Single cell analysis of plasticity of mouse sperm stem cells
| Project/Area Number |
17K07424
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 幹細胞 / 精子 / 生殖細胞 / シングルセル解析 / マウス / 精子幹細胞 / 精巣 / Eomes / single cell analysis / multiplex qRT-PCR / 哺乳類 / 発生・分化 |
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| Outline of Final Research Achievements |
The purpose of this study is to reveal the molecular process of reversion from potential stem cells, primed for differentiation, to actual stem cells, actually self-renew in steady-state condition using single-cell transcriptome analysis. Based on analysis by Fluidigm C1 and Biomark HD system and recent reports, T-box transcription factor Eomes is one of the candidate gene involving the reversion process. For now, apparent effects of overexpression of Eomes on gene expression in cultured sperm stem cells and its loss of function on stem cell number under the physiological condition have not been observed, and therefore investigation of the effect under the regeneration process will be needed.
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| Academic Significance and Societal Importance of the Research Achievements |
マウスの精子幹細胞は、絶え間なく精子を作り続ける。精子幹細胞を含む生殖細胞が傷害された後に起こる再生時には、本来分化するはずだった細胞が、その運命を転換することで再生が促進される。しかし、どのようなプログラムが働くのか不明であった。今回、それに関与する候補の遺伝子を選定し、その機能解析を行った。本研究は、将来的にどのような分子メカニズムで幹細胞集団が元に戻るのかと言う問題を解く糸口になる可能性がある。
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Report
(5 results)
Research Products
(7 results)
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[Journal Article] EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice2021
Author(s)
Y. Osawa, M. Usui, Y. Kuba, H.T. Le, N. Mikami, T. Nakagawa, Y. Daitoku, K. Kato, H.H. Shawki, Y. Ikeda, A. Kuno, K. Morimoto, Y. Tanimoto, T.T.H. Dinh, K. Murata, K. Yagami, M. Ema, S. Yoshida, S. Takahashi, S. Mizuno, and F. Sugiyama
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Journal Title
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche2019
Author(s)
Kitadate Y., Jorg DJ., Tokue M,. Maruyama A., Ishikawa R., Tsuchiya S., Segi-Nishida E., Nakagawa T., Uchida A., Kimura-Yoshida C., Mizuno S., Sugiyama F., Azami T., Ema M., Noda C., Kobayashi S., Matsuo I., Kanai Y., Nagasawa T., Sugimoto Y., Takahashi S., Simons BD., Yoshida S.
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Journal Title
Cell Stem Cell
Volume: 24
Issue: 1
Pages: 79-92
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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