Analysis of the neural reorganization by a sugar-responsive hormone, CCHa2.

• Project/Area Number: 17K07419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kurume University
• Principal Investigator: SANO Hiroko 久留米大学, 付置研究所, 講師 (90506908)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ホルモン / 栄養応答 / 遺伝子発現 / 糖代謝 / 求愛行動 / 神経回路 / インスリン / CCHa2 / 糖依存的転写 / ポリオール経路 / 発生・分化 / 神経科学 / 行動学 / 昆虫

Outline of Final Research Achievements

In order to clarify the mechanisms of neural reorganization by nutritional conditions, we analyzed how a sugar-responsive hormone, CCHa2, regulates the formation of Fruitless neurons that are required for courtship behavior of adult male flies. We have shown that glucose ingestion is transmitted to a transcription factor, Mondo, through the polyol pathway resulting in the expression of CCHa2. CCHa2 then promotes insulin secretion, which in turn activates Fruitless neurons. We have further shown that appropriate level of insulin signaling is required for proper function of Fruitless neurons.

Academic Significance and Societal Importance of the Research Achievements

環境に応じた神経回路の組みかえは行動の変化に直結することから、そのメカニズムの解明は動物が環境に適応するための仕組みや、環境要因と遺伝要因の組み合わせによる個体差すなわち個性がどのように生じるのかを理解することに役立つと考えられる。また、環境が関与すると考えられている精神疾患の発症メカニズムの解明にも道を開く可能性がある。

Research Products

• [Journal Article] Ex vivo calcium imaging for visualizing brain responses to endocrine signaling in Drosophila. (2018)
  • Author(s): Ishimoto, H. and Sano, H.
  • Journal Title: Journal of Visualized Experiments Volume: 未定
  • Peer Reviewed
• [Presentation] The polyol pathway is crucial for glucose sensing and sugar-responsive changes in metabolic gene expression (2019)
  • Author(s): Hiroko Sano, Akira Nakamura, Mariko Yamane, Hitoshi Niwa, Takashi Nishimura, and Masayasu Kojima
  • Organizer: 第52回日本発生生物学会年会
• [Presentation] 個体はどのようにして糖摂取を感知するのか？: ショウジョウバエおよびマウスモデルを用いた遺伝学的解析 (2019)
  • Author(s): 佐野浩子
  • Organizer: 第7回TOR研究会
• [Presentation] The role of the polyol pathway in Mondo-mediated maintenance of metabolic homeostasis in Drosophila melanogaster (2019)
  • Author(s): Hiroko Sano, Akira Nakamura, Mariko Yamane, Hitoshi Niwa, Kimi Araki, Kazumasa Takemoto, Kei-ichiro Ishiguro, Yuki Takada, Takashi Nishimura, and Masayasu Kojima
  • Organizer: 2019 Insect Hormone Workshop
  • Int'l Joint Research
• [Presentation] The polyol pathway is the principal sugar-sensing pathway for sugar-responsive gene expression mediated by Mondo/ChREBP. (2018)
  • Author(s): H. Sano, A. Nakamura, M. Yamane, H. Niwa and M. Kojima
  • Organizer: 第70回日本細胞生物学会第51回日本発生生物学会合同大会
• [Presentation] The polyol pathway is the principal sugar-sensing pathway for Mondo/ChREBP-mediated transcription (2018)
  • Author(s): 佐野浩子
  • Organizer: CDB Symposium 2018
  • Int'l Joint Research
• [Presentation] Mechanisms for the sugar-dependent expression of the CCHa2 peptide and its roles in Drosophila physiology and behavior. (2017)
  • Author(s): 佐野浩子
  • Organizer: The 3rd Insect Hormone Workshop
  • Int'l Joint Research
• [Remarks] Researchmap link
  • URL: https://researchmap.jp/sapphire