiroplasma eriocheiris”,2019
Author(s)
Fujiwara, I., Takahashi, D., Sasajima, Y., Kiyama, H., Arakawa, A., Kato, K., Miyata, T., Kakizawa, S., Namba, K. and Miyata, M.
Organizer
The 11th Toyota Riken International Workshop"Actin Filament: beyond the atomic resolution structures"
Related Report
Int'l Joint Research / Invited
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[Presentation] Cell motility based on the polymerization dynamics”2019
Author(s)
Fujiwara, I., Takahashi, D., Sasajima, Y., Remmert, K., Hammer, J. and Miyata, M.
Organizer
International Workshop on Quantum Sensing & Biophotonics
Related Report
Int'l Joint Research
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[Presentation] Atomic resolution structures of F‐form actin: mutual switching between G/F transition and ATPase2018
Author(s)
Shuichi Takeda, Akihiro Narita, Toshiro Oda, Nobuhisa Watanabe, Takayuki Nagae, Motonori Ota, Ryotaro Koike, Kei Moritsugu, Yusuke Kanematsu, Ikuko Fujiwara, Kotaro Tanaka, Yuichiro Maéda
Organizer
The 47th European muscle conference
Related Report
Int'l Joint Research
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[Presentation] The structure of F-form actin at atomic resolutions: mutual switching between polymorphic transition and ATPase2018
Author(s)
Shuichi Takeda, Akihiro Narita, Toshiro Oda, Nobuhisa Watanabe, Y, Takayuki Nagae, Motonori Ota, Ryotaro Koike, Kei Moritsugu, Yusuke Kanematsu, Ikuko Fujiwara, Kotaro Tanaka, Yuichiro Maéda
Organizer
Myofilament conference 2018
Related Report
Int'l Joint Research
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[Presentation] Severing regulation of gelsolin superfamily on single and bundled actin filaments2017
Author(s)
Fujiwara,I., Fan,R., Takeda,S., Maéda,Y., Narita, A
Organizer
International Symposium, Harmonized supramolecular motility machinery and its diversity, Nagoya, Japan 09/2017
Related Report
Int'l Joint Research
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