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Investigation of IRE1 activation mechanism for seed development and development of seed biomass modification technology

Research Project

Project/Area Number 17K07610
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionOsaka Prefecture University

Principal Investigator

Mishiba Kei-ichiro  大阪府立大学, 生命環境科学研究科, 准教授 (70390888)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords小胞体ストレス応答 / シロイヌナズナ / 種子 / IRE1 / 遺伝子導入 / ゲノム編集
Outline of Final Research Achievements

This study focused on the Arabidopsis IRE1C gene, whose product lacks a sensor domain, to investigate the role of sensor domain-independent IRE1 activation. The ire1a/b/c triple mutants were lethal, and heterozygous IRE1C mutation in the ire1a/b mutants resulted in growth defects and reduction of the number of pollen grains. Expression of a mutant form of IRE1B that lacks the luminal sensor domain (ΔLD) complemented the developmental defects. The ΔLD protein was activated by saturation of lipids, resulting in RIDD. These results suggest that the sensor domain-independent IRE1 activation may be involved in plant development.

Academic Significance and Societal Importance of the Research Achievements

分泌タンパク質が大量に作られる動物組織ではIRE1が働くことが報告されているが、この時に異常タンパク質が生じることでIRE1が働くものと考えられていた。本研究でセンサー領域を持たない(異常タンパク質を感知出来ない)IRE1が植物の発達過程で働くことが明らかになったことから、発達の過程で異常タンパク質が作られなくてもIRE1が働く仕組みが推定された。これまでIRE1は異常タンパク質のセンサーであることで注目されてきたが、今回の研究では異常タンパク質を必要としないIRE1の働きが、植物の発達に貢献していることを示した。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 Other

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Deficiency in the double-stranded RNA binding protein HYPONASTIC LEAVES1 increases sensitivity to the endoplasmic reticulum stress inducer tunicamycin in Arabidopsis.2019

    • Author(s)
      Rikako Hirata, Kei-ichiro Mishiba, Nozomu Koizumi, Yuji Iwata
    • Journal Title

      BMC Research Notes

      Volume: 12 Issue: 1 Pages: 580-580

    • DOI

      10.1186/s13104-019-4623-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unfolded protein-independent IRE1 activation contributes to multifaceted developmental processes in Arabidopsis2019

    • Author(s)
      Mishiba Kei-ichiro、Iwata Yuji、Mochizuki Tomofumi、Matsumura Atsushi、Nishioka Nanami、Hirata Rikako、Koizumi Nozomu
    • Journal Title

      Life Science Alliance

      Volume: 2 Issue: 5 Pages: e201900459-e201900459

    • DOI

      10.26508/lsa.201900459

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Constitutive BiP protein accumulation in Arabidopsis mutants defective in a gene encoding chloroplast-resident stearoyl-acyl carrier protein desaturase2018

    • Author(s)
      Iwata Yuji、Iida Tsukasa、Matsunami Toshihiro、Yamada Yu、Mishiba Kei-ichiro、Ogawa Takumi、Kurata Tetsuya、Koizumi Nozomu
    • Journal Title

      Genes to Cells

      Volume: 23 Issue: 6 Pages: 456-465

    • DOI

      10.1111/gtc.12585

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inositol-requiring enzyme 1 affects meristematic division in roots under moderate salt stress in Arabidopsis2017

    • Author(s)
      Iwata Yuji、Yagi Fumika、Saito Sae、Mishiba Kei-ichiro、Koizumi Nozomu
    • Journal Title

      Plant Biotechnology

      Volume: 34 Issue: 3 Pages: 159-163

    • DOI

      10.5511/plantbiotechnology.17.0615a

    • NAID

      130006124648

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activation of the Arabidopsis membrane-bound transcription factor bZIP28 is mediated by site-2 protease, but not site-1 protease2017

    • Author(s)
      Yuji Iwata, Makoto Ashida, Chisa Hasegawa, Kazuki Tabara, Kei-ichiro Mishiba, Nozomu Koizumi
    • Journal Title

      The Plant Journal

      Volume: 印刷中 Issue: 3 Pages: 408-415

    • DOI

      10.1111/tpj.13572

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] センサードメイン非依存的なIRE1活性化がシロイヌナズナの発達に与える影響2019

    • Author(s)
      三柴啓一郎、岩田雄二、望月知史、松村篤、西岡七海、平田梨佳子、小泉望
    • Organizer
      第37回日本植物細胞分子生物学会(京都)大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナ膜結合型転写因子bZIP28の切断様式2017

    • Author(s)
      長谷川千紗、芦田誠、三柴啓一郎、岩田雄二、小泉望
    • Organizer
      日本農芸化学会(中四国・西日本支部)
    • Related Report
      2017 Research-status Report
  • [Presentation] 形質転換シロイヌナズナによる細胞レベルでの細胞質スプライシングの検出2017

    • Author(s)
      田原一喜、三柴啓一郎、小泉望、岩田雄二
    • Organizer
      日本農芸化学会(中四国・西日本支部)
    • Related Report
      2017 Research-status Report
  • [Presentation] ゼニゴケ小胞体ストレスセンサーIRE1の機能解析2017

    • Author(s)
      竹田翔、十川太輔、西浜竜一、三柴啓一郎、大和勝幸、河内孝之、岩田雄二、小泉望
    • Organizer
      日本農芸化学会(中四国・西日本支部)
    • Related Report
      2017 Research-status Report
  • [Remarks] 研究グループHP

    • URL

      http://www.biosci.osakafu-u.ac.jp/pmb/

    • Related Report
      2019 Annual Research Report 2018 Research-status Report 2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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