| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Intracellular vesicles and organelles are transported within a cell along both microtubules and actin filaments bidirectionally, which requires spatial-temporal regulation of cytoskeletons and switching molecular motors. However molecular basis of such regulation is poorly understood. In this study, we tried to answer this issue by accurate measuring the number of molecular motors responsible for vesicle transport and manipulating molecular motor localization using fish and frog melanophore as a model system of vesicle transport. We showed our newly developed method revealed several force producing dyneins associated each melanosome and showed cooperative movement. Our findings indicated the regulation of the number of force producing motor plays essential role in intracellular transport.
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