| Project/Area Number |
17K07704
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Okazaki Keiki 国立研究開発法人農業・食品産業技術総合研究機構, 中央農業研究センター, 上級研究員 (40414750)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ダイズ / メタボロミクス / GC/MS / 土壌水分 / 乾燥 / 過湿 / 代謝産物 / RNA-seq / 簡易分析 / 湿害 / VOC / 生産性 / 無機元素 / 代謝産物解析 / 窒素動態 |
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| Outline of Final Research Achievements |
Based on pot experiments, physiological response to soil capacity, dryness, and flooding conditions was carried out by analysis of metabolites. As a result, it was indicated that a certain influence was recognized in the shoot and the root by each stressor. Four experiments were analyzed and inositols was selected as indices with a consistent response to drought stress. Regarding flooding stress, it was found that changes occur in the ground part, such as the effect remaining on the metabolite profile even after 2 weeks of treatment. RNA-seq investigated the effects of the history of flooding stress on gene expression in subsequent dry stress conditions, and clarified that certain effects remain.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、ダイズの乾燥および過湿条件等の組み合わせによる影響メカニズムの理解が進展し、ストレスの指標となる複数の成分を特定することに成功した。これらの知見は、今後気候変動環境下における作物の頑強性向上に資すると期待される。
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