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Transmission mechanism of positional information to living tissue by fluid flow.

Research Project

Project/Area Number 17K07425
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionCenter for Novel Science Initatives, National Institutes of Natural Sciences (2018-2020)
National Institute for Basic Biology (2017)

Principal Investigator

Nonaka Shigenori  大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 准教授 (90435529)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords左右性 / 繊毛 / ライトシート顕微鏡 / 左右性決定 / 発生学 / 発生 / 水流 / 左右非対称性 / 発生・分化 / 生物物理 / 生理学
Outline of Final Research Achievements

In mammalian development, fluid flow on the surface of embryo generated by ciliary movement governs left-right determination, whereas the sensing mechanism of the flow remain enigmatic. We tested several hypotheses for this mechanism, and found that the mechanosensory model, the most influential one, is unlikely to work as its original form. The other ones we did not come to the conclusion and will continue further analyses.

Academic Significance and Societal Importance of the Research Achievements

本研究は、生物物理学的には、生体内において水流がその向きだけでなく上流・下流という位置情報を伝える、他にあまり例のないユニークなシステムの解析である。発生学的には、体軸決定という最も基本的な問題に迫るものである。医学的には、ヒトにも稀に見られる内臓逆位症の原因を理解するためのものである。結果、完全な解明には至らなかったが、今まで最有力視されてきた仮説について、少なくとも提唱されているそのままのかたちでは成立しないであろうという知見を得ることができた。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (33 results)

All 2020 2019 2018 2017 Other

All Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 12 results,  Open Access: 12 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Int'l Joint Research: 3 results,  Invited: 8 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Light-sheet microscopy-based 3D single-cell tracking reveals a correlation between cell cycle and the start of endoderm cell internalization in early zebrafish development2020

    • Author(s)
      Kondow Akiko、Ohnuma Kiyoshi、Kamei Yasuhiro、Taniguchi Atsushi、Bise Ryoma、Sato Yoichi、Yamaguchi Hisateru、Nonaka Shigenori、Hashimoto Keiichiro
    • Journal Title

      Development, Growth & Differentiation

      Volume: 62 Issue: 7-8 Pages: 495

    • DOI

      10.1111/dgd.12695

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transient microglial absence assists postmigratory cortical neurons in proper differentiation2020

    • Author(s)
      Yuki Hattori, Yu Naito, Yoji Tsugawa, Shigenori Nonaka, Hiroaki Wake, Takashi Nagasawa, Takaki Miyata
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 1631-1631

    • DOI

      10.1038/s41467-020-15409-3

    • NAID

      120006841707

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers2019

    • Author(s)
      Serizawa Takashi、Isotani Ayako、Matsumura Takafumi、Nakanishi Katsuyuki、Nonaka Shigenori、Shibata Shinsuke、Ikawa Masahito、Okano Hideyuki
    • Journal Title

      Development

      Volume: 146 Pages: 1-11

    • DOI

      10.1242/dev.174938

    • NAID

      120006957441

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] System level analysis of motor-related neural activities in larval Drosophila2019

    • Author(s)
      Yoon Youngteak、Park Jeonghyuk、Taniguchi Atsushi、Kohsaka Hiroshi、Nakae Ken、Nonaka Shigenori、Ishii Shin、Nose Akinao
    • Journal Title

      Journal of Neurogenetics

      Volume: 33 Issue: 3 Pages: 179-189

    • DOI

      10.1080/01677063.2019.1605365

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Calaxin is required for cilia-driven determination of vertebrate laterality2019

    • Author(s)
      Sasaki Keita、Shiba Kogiku、Nakamura Akihiro、Kawano Natsuko、Satouh Yuhkoh、Yamaguchi Hiroshi、Morikawa Motohiro、Shibata Daisuke、Yanase Ryuji、Jokura Kei、Nomura Mami、Miyado Mami、Takada Shuji、Ueno Hironori、Nonaka Shigenori、Baba Tadashi、Ikawa Masahito、Kikkawa Masahide、Miyado Kenji、Inaba Kazuo
    • Journal Title

      Communications Biology

      Volume: 2 Issue: 1 Pages: 226-226

    • DOI

      10.1038/s42003-019-0462-y

    • NAID

      120006957456

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolutionary transformation of mouthparts from particle-feeding to piercing carnivory in Viper copepods: Review and?3D analyses of a key innovation using advanced imaging techniques2019

    • Author(s)
      Kaji Tomonari、Song Chihong、Murata Kazuyoshi、Nonaka Shigenori、Ogawa Kota、Kondo Yusuke、Ohtsuka Susumu、Palmer A. Richard
    • Journal Title

      Frontiers in Zoology

      Volume: 16 Issue: 1

    • DOI

      10.1186/s12983-019-0308-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Skeleton construction upon local regression of the sponge body2019

    • Author(s)
      Kishimoto Kouji、Sugano‐Yasunaga Wakana、Taniguchi Atsushi、Agata Kiyokazu、Nonaka Shigenori、Funayama Noriko
    • Journal Title

      Development, Growth & Differentiation

      Volume: 61 Issue: 9 Pages: 485-500

    • DOI

      10.1111/dgd.12636

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Influence of cellular shape on sliding behavior of ciliates2018

    • Author(s)
      Nishigami Yukinori、Ohmura Takuya、Taniguchi Atsushi、Nonaka Shigenori、Manabe Junichi、Ishikawa Takuji、Ichikawa Masatoshi
    • Journal Title

      Communicative & Integrative Biology

      Volume: 11 Issue: 4 Pages: e1506666-e1506666

    • DOI

      10.1080/19420889.2018.1506666

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simple mechanosense and response of cilia motion reveal the intrinsic habits of ciliates2018

    • Author(s)
      Ohmura Takuya, Nishigami Yukinori, Taniguchi Atsushi, Nonaka Shigenori, Manabe Junichi, Ishikawa Takuji, Ichikawa Masatoshi
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 115 Issue: 13 Pages: 3231-3236

    • DOI

      10.1073/pnas.1718294115

    • NAID

      120006416185

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rotation of stress fibers as a single wheel in migrating fish keratocytes2018

    • Author(s)
      Okimura, C., Taniguchi, A., Nonaka, S. and Iwadate, Y.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 10615-10615

    • DOI

      10.1038/s41598-018-28875-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Axially-confined in vivo single-cell labeling by primed conversion using blue and red lasers with conventional confocal microscopes2017

    • Author(s)
      Taniguchi Atsushi、Kimura Yukiko、Mori Ikue、Nonaka Shigenori、Higashijima Shin-ichi
    • Journal Title

      Development, Growth & Differentiation

      Volume: 59 Issue: 9 Pages: 741-748

    • DOI

      10.1111/dgd.12412

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Live imaging of primary ocular vasculature formation in zebrafish2017

    • Author(s)
      Hashiura T, Kimura E, Fujisawa S, Oikawa S, Nonaka S, Kurosaka D, Hitomi J
    • Journal Title

      PLoS One

      Volume: 12(4) Issue: 4 Pages: e0176456-e0176456

    • DOI

      10.1371/journal.pone.0176456

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] マウス胚ノード繊毛の基底小体の左右性2020

    • Author(s)
      餘家博、谷口篤史、野中茂紀
    • Organizer
      第53回発生生物学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高速ライトシート顕微鏡の開発及びそれを用いた発生の左右初期決定機構の解析2020

    • Author(s)
      野中茂紀
    • Organizer
      レーザー学会学術講演会第40回年次大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 哺乳類発生における左右非対称性の初期決定2019

    • Author(s)
      野中茂紀
    • Organizer
      弘前大学農学生命科学部研究推進セミナー
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 光シート顕微鏡の原理と近年の動向2019

    • Author(s)
      野中茂紀
    • Organizer
      日本学術振興会ナノプローブテクノロジー第167委員会第91回研究会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 三次元粒子画像流速測定法によるノード流の解析2019

    • Author(s)
      谷口篤史、西上幸範、野中茂紀
    • Organizer
      第57回年日本生物物理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Analysis of nodal flow by three-dimensional particle image velocimetry2019

    • Author(s)
      Atsushi Taniguchi, Yukinori Nishigami, and Shigenori Nonaka
    • Organizer
      The 2nd NIBB-Princeton Joint Symposium "Imaging and Quantitative Biology"
    • Related Report
      2019 Research-status Report
  • [Presentation] 三次元粒子画像流速測定法によるノード流の解析2019

    • Author(s)
      谷口篤史、西上幸範、野中茂紀
    • Organizer
      第2回ExCELLSシンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] Study on left-right bias in arrangements of basal body-associated proteins in mouse node cilia2019

    • Author(s)
      Hiroshi Yoke and Shigenori Nonaka
    • Organizer
      The 2nd NIBB-Princeton Joint Symposium "Imaging and Quantitative Biology"
    • Related Report
      2019 Research-status Report
  • [Presentation] Study on left-right bias in arrangements of basal body-associated proteins in mouse node cilia2019

    • Author(s)
      餘家博、野中茂紀
    • Organizer
      第2回ExCELLSシンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] マウス胚ノード繊毛基底小体の左右性2019

    • Author(s)
      餘家博、野中茂紀
    • Organizer
      第10回繊毛研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Analysis of initial determination of left-right asymmetry in development using fast light-sheet microscopy2019

    • Author(s)
      野中茂紀
    • Organizer
      新学術領域レゾナンスバイオ国際支援共催セミナー
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 高速ライトシート顕微鏡の開発及びそれを用いた発生の左右初期決定機構の解析2019

    • Author(s)
      野中茂紀
    • Organizer
      第25回医用近赤外線分光法研究会・2018年度分光学会生細胞分光部会・第7回レーザー学会ニューロフォトニクス研究会 合同研究会 "Medical Optics & Spectroscopy 2018"
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Initial determination of left-right asymmetry in mammalian development2019

    • Author(s)
      Shigenori Nonaka
    • Organizer
      ExCELLS Frontier Bioorganization Forum
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] マルチ顕微鏡活用術2017

    • Author(s)
      野中茂紀
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Three-dimensional analysis of the nodal flow in the mouse embryo by the rapid 3-D light-sheet microscopy2017

    • Author(s)
      Atsushi Taniguchi, Shigenori Nonaka
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ライトシート・ライトフィールド顕微鏡が実現する細胞・組織レベルの超高速3Dイメージング2017

    • Author(s)
      野中茂紀
    • Organizer
      バイオナノフォトニクス新産業創造研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] 実験医学2019年8月号記事「イメージングデータ解析よろず相談所 大量画像解析のための自動化手法・基礎編」2019

    • Author(s)
      野中茂紀
    • Total Pages
      7
    • Publisher
      羊土社
    • ISBN
      9784758125222
    • Related Report
      2019 Research-status Report
  • [Book] イメージングの選び方・使い方100+(3457-3459 「光シート顕微鏡の動向」を執筆)2018

    • Author(s)
      原田慶恵、永井健治、野中茂紀、他
    • Total Pages
      236
    • Publisher
      羊土社
    • ISBN
      9784758103756
    • Related Report
      2018 Research-status Report
  • [Book] ライフサイエンス顕微鏡学ハンドブック2018

    • Author(s)
      山科正平、高田邦昭 責任編集(野中茂紀は「組織の観察」を担当)
    • Total Pages
      344
    • Publisher
      朝倉書店
    • ISBN
      9784254310948
    • Related Report
      2017 Research-status Report
  • [Remarks] Laboratory for Spatiotemporal Regulations

    • URL

      http://www.nibb.ac.jp/~bioimg2/index.html

    • Related Report
      2017 Research-status Report
  • [Remarks] 簡単な物理モデルで解き明かす微生物の生存戦略~繊毛虫テトラヒメナの壁面付近への集積メカニズムを解明~

    • URL

      http://www.nibb.ac.jp/pressroom/news/2018/03/15.html

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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