The molecular mechanism underlying nitrogen homeostasis in plants

• Project/Area Number: 17K07705
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Nagoya University (2018-2019); Institute of Physical and Chemical Research (2017)
• Principal Investigator: Kiba Takatoshi 名古屋大学, 生命農学研究科, 准教授 (20532097)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 窒素栄養 / 恒常性 / 窒素欠乏 / 転写制御 / 窒素栄養恒常性 / NIGT1 / リン欠乏 / 欠乏応答 / 転写因子 / 窒素飢餓 / 窒素恒常性 / 窒素不足 / 植物栄養

Outline of Final Research Achievements

Nitrogen homeostasis is crucial for plants to survive under fluctuating nutrient environments. It is maintained through the coordination of nitrogen-starvation and nitrogen-sufficiency responses. In this study, we identified a group of transcription factors, named NIGT1s, as a balancing factor between nitrogen-starvation and nitrogen-sufficiency responses. In addition, we revealed that NIGT1s act as a hub for integrating nitrogen and phosphorus signals to optimize nitrogen-phosphorus nutritional balance.

Academic Significance and Societal Importance of the Research Achievements

本研究では、古くから研究されてきた植物の窒素栄養応答の仕組みについて新たな分子メカニズムを明らかにした。また植物が複数の栄養応答を統御するメカニズムも解明しており、学術的意義は大きいと考える。
近年窒素やリン肥料の過剰な使用が、コスト高を招いているだけでなく深刻な環境問題をも引き起こしており、世界的に低インプット型持続的農業への移行が求められている。本研究成果は、野生種植物の高効率栄養獲得と利用のメカニズムの一端を明らかにしたものであり、低インプット型持続的農業に適した優良栽培作物創出につながると期待される。

Research Products

• [Int'l Joint Research] INRA(フランス)
• [Int'l Joint Research] INRA(フランス)
• [Journal Article] Nitrate‐inducible NIGT1 proteins modulate phosphate uptake and starvation signalling via transcriptional regulation of SPX genes (2020)
  • Author(s): Ueda Yoshiaki、Kiba Takatoshi、Yanagisawa Shuichi
  • Journal Title: The Plant Journal Volume: 102 Issue: 3 Pages: 448-466
  • DOI: 10.1111/tpj.14637
  • Peer Reviewed / Open Access
• [Journal Article] Harnessing symbiotic plant-fungus interactions to unleash hidden forces from extreme plant ecosystems (2020)
  • Author(s): Perez-Alonso M.M., Guerrero-Galan, C., Scholz, S.S., Kiba, T., Sakakibara, H., Ludwig-Muller, J., Krapp, A., Oelmuller, R., Vicente-Carbajosa, J., Pollmann, S.
  • Journal Title: Journal of Experimental Botany Volume: 54 Issue: 13 Pages: 137-142
  • DOI: 10.1093/jxb/eraa040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO2 (2019)
  • Author(s): Kiba Takatoshi、Takebayashi Yumiko、Kojima Mikiko、Sakakibara Hitoshi
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 7765-7765
  • DOI: 10.1038/s41598-019-44185-4
  • Peer Reviewed / Open Access
• [Journal Article] Genome sequence of Striga asiatica provides insight into the evolution of plant parasitism. (2019)
  • Author(s): Yoshida S. et al.
  • Journal Title: Current Biology Volume: 29 Issue: 18 Pages: 3041-3052
  • DOI: 10.1016/j.cub.2019.07.086
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice (2019)
  • Author(s): Toda Erika、Koiso Narumi、Takebayashi Arika、Ichikawa Masako、Kiba Takatoshi、Osakabe Keishi、Osakabe Yuriko、Sakakibara Hitoshi、Kato Norio、Okamoto Takashi
  • Journal Title: Nature Plants Volume: 5 Issue: 4 Pages: 363-368
  • DOI: 10.1038/s41477-019-0386-z
  • Peer Reviewed / Open Access
• [Journal Article] Shoot nitrate underlies a perception of nitrogen satiety to trigger local and systemic signaling cascades in Arabidopsis thaliana. (2019)
  • Author(s): Okamoto Y, Suzuki T, Sugiura D, Kiba T, Sakakibara H, Hachiya T.
  • Journal Title: Soil science and plant nutrition Volume: 65 Issue: 1 Pages: 56-64
  • DOI: 10.1080/00380768.2018.1537643
  • NAID: 210000012050
  • Peer Reviewed / Open Access
• [Journal Article] Repression of nitrogen-starvation responses by members of the Arabidopsis GARP-type transcription factor NIGT1/HRS1 subfamily. (2018)
  • Author(s): Kiba, T., Inaba, J., Kudo, T., Ueda, N., Konishi, M., Mitsuda, N, Takiguchi, Y., Kondou, Y., Yoshizumi, T., Ohme-Takagi, M., Matsui, M., Yano, K., Yanagisawa, S., and Sakakibara, H.
  • Journal Title: Plant Cell Volume: 30 Issue: 4 Pages: 925-945
  • DOI: 10.1105/tpc.17.00810
  • Peer Reviewed / Open Access
• [Journal Article] International Symposium on Long-Distance Signaling in Plants (2018)
  • Author(s): Kiba, T.
  • Journal Title: Plant Cell Physiol. Volume: 59 Issue: 9 Pages: 1697-1699
  • DOI: 10.1093/pcp/pcy151
  • Peer Reviewed / Open Access
• [Journal Article] SUPERMAN regulates floral whorl boundaries through control of auxin biosynthesis. (2018)
  • Author(s): Xu, Y., Prunet, N., Gan, E-S., Wang, Y., Stewart, D., Wellmer, F., Huang, J., Yamaguchi, N., Tatsumi, Y., Kojima, M., Kiba, T., Sakakibara, H., Jack, T.P., Meyerowitz, E.M., Ito, T.
  • Journal Title: EMBO Journal Volume: - Issue: 11
  • DOI: 10.15252/embj.201797499
  • NAID: 120006478564
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy. (2018)
  • Author(s): Yamaguchi, N., Huang, J., Tatsumi, Y., Abe, M., Sugano, S. S., Kojima, M., Takebayashi, Y., Kiba, T., Yokoyama, R., Nishitani, K., Sakakibara, H., and Ito, T.
  • Journal Title: Nature Commun. Volume: 9 Issue: 1 Pages: 5290-5290
  • DOI: 10.1038/s41467-018-07763-0
  • NAID: 120006549567
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis (2018)
  • Author(s): Maeda Yoshie、Konishi Mineko、Kiba Takatoshi、Sakuraba Yasuhito、Sawaki Naoya、Kurai Tomohiro、Ueda Yoshiaki、Sakakibara Hitoshi、Yanagisawa Shuichi
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 1-14
  • DOI: 10.1038/s41467-018-03832-6
  • Peer Reviewed / Open Access
• [Journal Article] Systemic transport of trans-zeatin and its precursor have differing roles in Arabidopsis shoots (2017)
  • Author(s): Osugi Asami、Kojima Mikiko、Takebayashi Yumiko、Ueda Nanae、Kiba Takatoshi、Sakakibara Hitoshi
  • Journal Title: Nature Plants Volume: 3 Issue: 8 Pages: 17112-17112
  • DOI: 10.1038/nplants.2017.112
  • Peer Reviewed
• [Journal Article] Inter-species hormonal control of host root morphology by parasitic plants. (2017)
  • Author(s): Spallek, T., Melnyk, C.W., Wakatake, T., Zhang, J., Sakamoto, Y., Kiba, T., Yoshida, S., Matsunaga, S., Sakakibara, H., *Shirasu, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 印刷中 Issue: 20 Pages: 5283-5288
  • DOI: 10.1073/pnas.1619078114
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis (2017)
  • Author(s): Pudasaini Ashutosh、Shim Jae Sung、Song Young Hun、Shi Hua、Kiba Takatoshi、Somers David E、Imaizumi Takato、Zoltowski Brian D
  • Journal Title: eLife Volume: 6 Pages: 1-27
  • DOI: 10.7554/elife.21646
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Nitrogen starvation responses in plants (2019)
  • Author(s): Takatoshi Kiba
  • Organizer: The 3rd International Conference on Life Sciences and Biotechnology
  • Int'l Joint Research / Invited
• [Presentation] A mechanism for xylem loading of cytokinin mediated by ABCG14 (2019)
  • Author(s): Takatoshi Kiba, Hitoshi Sakakibara
  • Organizer: The International Plant Growth Substances Association Conference
  • Int'l Joint Research
• [Presentation] Metabolomic and Transcriptomic Analysis of Metabolic Pathways Regulated by Nitrogen Starvation Response Regulator AtNIGT1s (2019)
  • Author(s): Takatoshi Kiba, Hitoshi Sakakibara
  • Organizer: The 4th International Symposium on the Nitrogen Nutrition of Plants
  • Int'l Joint Research
• [Presentation] Screening for the novel cytokinin transporter (2019)
  • Author(s): 浦上拓也、木羽隆敏、榊原均
  • Organizer: Frontiers in plant environmental response research
  • Int'l Joint Research
• [Presentation] Mechanism of ABCG14-mediated root-to-shoot cytokinin transport (2019)
  • Author(s): 橋本祐典、木羽隆敏、榊原均
  • Organizer: Frontiers in plant environmental response research
  • Int'l Joint Research
• [Presentation] Regulation of nitrogen acquisition under low availability and beyond (2019)
  • Author(s): Takatoshi Kiba
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Nitrogen starvation responses in Arabidopsis: transcriptional and metabolic regulations (2019)
  • Author(s): Takatoshi Kiba
  • Organizer: Japan-Taiwan Plant Biology 2019
  • Int'l Joint Research
• [Presentation] シロイヌナズナにおいてNIGT1転写因子群は窒素条件依存的にリンシグナルを正に調節する (2019)
  • Author(s): 植田佳明，木羽隆敏，柳澤修一
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] イネ受精卵を用いたDNAおよび選抜マーカーフリーなゲノム編集技術の確立と他作物種への応用 (2019)
  • Author(s): 戸田絵梨香，古磯成美，Tety Maryenti，竹林有理佳，市川雅子，木羽隆敏，刑部敬史，刑部祐里子，榊原均，加藤紀夫，岡本龍史
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Mechanism of ABCG14-mediated root-to-shoot cytokinin transport (2019)
  • Author(s): 神谷麻友、稲葉ジュン、竹林裕美子、小嶋美紀子、木羽隆敏、榊原均
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 窒素飢餓応答制御とリン飢餓応答制御のクロストーク (2018)
  • Author(s): 木羽隆敏
  • Organizer: 日本植物学会第82回大会
• [Presentation] Regulation of nitrogen acquisition under low availability (2017)
  • Author(s): Takatoshi Kiba
  • Organizer: Bilateral Closure Symposium of GDRI Integrative Plant Biology Network Program
  • Int'l Joint Research / Invited
• [Presentation] Structural Variations of Long-Distance Translocated Cytokinins Control the Specificity of the Action in Shoot Growth and Development (2017)
  • Author(s): Takatoshi Kiba
  • Organizer: XIX International Botanical Congress
  • Int'l Joint Research
• [Remarks] 名古屋大学 大学院生命農学研究科 植物情報分子研究室
  • URL: https://www.agr.nagoya-u.ac.jp/~ck/
• [Remarks] 名古屋大学生命農学研究科植物情報分子研究室
  • URL: https://www.agr.nagoya-u.ac.jp/~ck/