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Analysis of the mechanism of regulation of the disappearance of excess lipid body in plant cells.

Research Project

Project/Area Number 17K07467
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionNiigata University

Principal Investigator

HAYASHI YASUKO  新潟大学, 自然科学系, 准教授 (20228597)

Co-Investigator(Kenkyū-buntansha) 及川 和聡  京都大学, 工学研究科, 特定講師 (70508457)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsリピッドボディ / シロイヌナズナ / マイクロボディ / ER / 子葉 / クラミドモナス / オートファジー / 貯蔵脂肪 / 子葉細胞 / chlamydomonas / 窒素飢餓 / オイルボディ / 電子顕微鏡 / ペルオキシソーム / グリオキシソーム / 免疫電子顕微鏡 / 緑藻 / 小胞体 / 細胞 / 植物
Outline of Final Research Achievements

In the cotyledonary cells of Arabidopsis, peroxisomes come into contact with lipid bodies and produce the necessary energy after germination. However, the presence of sucrose in the medium reduces this contact and does not reduce storage lipids. At this time, it was suggested that the endoplasmic reticulum may regulate the metabolic rate of peroxisomes by surrounding the lipid body. It was also shown that the excess storage lipids that became unnecessary due to the greening of cotyledons is reduced not only by the metabolism of peroxisomes but also by other disappearance mechanisms. In addition, it was shown that the lipid bodies accumulated in unicellular green algae cells of Chlamydomonas due to nitrogen deficiency may be degraded by a mechanism such as autophagy rather than the peroxisome metabolism system.

Academic Significance and Societal Importance of the Research Achievements

発芽後生育場所を変更できない植物にとっては、発芽後、光合成能を獲得するまでの生き残り戦略を有することは重要である。発芽しても光が得られず緑化が進行しない場合は、エネルギー源の消費速度を抑え、生存期間をできるだけ引き伸ばすことが必要であり、順調に光合成能が獲得された場合は、貯蔵脂肪を不用物として速やかに分解する機構の存在が必要である。本研究は、子葉細胞内において、これまでに知られていないリピッドボディの消失調節機構が存在する可能性を示した。本研究の結果は、将来的に地球の環境が大きく変化しても生き残ることができる植物の作出などにヒントを提供する可能性がある。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (12 results)

All 2022 2021 2019 2018 2017 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Bina Nusantara University/Institut Teknologi Bandung(インドネシア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Jagiellonian University(ポーランド)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Indonesian Institute of Sciences(インドネシア)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Ubiquitin-Conjugating Activity by PEX4 is Required for Efficient Protein Transport to Peroxisomes in Arabidopsis thaliana2022

    • Author(s)
      Mano Shoji、Hayashi Yasuko、Hikino Kazumi、Otomo Masayoshi、Kanai Masatake、Nishimura Mikio
    • Journal Title

      Journal of Biological Chemistry

      Volume: - Issue: 6 Pages: 102038-102038

    • DOI

      10.1016/j.jbc.2022.102038

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae2021

    • Author(s)
      Al-Azab Ayat J.S.、Widyaningrum Dwiyantari、Hirakawa Haruna、Hayashi Yashuko、Tanaka Satoshi、Ohama Takeshi
    • Journal Title

      Algal Research

      Volume: 54 Pages: 102191-102191

    • DOI

      10.1016/j.algal.2021.102191

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Re‐evaluation of physical interaction between plant peroxisomes and other organelles using live‐cell imaging techniques2019

    • Author(s)
      Oikawa Kazusato、Hayashi Makoto、Hayashi Yasuko、Nishimura Mikio
    • Journal Title

      Journal of Integrative Plant Biology

      Volume: 61 Issue: 7 Pages: 836-852

    • DOI

      10.1111/jipb.12805

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles2018

    • Author(s)
      Widyaningrum Dwiyantari、Iida Daisuke、Tanabe Yuma、Hayashi Yasuko、Kurniasih Sari Dewi、Ohama Takeshi
    • Journal Title

      Journal of Phycology

      Volume: 55 Issue: 1 Pages: 118-133

    • DOI

      10.1111/jpy.12798

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Existence of Lipid Bodies Surrounded by Membranes in Early Greening Cotyledonary Cells2018

    • Author(s)
      Yasuko Hayashi, Chinatsu Takagi and Mikio Nishimura
    • Journal Title

      Cytologia

      Volume: 83 Pages: 1-1

    • NAID

      130007394026

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Dwiyantari Widyaningrum, Ayat Alazab, Yasuko Hayashi , Akito Yamazaki and Takeshi Ohama2019

    • Author(s)
      プラスチック樹脂製ナノ粒子が広範囲の藻に誘導する細胞死メカニズムの解析
    • Organizer
      第 13 回クラミドモナス研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Acutely induced burst ROS generation and cell death on board algal species by resin nanoparticle exposure2019

    • Author(s)
      Ayat Alazab, Dwiyantari Widyaningrum, Yasuko Hayashi and Takeshi Ohama
    • Organizer
      5th International volvox conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] アクリル微粒子の緑藻に及ぼす影響2018

    • Author(s)
      高橋慶介,中野智美,田辺友眞,Dwiyantari Widyaningrum,大濱 武,林八寿子
    • Organizer
      日本植物形態学会
    • Related Report
      2018 Research-status Report
  • [Presentation] シロイヌナズナATG2変異体におけるPI3PとATG18aの局在解析2017

    • Author(s)
      霜田圭祐,早乙女真穂,及川和聡,真野昌二,西村幹夫,林八寿子
    • Organizer
      日本植物形態学会第29回大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2023-01-30  

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