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Spatial and temporal studies of pollen-specific flavonoid metabolism and its working mechanism

Research Project

Project/Area Number 17K07460
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Yonekura-Sakakibara Keiko  国立研究開発法人理化学研究所, 環境資源科学研究センター, 上級研究員 (20360555)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords二次代謝 / フラボノイド / 配糖化酵素 / 稔性 / 花粉 / 植物
Outline of Final Research Achievements

Flavonoids are one of the major plant secondary metabolites and known to be involved in pollen fertility in petunia and maize. We have identified UGT79B31, a UDP-glycosyltransferase responsible for the terminal modification of petunia pollen-specific flavonoids. UGT79B31 encodes a flavonoid 3-O-glycoside: 2″-O-glucosyltransferase. UGT79B31 protein was localized in the cytosol. The flavonoid profiles in various organs petunia were revealed.

Academic Significance and Societal Importance of the Research Achievements

花粉特異的フラボノイドの代謝系酵素遺伝子の同定は、種子形成能に及ぼすフラボノイドの作用メカニズムの解明の手掛かりとなり、将来的には園芸学的な応用展開が期待される。
現在、約9000種類のフラボノイドが報告されているが、これまで修飾様式を含めたフラボノイドの詳細構造とその機能の関連性が明らかにされているものはほとんどなく、その多様性の意義は不明であった。陸上植物に普遍的に存在するフラボノイドの詳細な構造とその代謝経路について、基礎的知見を積み上げることは、生態学的、形態学的に多様な植物の二次代謝産物生合成能力の獲得メカニズムの理解につながる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] The Origin and Evolution of Plant Flavonoid Metabolism2019

    • Author(s)
      Yonekura-Sakakibara Keiko、Higashi Yasuhiro、Nakabayashi Ryo
    • Journal Title

      Frontiers in Plant Science

      Volume: 10 Pages: 3389-3389

    • DOI

      10.3389/fpls.2019.00943

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] UGT79B31 is responsible for the final modification step of pollen-specific flavonoid biosynthesis in Petunia hybrida2018

    • Author(s)
      Eva Knoch, Satoko Sugawara, Tetsuya Mori, Ryo Nakabayashi, Kazuki Saito, Keiko Yonekura-Sakakibara
    • Journal Title

      Planta

      Volume: 247 Issue: 4 Pages: 779-790

    • DOI

      10.1007/s00425-017-2822-5

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] UGT79B31 is responsible for the terminal modification step of pollen-specific flavonoid biosynthesis in Petunia hybrida2018

    • Author(s)
      Keiko Yonekura-Sakakibara
    • Organizer
      IPMB (International Plant Molecular Biology) 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Flavonoid Biosynthesis in Pollen: Functional Identification of Glycosyltransferases Responsible for Pollen-specific Flavonols2017

    • Author(s)
      Keiko Yonekura-Sakakibara
    • Organizer
      XIX International Botanical Congress
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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