Studies on saprophytic, parasitic and symbiotic strategies depending on secondary metabolite production in necrotrophic plant pathogens

• Project/Area Number: 17K07666
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Tottori University
• Principal Investigator: Kodama Motoichiro 鳥取大学, (連合)農学研究科(研究院), 教授 (00183343)
• Co-Investigator(Kenkyū-buntansha): 石原 亨 鳥取大学, 農学部, 教授 (80281103)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 植物病原菌 / 腐生菌 / 共生菌 / 二次代謝産物 / ゲノミクス / 遺伝子クラスター / ネクロトロフ

Outline of Final Research Achievements

As a result of coevolution with plants, phytopathogenic fungi have developed the ability to produce a wide variety of secondary metabolites (SM). These compounds contribute to the differentiation of pathogenicity as effector molecules in pathogenic fungi, and as survival and pathogenicity strategies in fungi, as well as in saprophytic (non-pathogenic), symbiotic and pathogenic fungi.
Through comparative and phylogenomic analyses using various filamentous fungi with different lifestyles, it has been shown that clusters of SM biosynthetic genes play an important role in the survival and pathogenicity strategies of necrotrophic pathogens, saprophytic fungi, and symbiotic fungi, as well as in the process of diversity formation and evolution. In addition, we revealed the possibility that horizontal transfer of gene clusters and pathogenic chromosomes may be involved.

Academic Significance and Societal Importance of the Research Achievements

A. alternata菌群は、腐生菌から共生菌、さらに高度に分化した寄生菌まで幅広く包含するユニークなグループであり、菌の腐生性および寄生性の全体像を理解するための特色ある対象生物である。この特性を最大限に活用し、糸状菌の特質でもある多彩なSM生合成系をターゲットにして、菌の生存・発病戦略の分子機構、多様性および進化を包括的に解析することが可能となった。腐生生活においても重要である二次代謝系の分子機構が解明されれば、寄生菌コントロールの一環として、腐生生活環をターゲットにするという全く新規な応用研究分野が創出され、そのインパクトは大である。

Research Products

• [Int'l Joint Research] Cornell University(米国)
• [Int'l Joint Research] AgResearch(ニュージーランド)
• [Int'l Joint Research] コーネル大学(米国)
• [Int'l Joint Research] AgResearch(ニュージーランド)
• [Int'l Joint Research] AgResearch(ニュージーランド)
• [Int'l Joint Research] Cornell University(米国)
• [Journal Article] Identification of methoxylchalcones produced in response to CuCl2 treatment and pathogen infection in barley (2021)
  • Author(s): Naoki Ube, Yuhka Katsuyama, Keisuke Kariya, Shin-ichi Tebayashi, Masayuki Sue, Takuji Tohnooka, Kotomi Ueno, Shin Taketa and Atsushi Ishihara
  • Journal Title: Phytochemistry Volume: 184 Pages: 112650-112650
  • DOI: 10.1016/j.phytochem.2020.112650
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Natural variation in the expression and catalytic activity of a naringenin 7-O-methyltransferase influences antifungal defenses in diverse rice cultivars (2020)
  • Author(s): Koichi Murata, Takashige Kitano, Riko Yoshimoto, Ryo Takata, Naoki Ube, Kotomi Ueno, Makoto Ueno, Yukinori Yabuta, Masayoshi Teraishi, Cynthia K. Holland, Georg Jander, Yutaka Okumoto, Naoki Mori, Atsushi Ishihara
  • Journal Title: The Plant Journal Volume: 101 Issue: 5 Pages: 1103-1117
  • DOI: 10.1111/tpj.14577
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mutations found in the Asc1 gene that confer susceptibility to the AAL-toxin in ancestral tomatoes from Peru and Mexico (2020)
  • Author(s): Tsuzuki, R., Cabrera Pintado, R.M., Kodama, M., Komatsu, K. and Arie, T. et al.
  • Journal Title: Plants Volume: 10 Issue: 1 Pages: 47-47
  • DOI: 10.3390/plants10010047
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Natural variation of diterpenoid phytoalexins in cultivated and wild rice species (2020)
  • Author(s): Keisuke Kariya, Naoki Ube, Makoto Ueno, Masayoshi Teraishi, Yutaka Okumoto, Naoki Mori, Kotomi Ueno and Atsushi Ishihara
  • Journal Title: Phytochemistry Volume: 180 Pages: 112518-112518
  • DOI: 10.1016/j.phytochem.2020.112518
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evolution of pathogenicity in the <i>Alternaria</i> plant pathogens (2019)
  • Author(s): 児玉基一朗
  • Journal Title: Japanese Journal of Phytopathology Volume: 85 Issue: 3 Pages: 175-178
  • DOI: 10.3186/jjphytopath.85.175
  • NAID: 130007729758
  • ISSN: 0031-9473, 1882-0484
  • Year and Date: 2019-08-25
  • Peer Reviewed
• [Journal Article] Isolation of isolactarane sesquiterpenes from a <i>Phlebia tremellosa</i> culture filtrate and their growth promotion effects on lettuce roots (2019)
  • Author(s): Ishihara Atsushi、Ashida Chisaki、Ube Naoki、Abe Masato、Hiyoshi Hidetaka、Umezu Kazuto、Endo Naoki、Sotome Kozue、Maekawa Nitaro、Nakagiri Akira、Osaki-Oka Kumiko、Ichiyanagi Tsuyoshi、Ueno Kotomi
  • Journal Title: Journal of Pesticide Science Volume: 44 Issue: 1 Pages: 9-14
  • DOI: 10.1584/jpestics.D18-056
  • NAID: 130007602769
  • ISSN: 1348-589X, 1349-0923
  • Year and Date: 2019-02-20
  • Peer Reviewed
• [Journal Article] Evolution of pathogenicity of Alternaria pathogens (2019)
  • Author(s): Kodama, M.
  • Journal Title: Journal of General Plant Pathology Volume: 85 Issue: 6 Pages: 471-474
  • DOI: 10.1007/s10327-019-00877-3
  • Peer Reviewed
• [Journal Article] Accumulation of 9- and 13-KODEs in response to jasmonic acid treatment and pathogenic infection in rice (2018)
  • Author(s): Nishiguchi Sayaka、Murata Koichi、Ube Naoki、Ueno Kotomi、Tebayashi Shin-ichi、Teraishi Masayoshi、Okumoto Yutaka、Mori Naoki、Ishihara Atsushi
  • Journal Title: Journal of Pesticide Science Volume: 43 Issue: 3 Pages: 191-197
  • DOI: 10.1584/jpestics.D18-022
  • NAID: 130007437390
  • ISSN: 1348-589X, 1349-0923
  • Year and Date: 2018-08-20
  • Peer Reviewed
• [Journal Article] Identification of antifungal compounds in the spent mushroom substrate of <i>Lentinula edodes</i> (2018)
  • Author(s): Ishihara Atsushi、Goto Noriko、Kikkawa Misa、Ube Naoki、Ushijima Shuji、Ueno Makoto、Ueno Kotomi、Osaki-Oka Kumiko
  • Journal Title: Journal of Pesticide Science Volume: 43 Issue: 2 Pages: 108-113
  • DOI: 10.1584/jpestics.D17-094
  • NAID: 130006743028
  • ISSN: 1348-589X, 1349-0923
  • Year and Date: 2018-05-17
  • Peer Reviewed
• [Journal Article] Molecular characterization of a novel mycovirus in Alternaria alternata manifesting two-sided effects: down-regulation of host growth and up-regulation of host plant pathogenicity. (2018)
  • Author(s): Okada R, Ichinose S, Takeshita K, Urayama S, Fukuhara T, Komatsu K, Arie T, Ishihara A, Egusa M, Kodama M, Moriyama H（責任著者）
  • Journal Title: Virology Volume: 458 Pages: 265-273
  • DOI: 10.1016/j.virol.2018.03.027
  • Peer Reviewed / Open Access
• [Journal Article] Total Biosynthesis of Antiangiogenic Agent (-)-Terpestacin by Artificial Reconstitution of the Biosynthetic Machinery in Aspergillus oryzae (2018)
  • Author(s): Narita Koji、Minami Atsushi、Ozaki Taro、Liu Chengwei、Kodama Motoichiro、Oikawa Hideaki
  • Journal Title: The Journal of Organic Chemistry Volume: 83 Issue: 13 Pages: 7042-7048
  • DOI: 10.1021/acs.joc.7b03220
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clues to an Evolutionary Mystery: The Genes for T-Toxin, Enabler of the Devastating 1970 Southern Corn Leaf Blight Epidemic, Are Present in Ancestral Species, Suggesting an Ancient Origin (2018)
  • Author(s): Condon Bradford J.、Elliott Candace、Gonz?lez Jonathan B.、Yun Sung Hwan、Akagi Yasunori、Wiesner-Hanks Tyr、Kodama Motochiro、Turgeon B. Gillian
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 31 Issue: 11 Pages: 1154-1165
  • DOI: 10.1094/mpmi-03-18-0070-r
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Focused genome mining of structurally related sesterterpenes: enzymatic formation of enantiomeric and diastereomeric products (2017)
  • Author(s): Narita, K., Sato, H., Minami, A., Kudo, K., Gao, L., Liu, C., Ozaki, T., Kodama, M.., Lei, X., Taniguchi, T., Monde, K., Yamazaki, M., Uchiyama, M., Oikawa, H.
  • Journal Title: Organic letters Volume: 19 Issue: 24 Pages: 6696-6699
  • DOI: 10.1021/acs.orglett.7b03418
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 沖縄県内サトウキビ圃場から分離されたBacillus属菌のサトウキビ黒穂病菌およびバナナパナマ病菌に対する阻害活性 (2021)
  • Author(s): 福井瑛士・伊藤通浩・新里尚也・伊禮 信・有江 力・児玉基一朗
  • Organizer: 令和3年度日本植物病理学会大会
• [Presentation] AAL 毒素感受性を決定するAsc1 遺伝子の多様性解析に基づくトマト栽培化・進化に関する研究 (2021)
  • Author(s): 都筑 麟・Medali Heidi Huarhua Zaquinaula・Liliana Maria Aragon Caballero・児玉 基一朗・小松 健・有江 力
  • Organizer: 令和3年度日本植物病理学会大会
• [Presentation] 日本各地のカモジグサ類 (Elymus) から分離されたEpichloe属エンドファイトの遺伝的多様性および二次代謝産物生合成能 (2020)
  • Author(s): 伊津奈織, 辻本 壽, Wayne R. Simpson, Richard D. Johnson, 赤木靖典, 石原亨, 児玉基一朗
  • Organizer: 令和2年度日本植物病理学会大会
• [Presentation] 沖縄県内サトウキビ圃場から分離されたサトウキビ黒穂病菌胞子発芽阻害活性を示す細菌 (2020)
  • Author(s): 福井瑛士・伊藤通浩・新里尚也・伊禮 信・有江 力・児玉基一朗
  • Organizer: 令和2年度日本植物病理学会関西部会
• [Presentation] Alternaria属菌の病原性進化に関する研究 (2019)
  • Author(s): 児玉基一朗
  • Organizer: 平成31年度日本植物病理学会大会
  • Invited
• [Presentation] 鳥取県および北海道産カモジグサ類 (Elymus) と共生するEpichloe属エンドファイトの二次代謝産物生合成能 (2019)
  • Author(s): 伊津奈織, 辻本 壽, Wayne R. Simpson, Richard D. Johnson, 赤木靖典, 石原亨, 児玉基一朗
  • Organizer: 令和元年度日本植物病理学会関西部会
• [Presentation] Genetic diversity and secondary metabolite biosynthetic potential of Epichloeendophytes isolated from Elymus species in various parts of Japan (2019)
  • Author(s): Naori Izu, Hitoshi Tsujimoto, Wayne R. Simpson, Richard D. Johnson, Yasunori Akagi, Atsushi Ishihara, Motoichiro Kodama
  • Organizer: International Symposium on Agricultural, Food, Environmental, and Life Sciences in Asia, 2019 (AFELiSA 2019)
  • Int'l Joint Research
• [Presentation] Alternaria alternataトマト病原型が保有する病原性染色体の起源 (2019)
  • Author(s): 赤木靖典・柘植尚志・有江力・児玉基一朗
  • Organizer: 日本植物病理学会2019年度大会、つくば市
• [Presentation] 日本各地においてカモジグサ類 (Elymus) から分離されたEpichloe属エンドファイトの遺伝的多様性 (2019)
  • Author(s): 伊津奈織・辻本 壽・Wayne R. Simpson ・Richard D. Johnson ・赤木靖典・児玉基一朗
  • Organizer: 日本植物病理学会2019年度大会、つくば市
• [Presentation] Corynespora cassiicolaが保有するT-toxin生合成遺伝子クラスター (2019)
  • Author(s): 岡本 愛・福田晃平・赤木靖典・下元祥史・曵地康史・Gillian.B.Turgeon・児玉基一朗
  • Organizer: 日本植物病理学会2019年度大会、つくば市
• [Presentation] 鳥取県内のカモジグサ類 (Elymus) と共生するEpichloe属エンドファイトの遺伝的多様性 (2018)
  • Author(s): 伊津奈織・辻本 壽・Wayne R. Simpson・Richard D. Johnson・赤木靖典・児玉基一朗
  • Organizer: 平成30年度植物微生物研究会、鳥取市
• [Presentation] 鳥取県内のカモジグサ類（Elymus）と共生するEpichloe属エンドファイトの遺伝的多様性解析 (2018)
  • Author(s): 伊津奈織・辻本 壽・Wayne R. Simpson・Richard D. Johnson・赤木靖典・児玉基一朗
  • Organizer: 平成30年度日本植物病理学会関西部会、山口市
• [Presentation] “病原性染色体”が支配するAlternaria属植物病原菌の進化と多様性形成 (2018)
  • Author(s): 児玉基一朗
  • Organizer: 第44回岡山植物病理セミナー「多賀シンポジウム」、岡山市
  • Invited
• [Presentation] タバコ赤星病菌のテヌアゾン酸生合成遺伝子AtNPS5は宿主タバコに対する病原力に関与する (2017)
  • Author(s): 赤木靖典・新城 亮・三木淳一・柘植尚志・児玉基一朗
  • Organizer: 平成29年度日本植物病理学会大会
• [Presentation] トマトアルターナリア茎枯病菌におけるAAL毒素生合成遺伝子クラスターの発現調節に関わる転写因子ALT13はALT1のスプライシングに関与する (2017)
  • Author(s): 赤木靖典・柘植尚志・児玉基一朗
  • Organizer: 平成29年度日本植物病理学会関西部会