Molecular basis of the intracellular symbiosis between deep-sea bivalve and the chemoautotrophic bacteria

• Project/Area Number: 17K07519
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: YOSHIDA Takao 国立研究開発法人海洋研究開発機構, 地球環境部門(海洋生物環境影響研究センター), 主任技術研究員 (60399566)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 共生 / 遺伝子発現 / 相互作用 / 進化 / 生体分子 / 遺伝子

Outline of Final Research Achievements

To elucidate the molecular mechanism for maintaining the endosymbiosis between host animal and chemosynthetic bacteria, dual transcriptome analysis of host and endosymbiont was conducted using bivalves living in chemosynthetic ecosystems. Comparing the methabolic pathways both host and symbionts, we found that host bivalve was shown to share the metabolic pathway including organic compound biosynthesis between host and endosymbiont, suggesting tight interaction in this endosymbiosis.

Academic Significance and Societal Importance of the Research Achievements

化学合成生態系に生息する多くの動物は化学合成独立栄養細菌との共生関係を持つ共生系が存在する。他の共生系とは大きく異なり、宿主の多くは口や消化管が退化的で自ら食物摂取せず、細胞内に共生する化学合成独立栄養細菌が無機炭素やメタンから合成した有機物に完全に依存するとされている。本研究により、このユニークな共生系の実態を明らかにすることができ、共生による生物適応生存戦略の理解を深めることができると期待される。

Research Products

• [Journal Article] Identification of cells expressing two peptidoglycan recognition proteins in the gill of the vent mussel, Bathymodiolus septemdierum (2019)
  • Author(s): Ikuta Tetsuro、Tame Akihiro、Saito Masaki、Aoki Yui、Nagai Yukiko、Sugimura Makoto、Inoue Koji、Fujikura Katsunori、Ohishi Kazue、Maruyama Tadashi、Yoshida Takao
  • Journal Title: Fish & Shellfish Immunology Volume: 93 Pages: 815-822
  • DOI: 10.1016/j.fsi.2019.08.022
  • Peer Reviewed / Open Access
• [Journal Article] Monoclonal antibodies against leukocytes in the beluga whale, <i>Delphinapterus leucas</i> (2018)
  • Author(s): Saito Chiaki、Ohishi Kazue、Kakizoe Yuka、Saito Masafumi、Mishima Hideki、Hongo Yuki、Nakamura Yoshimitsu、Nakazawa Masatoshi、Yoshida Takao、Maruyama Tadashi
  • Journal Title: JAMSTEC Report of Research and Development Volume: 27 Issue: 0 Pages: 119-126
  • DOI: 10.5918/jamstecr.27.119
  • NAID: 130007506155
  • ISSN: 1880-1153, 2186-358X
  • Year and Date: 2018-09-01
  • Peer Reviewed / Open Access
• [Journal Article] Morphological and functional characterization of hemocytes from two deep-sea vesicomyid clams Phreagena okutanii and Abyssogena phaseoliformis (2018)
  • Author(s): Tame Akihiro、Ozawa Genki、Maruyama Tadashi、Yoshida Takao
  • Journal Title: Fish & Shellfish Immunology Volume: 74 Pages: 281-294
  • DOI: 10.1016/j.fsi.2017.12.058
  • Peer Reviewed
• [Journal Article] Monoclonal antibodies that recognize symbiotic bacteria and hemocytes in the deep-sea vesicomyid clam <i>Phreagena okutanii</i> (2018)
  • Author(s): Ohishi Kazue、Nakamura Yoshimitsu、Kusaka Chiho、Nagai Yukiko、Nakazawa Masatoshi、Yoshida Takao、Maruyama Tadashi
  • Journal Title: JAMSTEC Report of Research and Development Volume: 26 Issue: 0 Pages: 75-83
  • DOI: 10.5918/jamstecr.26.75
  • NAID: 130006725614
  • ISSN: 1880-1153, 2186-358X
  • Peer Reviewed / Open Access
• [Book] 深海――極限の世界 生命と地球の謎に迫る （第２章１・２と１・３担当） (2019)
  • Author(s): 吉田 尊雄（藤倉 克則、木村 純一 編著 ）
  • Total Pages: 296
  • Publisher: 講談社
  • ISBN: 9784065160428