Project/Area Number |
17K07660
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Horticultural science
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
Nunome Tsukasa 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, ユニット長 (50355624)
|
Co-Investigator(Kenkyū-buntansha) |
山口 博隆 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 上級研究員 (30355664)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 単為結果性 / 果実形態形成 / トマト / ゲノム編集 / 単為結果 / 園芸学 / 育種学 / 遺伝学 |
Outline of Final Research Achievements |
To elucidate the mechanism of fruit formation and parthenocarpy by the PAT-2 gene and to improve the seed formation of parthenocarpic tomato fruit, mutants of the PAT-2 gene and the PAT-2 homolog genes, which works in cooperate with the PAT-2 gene, were generated by genome editing. The expression of PAT-2 was high in the early stage of flower bud formation and low in the late stage of flower bud formation, flowering and fruit growth. We selected the PAT-2 homologous genes with a similar expression pattern of PAT-2 gene and as a candidate for mutant production by genome editing. The PAT-2 mutant showed parthenocarpy, and one of the PAT-2 homologous gene mutant showed growth abnormalities in delayed and flower bud formation were observed, suggesting that they are involved in flower and fruit formation. Further analysis of the interaction between the PAT-2 and PAT-2 homologous genes, and the biosynthesis and metabolism of plant hormones is expected.
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Academic Significance and Societal Importance of the Research Achievements |
単為結果性は、訪花昆虫や着果剤の利用削減と着果安定が見込まれる形質であるが、単為結果性トマトは採種不安定性の改善が望まれている。トマト単為結果性PAT-2遺伝子は、ジンクフィンガー(ZF)とホメオドメイン(HD) をもつ植物に特有の転写因子(ZFHD)であることが報告されているが、その詳細な機能解析は行われていない。トマトでは23個のZFHDが推測され、そのなかでPAT-2遺伝子とPAT-2ホモログ遺伝子のひとつが花芽・果実形成に寄与することを明らかにした。これらの研究成果は、単為結果性トマトの採種性の改善、花芽・果実形成機構の解明に繋がると期待される。
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