Toward the understanding of the post-transcriptional regulation in leaf nitrogen assimilation

• Project/Area Number: 17K07691
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Hokkaido University
• Principal Investigator: Takabayashi Atsushi 北海道大学, 低温科学研究所, 助教 (90546417)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 植物の窒素同化 / 植物の窒素代謝 / 窒素同化 / アミノ酸代謝 / 窒素代謝 / 植物 / 葉緑体 / 栄養学

Outline of Final Research Achievements

In this study, we aimed to elucidate the regulatory mechanism of the nitrogen assimilation pathway via ACR11 in plants. First, the free amino acid profile specific to ACR11-deficient mutants was relatively stable even under different growing conditions. Since this difference cannot be explained only by the fact that ACR11 can regulate the enzymatic activity of GS and GOGAT, we generated douvle or triple mutants of ASN2, AspAT3, and GDH1/2 with ACR11 and examined their amino acid profiles, but found no significant changes. In addition, no direct interaction between ACR11 and enzymes other than GS/GOGAT was observed. Currently, we are testing the possibility of its involvement in amino acid transport from chloroplasts.

Academic Significance and Societal Importance of the Research Achievements

本研究では、植物の窒素同化経路のACR11を介した制御について研究した。複数のアプローチを用いた検証を行ったものの、GS/GOGAT以外の酵素とACR11との直接の相互作用を示すことはできなかったた。そのため現在では、ACR11がGS/GOGAT以外の酵素を直接制御している可能性は低いと考えている。一方で、いくつかの実験結果から、ACR11はGS/GOGATサイクルを制御しているだけではなく、葉緑体から細胞質へのアミノ酸輸送に関与しているとの仮説にたどり着いた。現在はその検証を行っている。

Research Products

• [Journal Article] Formation of a PSI-PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens (2019)
  • Author(s): Furukawa, R., Aso M, Fujita, T., Akimoto, S., Tanaka, R., Tanaka, A., Yokono, M., and Takabayashi, A.
  • Journal Title: Journal of Plant Research Volume: 132 Issue: 6 Pages: 867-880
  • DOI: 10.1007/s10265-019-01138-2
  • NAID: 120006941693
  • Peer Reviewed
• [Journal Article] Horizontal Transfer of Promiscuous Activity from Nonphotosynthetic Bacteria Contributed to Evolution of Chlorophyll Degradation Pathway (2019)
  • Author(s): Obata Daichi、Takabayashi Atsushi、Tanaka Ryouichi、Tanaka Ayumi、Ito Hisashi
  • Journal Title: Molecular Biology and Evolution Volume: 36 Issue: 12 Pages: 2830-2841
  • DOI: 10.1093/molbev/msz193
  • Peer Reviewed / Open Access
• [Journal Article] The PSI-PSII megacomplex in green plants. (2019)
  • Author(s): Yokono M, Takabayashi A, Kishimoto J, Fujita T, Iwai M, Murakami A, Akimoto S, Tanaka A.
  • Journal Title: Plant Cell Physiol. Volume: 印刷中 Issue: 5 Pages: 1098-1108
  • DOI: 10.1093/pcp/pcz026
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Regulation of excitation energy in Nannochloropsis photosystem II (2019)
  • Author(s): Yokono M, Umetani I, Takabayashi A, Akimoto S, Tanaka A.
  • Journal Title: Photosynth. Res. Volume: 139 Issue: 1-3 Pages: 155-161
  • DOI: 10.1007/s11120-018-0510-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evidence of the supercomplex organization of photosystem II and light-harvesting complexes in Nannochloropsis granulata. (2018)
  • Author(s): Umetani I, Kunugi M, Yokono M, Takabayashi A, Tanaka A.
  • Journal Title: Photosynth. Res. Volume: 136 Issue: 1 Pages: 49-61
  • DOI: 10.1007/s11120-017-0438-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Deficiency of the Stroma-Lamellar Protein LIL8/PSB33 Affects Energy Transfer Around PSI in Arabidopsis (2017)
  • Author(s): Kato Yukako、Yokono Makio、Akimoto Seiji、Takabayashi Atsushi、Tanaka Ayumi、Tanaka Ryouichi
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 11 Pages: 2026-2039
  • DOI: 10.1093/pcp/pcx124
  • NAID: 120006533129
  • Peer Reviewed
• [Presentation] ブルーネイティブ電気泳動を用いた光合成生物タンパク質複合体の網羅的解析 (2017)
  • Author(s): 高林厚史
  • Organizer: 第68回日本電気泳動学会総会
  • Invited
• [Remarks] 生物適応研究室
  • URL: http://www.lowtem.hokudai.ac.jp/plantadapt/