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Single cell analysis of plasticity of mouse sperm stem cells

Research Project

Project/Area Number 17K07424
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Toshinori Nakagawa  基礎生物学研究所, 生殖細胞研究部門, 助教 (50456894)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords幹細胞 / 精子 / 生殖細胞 / シングルセル解析 / マウス / 精子幹細胞 / 精巣 / Eomes / single cell analysis / multiplex qRT-PCR / 哺乳類 / 発生・分化
Outline of Final Research Achievements

The purpose of this study is to reveal the molecular process of reversion from potential stem cells, primed for differentiation, to actual stem cells, actually self-renew in steady-state condition using single-cell transcriptome analysis. Based on analysis by Fluidigm C1 and Biomark HD system and recent reports, T-box transcription factor Eomes is one of the candidate gene involving the reversion process. For now, apparent effects of overexpression of Eomes on gene expression in cultured sperm stem cells and its loss of function on stem cell number under the physiological condition have not been observed, and therefore investigation of the effect under the regeneration process will be needed.

Academic Significance and Societal Importance of the Research Achievements

マウスの精子幹細胞は、絶え間なく精子を作り続ける。精子幹細胞を含む生殖細胞が傷害された後に起こる再生時には、本来分化するはずだった細胞が、その運命を転換することで再生が促進される。しかし、どのようなプログラムが働くのか不明であった。今回、それに関与する候補の遺伝子を選定し、その機能解析を行った。本研究は、将来的にどのような分子メカニズムで幹細胞集団が元に戻るのかと言う問題を解く糸口になる可能性がある。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye2021

    • Author(s)
      Y.H. Yeh, M. Hu, T. Nakagawa, A. Sakashita, S. Yoshida, S. Maezawa and S.H. Namekaw
    • Journal Title

      J Vis Exp

      Volume: - Issue: 167

    • DOI

      10.3791/61666

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice2021

    • Author(s)
      Y. Osawa, M. Usui, Y. Kuba, H.T. Le, N. Mikami, T. Nakagawa, Y. Daitoku, K. Kato, H.H. Shawki, Y. Ikeda, A. Kuno, K. Morimoto, Y. Tanimoto, T.T.H. Dinh, K. Murata, K. Yagami, M. Ema, S. Yoshida, S. Takahashi, S. Mizuno, and F. Sugiyama
    • Journal Title

      eLife

      Volume: -

    • NAID

      120007190954

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche2019

    • Author(s)
      Kitadate Y., Jorg DJ., Tokue M,. Maruyama A., Ishikawa R., Tsuchiya S., Segi-Nishida E., Nakagawa T., Uchida A., Kimura-Yoshida C., Mizuno S., Sugiyama F., Azami T., Ema M., Noda C., Kobayashi S., Matsuo I., Kanai Y., Nagasawa T., Sugimoto Y., Takahashi S., Simons BD., Yoshida S.
    • Journal Title

      Cell Stem Cell

      Volume: 24 Issue: 1 Pages: 79-92

    • DOI

      10.1016/j.stem.2018.11.013

    • NAID

      120007128265

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Quantitative flux analysis of multistate spermatogenic stem cells in homeostasis2020

    • Author(s)
      Toshinori Nakagawa, David J. Jorg, Benjamin D. Simons, Takashi Nagasawa, and Shosei Yoshida
    • Organizer
      Cold Spring Harbor Laboratory (Germ Cells)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Plvap expression revealed that regular division and stochastic state transition coordinate the maintenance of mouse spermatogenic stem cell pool2019

    • Author(s)
      Toshinori Nakagawa
    • Organizer
      第17回幹細胞シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] Mouse spermatogenic stem cells interconvert between different states at the top of differentiation hierarchy2018

    • Author(s)
      Toshinori Nakagawa, David Jorg, Ben Simons, Takashi Nagasawa, Shosei Yoshida
    • Organizer
      Cold Spring Harbor meeting: Germ Cells
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Spermatogenic stem cells interconvert between different states at the top of differentiation hierarchy2018

    • Author(s)
      Toshinori Nakagawa, David Jorg, Ben Simons, Takashi Nagasawa, Shosei Yoshida
    • Organizer
      日本発生生物学会 秋季シンポジウム 2018
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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