| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
This study aims to clarify the relationship between autophagy and the known vesicular traffic by analyzing the vesicular-trafficking factors required for autophagy in yeast. A new mutant of Ykt6, one of the vesicular-fusion factors, was made and used to show genetically that Ykt6 is necessary for the fusion of autophagosomes and the vacuole. The mutants defective in the vesicular trafficking were extracted from the yeast deletion library and used to determine the autophagic activity. We found that phosphate starvation-induced autophagy was significantly decreased in the mutants defective in the endosome to the late Golgi, suggesting that this pathway is involved in phosphate starvation-induced autophagy.
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