Adaptive immunity of fish mucosal tissues against protozoan parasites

• Project/Area Number: 17K07939
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Kyushu University
• Principal Investigator: Somamoto Tomonori 九州大学, 農学研究院, 准教授 (40403993)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 寄生原虫 / T細胞 / 魚類 / 細胞傷害 / 免疫 / 鰓 / 粘膜免疫 / 白点虫 / 抗原認識受容体 / CD8陽性T細胞 / ギンブナ / NCCRP-1 / 寄生虫 / 細胞傷害活性 / 傷害機構 / 獲得免疫

Outline of Final Research Achievements

The present study has shown that CD8-positive T cells from gill of ginbuna crucian carp directly kill protozoan parasite, Ichthyophthirius multifiliis. CD8-positive T cells recognized the parasite by NCCRP-1, which is a receptor for innate immunity, indicating that teleost T cells possess a unique parasite recognition system. The findings obtained in this study suggest that CD8-positive T cell plays an important role in eliminating the parasite in the mucosal tissue of fish.

Academic Significance and Societal Importance of the Research Achievements

現在の水産養殖では、様々な寄生虫類の感染症が問題となっているものの、寄生虫は発育ステージによって抗原性が変化すること、寄生部位が限定されることから、宿主の防御メカニズムを解明することが困難とされている。特に魚類のT細胞の寄生虫に対する機能は殆んど分かっていなかった。本研究では、魚類のT細胞は、寄生原虫に対する防御機構において重要な細胞あり、自然免疫担当の受容体で寄生虫を認識するという哺乳類ではみられない特徴を有していることを明らかにした。この研究成果は水産養殖業における寄生虫感染症に対する予防法の開発への貢献や比較免疫学へ重要な知見を付与するものである。

Research Products

• [Journal Article] Innate cell-mediated cytotoxicity of CD8+ T cells against the protozoan parasite Ichthyophthirius multifiliis in the ginbuna crucian carp, Carassius auratus langsdorfii. (2021)
  • Author(s): Sukeda M, Shiota K, Kondo M, Nagasawa T, Nakao M, Somamoto T
  • Journal Title: Developmental and Comparative Immunology Volume: 115 Pages: 103886-103886
  • DOI: 10.1016/j.dci.2020.103886
  • NAID: 120007188795
  • Peer Reviewed
• [Journal Article] 魚類の粘膜を介したワクチン投与法 (2020)
  • Author(s): 杣本智軌
  • Journal Title: アクアネット （湊文社） Volume: 7 Pages: 54-57
• [Journal Article] Mucosal delivery of fish vaccines: Local and systemic immunity following mucosal immunisations. (2020)
  • Author(s): Somamoto T., Nakanishi T.
  • Journal Title: Fish & Shellfish Immunology Volume: 99 Pages: 199-207
  • DOI: 10.1016/j.fsi.2020.01.005
  • NAID: 120007001038
  • Peer Reviewed
• [Journal Article] Generation of virus-specific CD8+ T cells by vaccination with inactivated virus in the intestine of ginbuna crucian carp. (2019)
  • Author(s): Tajimi S, Kondo M, Nakanishi T, Nagasawa T, Nakao M, Somamoto T.
  • Journal Title: Developmental and Comparative Immunology Volume: 93 Pages: 37-44
  • DOI: 10.1016/j.dci.2018.12.009
  • NAID: 120006819494
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of anti-atypical Aeromonas salmonicida monoclonal antibodies for diagnosis of new ulcer disease in koi carp. (2018)
  • Author(s): Somamoto T, Maruyama S, Nagasawa T, Nakao M, Sato A, Hatta H, Sato M, Murakami-Yamaguchi Y, Kizu-Mori K, Hirakawa Y, Narita H.
  • Journal Title: Fish Pathology Volume: 53 Pages: 36-39
  • NAID: 130006619196
  • Peer Reviewed
• [Journal Article] Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish: Implication for the robust innate defense mechanisms of teleosts. (2017)
  • Author(s): Tokunaga Y, Shirouzu M, Sugahara R, Yoshiura Y, Kiryu I, Ototake M, Nagasawa T, Somamoto T, Nakao M.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 7536-7536
  • DOI: 10.1038/s41598-017-08000-2
  • Peer Reviewed / Open Access
• [Presentation] 魚類CD8+ T細胞のMHC非依存的な細胞媒介性細胞傷害における寄生原虫認識受容体の探索 (2021)
  • Author(s): 助田将樹,長澤貴宏,中尾実樹,杣本智軌
  • Organizer: 日本水産学会春季大会
• [Presentation] 魚類リポカリンタンパク質 TBT-bp2 破壊メダカにおける白点虫感染機構の解析 (2021)
  • Author(s): 高井優生, 助田将樹, 高村匠, 長野陽介, 島崎洋平, 杣本智軌, 大嶋雄治
  • Organizer: 日本水産学会春季大会
• [Presentation] ギンブナの細胞外寄生原虫に対する 細胞傷害機構におけるシグナル伝達経路の探索 (2020)
  • Author(s): 助田将樹・長澤貴宏・塩田昂明・中尾実樹・ 杣本智軌
  • Organizer: 日本水産学会春季大会
• [Presentation] Direct cytotoxic activity of CD8+ T cells against Ichthyophthirius multifiliis in ginbuna crucian carp, Carassius auratus langsdorfii (2019)
  • Author(s): Masaki Sukeda, Koumei Shiota, Takahiro Nagasawa, Miki Nakao, Tomonori Somamoto
  • Organizer: The international society of fish and shellfish immunology
  • Int'l Joint Research
• [Presentation] 繊毛虫Ichtyophthirius multifiliisの成長段階の違いによるギンブナの免疫応答の比較 (2019)
  • Author(s): 塩田昂明・助田将樹・中西照幸・長澤貴宏・中尾実樹・杣本智軌
  • Organizer: 日本水産学会春季大会
• [Presentation] ギンブナCD8+Tリンパ球の寄生原虫に対する細胞遊走活性 (2018)
  • Author(s): 助田将樹・塩田昴明・長沢貴宏・中尾実樹・杣本智軌
  • Organizer: 日本水産学会秋季大会
• [Presentation] Homeostatic functional analysis of Tecrem, a CD46-like complement regulatory protein, on epithelial cells in carp. (2018)
  • Author(s): Prakash H, Motobe S, Nagasawa T, Somamoto T, Nakao M.
  • Organizer: 日本水産学会春季大会
• [Presentation] ギンブナCD8+Tリンパ球の細胞外寄生原虫に対する細胞傷害機構の解明 (2018)
  • Author(s): 助田将樹・長沢貴宏・中尾実樹・杣本智軌
  • Organizer: 日本水産学会春季大会
• [Presentation] 繊毛虫Ichthyophthirius multifiliisに対する魚類CD8+T細胞の傷害機構 (2017)
  • Author(s): 助田将樹,長沢貴宏,中尾実樹,杣本智軌
  • Organizer: 日本魚病学会秋季大会
• [Presentation] Ligand recognition specificity of carp thrombocytes as the natural type I IFN producing cells. (2017)
  • Author(s): Nagasawa T, Somamoto T, Nakao M.
  • Organizer: 日本水産学会創立85周年記念国際シンポジウム 「Fisheries Science for Future Generations」
  • Int'l Joint Research
• [Presentation] Functional analysis of tecrem, a CD46-like complement regulatory protein, on epithelial cells in the common carp. (2017)
  • Author(s): Prakash H, Motobe S, Nagasawa T, Somamoto T , Nakao M.
  • Organizer: 日本水産学会創立85周年記念国際シンポジウム 「Fisheries Science for Future Generations」
  • Int'l Joint Research
• [Presentation] 卵黄抗体で鯉の穴あき病予防 (2017)
  • Author(s): 杣本智軌
  • Organizer: 日本たまご研究会
  • Invited