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Study on nitrogen depletion signaling in plants

Research Project

Project/Area Number 17K07438
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Imamura Sousuke  東京工業大学, 科学技術創成研究院, 准教授 (70548122)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords窒素代謝 / 窒素欠乏 / シグナル伝達 / 微細藻類
Outline of Final Research Achievements

Nitrogen is an important nutrient for plants since whose content determines their growth. However, despite the importance, there are many points to be solved for the control mechanism of nitrogen metabolism in the plant lineages. The purpose of this study is to clarify how the plant senses the nitrogen signal and transmits it to lead to the final responses, such as activation of transcription. In this study, we found that (1)components of TOR complex are changed depending on the nitrogen status, and (2) MYB1, the nitrogen depletion responsive transcription factor, has domains that control the activity of MYB1 positively and negatively. It was also revealed that the negative domain affects DNA binding activity and intracellular localization of MYB1.

Academic Significance and Societal Importance of the Research Achievements

窒素は、植物の生長を左右する重要な栄養元素である。窒素に関する研究としては、窒素量の増減に応じた遺伝子発現など、窒素量変化に伴う最終的な応答反応については良く理解されている。その一方で、窒素量の変化を、植物細胞がどの様な機構で感知しているのか、という点については、多くの制御系・実験系が複雑であるなどの理由で解明がなされていない。本研究では、植物が窒素欠乏を受容してから最終的な応答を引き起こす機構の一端を明らかにした。この明らかにされた機構を更に詳しく解明し、その応答反応を人為的に調節することにより、植物の窒素要求量を減らしたり、植物の成長を促進させるなどの応用研究に資することが可能になる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (46 results)

All 2020 2019 2018 2017 Other

All Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 5 results) Presentation (28 results) (of which Int'l Joint Research: 7 results,  Invited: 5 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Microalgal Target of Rapamycin (TOR): A Central Regulatory Hub for Growth, Stress Response and Biomass Production2020

    • Author(s)
      Pancha Imran、Chokshi Kaumeel、Tanaka Kan、Imamura Sousuke
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 4 Pages: 675-684

    • DOI

      10.1093/pcp/pcaa023

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification and analysis of a principal sigma factor interacting protein SinA, essential for growth at high temperatures in a cyanobacterium <i>Synechococcus elongatus</i> PCC 79422020

    • Author(s)
      Hazuki Hasegawa, Tatsuhiro Tsurumaki, Ikki Kobayashi, Sousuke Imamura and Kan Tanaka
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 66 Issue: 2 Pages: 66-72

    • DOI

      10.2323/jgam.2019.05.002

    • NAID

      130007855492

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overexpression of a glycogenin, CmGLG2, enhances floridean starch accumulation in the red alga Cyanidioschyzon merolae2019

    • Author(s)
      Imran Pancha, Kan Tanaka and Sousuke Imamura
    • Journal Title

      Plant Signal. Behav.

      Volume: 14 Issue: 6 Pages: 1596718-1596718

    • DOI

      10.1080/15592324.2019.1596718

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple modification of chromosomal loci using URA5.3 selection marker in the unicellular red alga Cyanidioschyzon merolae.2019

    • Author(s)
      Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka
    • Journal Title

      Bio-protocol

      Volume: 9 Issue: 7

    • DOI

      10.21769/bioprotoc.3204

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] オイル生産性が飛躍的に向上した藻類株の作出:オイル生合成のチェックポイントキナーゼTORの発見とその応用2019

    • Author(s)
      今村 壮輔、田中 寛
    • Journal Title

      BIO INDUSTRY

      Volume: 36 Pages: 9-19

    • Related Report
      2019 Annual Research Report
  • [Journal Article] オイル生産性を飛躍的に高めた藻類株の作出ー 藻類オイル生合成制御の理解とその応用ー2019

    • Author(s)
      今村 壮輔、田中 寛
    • Journal Title

      配管技術

      Volume: 61 Pages: 18-22

    • Related Report
      2019 Annual Research Report
  • [Journal Article] オイル生産性が飛躍的に向上した藻類株の創出ー藻類オイル生合成制御の理解とその応用ー2019

    • Author(s)
      今村 壮輔、田中 寛
    • Journal Title

      クリーンエネルギー

      Volume: 28 Pages: 1-6

    • NAID

      40021799524

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Top Starch Plating Method for the Efficient Cultivation of Unicellular Red Alga Cyanidioschyzon merolae2019

    • Author(s)
      Takemura Tokiaki、Kobayashi Yuki、Imamura Sousuke、Tanaka Kan
    • Journal Title

      BIO-PROTOCOL

      Volume: 9 Issue: 4

    • DOI

      10.21769/bioprotoc.3172

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Target of rapamycin-signaling modulates starch accumulation via glycogenin phosphorylation status in the unicellular red alga Cyanidioschyzon merolae2018

    • Author(s)
      Pancha Imran、Shima Hiroki、Higashitani Nahoko、Igarashi Kazuhiko、Higashitani Atsushi、Tanaka Kan、Imamura Sousuke
    • Journal Title

      The Plant Journal

      Volume: 97 Issue: 3 Pages: 485-499

    • DOI

      10.1111/tpj.14136

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae2018

    • Author(s)
      Fukuda Satoshi、Hirasawa Eri、Takemura Tokiaki、Takahashi Sota、Chokshi Kaumeel、Pancha Imran、Tanaka Kan、Imamura Sousuke
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 12410-12410

    • DOI

      10.1038/s41598-018-30809-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Construction of a Selectable Marker Recycling System and the Use in Epitope Tagging of Multiple Nuclear Genes in the Unicellular Red Alga Cyanidioschyzon merolae2018

    • Author(s)
      Takemura Tokiaki、Imamura Sousuke、Kobayashi Yuki、Tanaka Kan
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Pages: 2308-2316

    • DOI

      10.1093/pcp/pcy156

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The checkpoint kinase TOR (target of rapamycin) regulates expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) and modulates chloroplast ribosomal RNA synthesis in a unicellular red alga2018

    • Author(s)
      Imamura Sousuke、Nomura Yuhta、Takemura Tokiaki、Pancha Imran、Taki Keiko、Toguchi Kazuki、Tozawa Yuzuru、Tanaka Kan
    • Journal Title

      Plant J

      Volume: 94 Issue: 2 Pages: 327-339

    • DOI

      10.1111/tpj.13859

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Construction of a rapamycin-susceptible strain of the unicellular red alga <i>Cyanidioschyzon merolae</i> for analysis of the target of rapamycin (TOR) function2017

    • Author(s)
      Imamura Sousuke、Taki Keiko、Tanaka Kan
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 63 Issue: 5 Pages: 305-309

    • DOI

      10.2323/jgam.2017.02.002

    • NAID

      130006206605

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A MYB-type transcription factor, MYB2, represses light-harvesting protein genes in Cyanidioschyzon merolae2017

    • Author(s)
      Kawase Yasuko、Imamura Sousuke、Tanaka Kan
    • Journal Title

      FEBS Letters

      Volume: 591 Issue: 16 Pages: 2439-2448

    • DOI

      10.1002/1873-3468.12763

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] E3ユビキチンリガーゼ Cul4 複合体の関わる単細胞紅藻光シグナル伝達経路2020

    • Author(s)
      北川美也子,小林勇気,吉川瞳子,大原ひかる,華岡光正,今村壮輔,田中寛
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンにおける窒素欠乏応答転写因子 MYB1 の活性化調節機構2020

    • Author(s)
      周柏峰,田中寛,今村壮輔
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンにおけるスターチ蓄積を調節する新規機構の解明2020

    • Author(s)
      Imran Pancha,島弘季,東谷なほ子,五十嵐和彦,東谷篤志,田中寛,今村壮輔
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Characterization of Target of Rapamycin (TOR) Complex in the Unicellular Red Alga2020

    • Author(s)
      Kaumeel Choksi, Kan Tanaka, Sousuke Imamura
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolaeにおいて ACRドメインリピートタンパク質 CmACR は窒素同化を 担うGS/GOGAT サイクルを統合する2020

    • Author(s)
      竹村時空,今村壮輔,田中寛
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大腸菌におけるピルビン酸応答転写因子 PdhR による ゲノム転写制御ネットワークの解明2020

    • Author(s)
      安西 拓実、今村 壮輔、石浜 明、島田 友裕
    • Organizer
      第14回日本ゲノム微生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Nitrogen-dependent regulation in the unicellular red alga Cyanidioschyzon merolae2019

    • Author(s)
      Sousuke Imamura
    • Organizer
      Institute of Plant Biochemistry Seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Underlying Regulatory Mechanism of Oil and Starch Accumulation in Microalgae to Enhance Its Biofuel Potential2019

    • Author(s)
      Sousuke Imamura
    • Organizer
      Fuel Science Center Seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Underlying molecular mechanisms of oil and starch accumulation in microalgae to enhance its biofuel potential2019

    • Author(s)
      Sousuke Imamura
    • Organizer
      ASEAN Bioenergy and Bioeconomy Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 小胞体局在型グリセロール3りん酸アシル基転移酵素の過剰発現による藻類オイル生産性の飛躍的向上2019

    • Author(s)
      福田智、平澤英里、竹村時空、高橋颯太、チョクシカウミール、パンチャイムラン、田中寛、今村壮輔
    • Organizer
      日本農芸化学会2019年度東京大会
    • Related Report
      2018 Research-status Report
  • [Presentation] おける葉緑体局在脂肪酸輸送体CmFAX1の同定とその応用2019

    • Author(s)
      竹村時空、今村壮輔、田中寛
    • Organizer
      日本農芸化学会2019年度東京大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ゲノム編集技術を用いた多重遺伝子破壊による単細胞緑藻(Coccomyxa)の油脂生産性改良2019

    • Author(s)
      井出曜子、早川准平、吉満勇也、糠塚明、小松さと子、今村壮輔、原山重明
    • Organizer
      日本農芸化学会2019年度東京大会
    • Related Report
      2018 Research-status Report
  • [Presentation] A forward genetics-based genome editing of a green alga Coccomyxa improved its oil productivity2019

    • Author(s)
      Yoko Ide, Jumpei Hayakawa, Yuya Yoshimitsu, Satoko Komatsu, Shuua Tagiri, Hiyori Fukahori, Sousuke Imamura, Shigeaki Harayama
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Characterization of chloroplast protein import in red alga Cyanidioschyzon merolae. Poster presentation2019

    • Author(s)
      Sanghun Baek, Yukari Asakura, Gaku Fujii, Sousuke Imamura, Kan Tanaka, Masato Nakai
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942における主要シグマ因子結合タンパク質の機能解析2019

    • Author(s)
      長谷川葉月、鶴巻達大、小林一幾、今村壮輔、田中寛
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおけるグルタミン結合ドメインリピートタンパク質CmACRの機能解析2019

    • Author(s)
      竹村時空、今村壮輔、田中寛
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] The checkpoint kinase TOR modulates chloroplast ribosomal RNA synthesis through expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) in a unicellular red alga2019

    • Author(s)
      Sousuke Imamura, Yuhta Nomura, Tokiaki Takemura, Imran Pancha, Keiko Taki, Kazuki Toguchi, Yuzuru Tozawa, Kan Tanaka
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] バクテリアにおける主要シグマ因子結合タンパク質と熱耐性2019

    • Author(s)
      長谷川葉月、鶴巻達大、小林一幾、今村壮輔、田中寛
    • Organizer
      第13回 日本ゲノム微生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Target of rapamycin (TOR) plays a critical role in carbon allocation in the unicellular red alga Cyanidioschyzon merolae2018

    • Author(s)
      mran Pancha, Hiroki Shima, Nahoko Higashitani, Kazuhiko Igarashi, Atsushi Higashitani, Kan Tanaka and Sousuke Imamura
    • Organizer
      EMBO Workshop: Target of rapamycin (TOR) signaling in photosynthetic organisms
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The checkpoint kinase TOR modulates chloroplast ribosomal RNA synthesis through expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) in a unicellular red alga2018

    • Author(s)
      Sousuke Imamura, Yuhta Nomura, Tokiaki Takemura, Imran Pancha, Keiko Taki, Kazuki Toguchi, Yuzuru Tozawa and Kan Tanaka
    • Organizer
      EMBO Workshop: Target of rapamycin (TOR) signaling in photosynthetic organisms
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Target of rapamycin (TOR) is a major signalling pathway that regulate starch accumulation in the unicellular red alga Cyanidioschyzon merolae2018

    • Author(s)
      Imran Pancha, Hiroki Shima, Nahoko Higashitani, Kazuhiko Igarashi, Atsushi Higashitani, Kan Tanaka,and Sousuke Imamura
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolaeにおけるACTドメインリピートタンパク質(ACR)の機能解析2018

    • Author(s)
      竹村 時空、今村 壮輔、小林 勇気、田中 寛
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおけるトリアシルグリセロール生合成に関わる遺伝子の発現制御メカニズムの解析2018

    • Author(s)
      高橋颯太、瀧景子、河瀬泰子、福田智、田中寛、今村壮輔
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 単細胞紅藻シゾンにおけるグリセロール-3リン酸アシルトランスフェラーゼ過剰発現によるトリアシルグリセロール蓄積機構の解析2018

    • Author(s)
      福田智、平澤英里、田中寛、今村壮輔
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Biochemical and genetic engineering strategies to improve the starch productivity in microalgae2017

    • Author(s)
      Imran Pancha, Kan Tanaka, and Sousuke Imamura
    • Organizer
      14th Young researchers seminar of Japanese society of photosynthesis research
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Construction of a rapamycin-susceptible strain of the unicellular red alga Cyanidioschyzon merolae for analysis of target of rapamycin (TOR) function2017

    • Author(s)
      Sousuke Imamura, Tomohiro Wakasugi, Keiko Taki, and Kan Tanaka
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 藻類における窒素欠乏応答機構の解明とその応用2017

    • Author(s)
      今村壮輔
    • Organizer
      第3回 植物の栄養研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Homogeneous Sn-Catalyzed Transformation of Algal Residue into Important Chemicals2017

    • Author(s)
      Sho Yamaguchi, Yuuki Kawada, Hidetaka Yuge, Kan Tanaka, Sousuke Imamura
    • Organizer
      16th Korea-Japan Symposium on Catalysis & 3rd International Symposium of Institute for Catalysis
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Book] Cyanidioschyzon merolae A New Model Eukaryote for Cell and Organelle Biology2017

    • Author(s)
      Imamura Sousuke、Tanaka Kan
    • Total Pages
      14
    • Publisher
      Springer
    • Related Report
      2017 Research-status Report
  • [Remarks] オイル生産性が飛躍的に向上したスーパー藻類を作出:バイオ燃料生産における最大の壁を打破

    • URL

      https://www.titech.ac.jp/news/2018/042168.html

    • Related Report
      2018 Research-status Report
  • [Remarks] 藻類のデンプン産出を自在にコントロール:環境に優しいプラスチックや医薬品の増産に期待

    • URL

      https://www.titech.ac.jp/news/2018/042844.html

    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] トリアシルグリセロール生産性が向上した微細藻類遺伝子組換え株及びトリアシルグリセロールの生産方法2018

    • Inventor(s)
      今村 壮輔、田中 寛、竹村 時空
    • Industrial Property Rights Holder
      東京工業大学、株式会社ユーグレナ
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-020354
    • Filing Date
      2018
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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