• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of high-quality novel ES / iPS cells that precisely reflect the in vivo state

Research Project

Project/Area Number 17K08135
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionShiga University of Medical Science

Principal Investigator

TSUKIYAMA Tomoyuki  滋賀医科大学, 動物生命科学研究センター, 特任准教授 (60612132)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsiPS細胞 / ES細胞 / カニクイザル / 霊長類 / ゲノム編集 / CRISPR/Cas9 / 多能性幹細胞
Outline of Final Research Achievements

We generated reporters for establishing high-quality novel human and cynomolgus monkey ES / iPS cells that precisely reflect the in vivo state, and performed reporter knock-in on cynomolgus monkey ES cells. In this process, we established a cynomolgus monkey ES cell line with a reporter, which was manipulated so that even beginners could easily culture it.
We also attempted to create a knockout cynomolgus monkey with a mutation at the targeted site, and succeeded in creating a genetically modified monkeys that lacked the PKD1 gene, which is the major causative gene of autosomal dominant polycystic kidney disease (ADPKD).

Academic Significance and Societal Importance of the Research Achievements

実験初心者でも容易に培養できるよう工夫したレポーター入りのカニクイザルES細胞株が樹立できたことで、今までサルES細胞を用いたことがない研究者でもサルES細胞を用いた研究がよりやりやすくなり研究の裾野を広げられると考えられる。
また、カニクイザルにおいて疾患モデルを作出することにより、マウスなどの小動物を用いた従来の疾患モデルよりも正確にヒトにおける病態を再現できることを示した。作製されたサルモデルは新しい治療戦略を確立するための技術基盤となることが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (16 results)

All 2020 2019 2018 2017 Other

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Generation of transgenic cynomolgus monkeys overexpressing the gene for amyloid-β precursor protein2020

    • Author(s)
      Seita Y, Morimura T, Watanabe N, Iwatani C, Tsuchiya H, Nakamura S, Suzuki T, Yanagisawa D, Tsukiyama T, Nakaya M, Okamura E, Muto M, Ema M, Nishimura M, Tooyama I.
    • Journal Title

      Journal of Alzheimer's Disease

      Volume: - Issue: 1 Pages: 45-60

    • DOI

      10.3233/jad-191081

    • NAID

      120006859791

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Induction of the germ cell fate from pluripotent stem cells in cynomolgus monkeys.2020

    • Author(s)
      Sakai Y, Nakamura T, Okamoto I, Gyobu-Motani S, Ohta H, Yabuta Y, Tsukiyama T, Iwatani C, Tsuchiya H, Ema M, Morizane A, Takahashi J, Yamamoto T, Saitou M.
    • Journal Title

      Biol Reprod

      Volume: 102(3) Issue: 3 Pages: 620-638

    • DOI

      10.1093/biolre/ioz205

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease2019

    • Author(s)
      Tsukiyama T, Kobayashi K, Nakaya M, Iwatani C, Seita Y, Tsuchiya H, Matsushita J, Kitajima K, Kawamoto I, Nakagawa T, Fukuda K, Iwakiri T, Izumi H, Itagaki I, Kume S, Maegawa H, Nishinakamura R, Nishio, Nakamura S, Kawauchi A, Ema M
    • Journal Title

      Nature Communications

      Volume: 10 (1) Issue: 1 Pages: 5517-5517

    • DOI

      10.1038/s41467-019-13398-6

    • NAID

      120006773159

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.2019

    • Author(s)
      Kobayashi K, Tsukiyama T, Nakaya M, Kageyama S, Tomita K, Murai R, Yoshida T, Narita M, Kawauchi A, Ema M.
    • Journal Title

      Stem cell research

      Volume: Epub Pages: 101439-101439

    • DOI

      10.1016/j.scr.2019.101439

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†2019

    • Author(s)
      Seita Yasunari、Tsukiyama Tomoyuki、Azami Takuya、Kobayashi Kenichi、Iwatani Chizuru、Tsuchiya Hideaki、Nakaya Masataka、Tanabe Hideyuki、Hitoshi Seiji、Miyoshi Hiroyuki、Nakamura Shinichiro、Kawauchi Akihiro、Ema Masatsugu
    • Journal Title

      Biology of Reproduction

      Volume: 印刷中 Issue: 6 Pages: 1440-1452

    • DOI

      10.1093/biolre/ioz040

    • NAID

      120006594188

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells2019

    • Author(s)
      Watanabe C., Matsushita J., Azami T., Tsukiyama-Fujii S., Tsukiyama T., Mizuno S., Takahashi S., and Ema M.
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 1 Pages: e0210060-e0210060

    • DOI

      10.1371/journal.pone.0210060

    • NAID

      120007128325

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis2017

    • Author(s)
      Arata Honda, Narantsog Choijookhuu, Haruna Izu, Yoshihiro Kawano, Mizuho Inokuchi, Kimiko Honsho, Ah-Reum Lee, Hiroki Nabekura, Hiroshi Ohta, Tomoyuki Tsukiyama, Yasuhide Ohinata, Asato Kuroiwa, Yoshitaka Hishikawa, Mitinori Saitou, Takamichi Jogahara, Chihiro Koshimoto
    • Journal Title

      Science Advances

      Volume: - Issue: 5

    • DOI

      10.1126/sciadv.1602179

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Disease modeling in cynomolgus monkeys using CRISPR/Cas9.2020

    • Author(s)
      Tsukiyama T.
    • Organizer
      日本発生生物学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] CRISPR/Cas9を用いたカニクイザルにおける疾患モデリング2020

    • Author(s)
      築山 智之
    • Organizer
      日本マーモセット研究会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] カニクイザルを用いた遺伝性腎疾患動物モデルの作製2018

    • Author(s)
      小林 憲市, 築山 智之, 影山 進, 岩谷 千鶴, 福田 浩司, 岩切 哲平, 和泉 博之,中村 紳一朗, 依馬 正次, 河内 明宏
    • Organizer
      日本泌尿器科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] CRISPR/Cas9を用いたADPKDモデルカニクイザルの作出と解析2018

    • Author(s)
      依馬 正次, 築山 智之, 小林 憲市, 和泉 博之, 河内 明宏
    • Organizer
      日本腎臓学会東部学術大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] CRISPR/Cas9を用いた遺伝性腎疾患モデルカニクイザルの作出2018

    • Author(s)
      築山 智之, 小林 憲市, 中家 雅隆, 岩谷 千鶴, 土屋 英明, 清田 弥寿成, 松下 淳, 北嶋 郁, 河本 育士, 中川 孝博, 福田 浩司, 岩切 哲平, 和泉 博之, 板垣 伊織, 中村 紳一朗, 河内 明宏, 依馬 正次
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Disease modeling in cynomolgus monkeys using CRISPR/Cas9 injection into ICSI embryos2017

    • Author(s)
      Tsukiyama T., Kobayashi K., Iwatani C., Tsuchiya H., Seita Y., Matsushita J., Kitajima K., Kawamoto I., Nakagawa T., Fukuda K., Iwakiri T., Izumi H., Itagaki I., Nakamura S., Kawauchi A. and Ema M.
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] CRISPR/Cas9を用いた疾患モデルカニクイザルの作出2017

    • Author(s)
      築山 智之
    • Organizer
      関西血管生物研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] CRISPR/Cas9を用いた疾患モデルカニクイザルの作出2017

    • Author(s)
      築山 智之, 小林 憲市, 岩谷 千鶴, 土屋 英明, 清田 弥寿成, 松下 淳, 北嶋 郁, 河本 育士, 中川 孝博, 福田 浩司, 岩切 哲平, 和泉 博之, 板垣 伊織, 中村 紳一朗, 河内 明宏, 依馬 正次
    • Organizer
      生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Remarks] 滋賀医科大学 動物生命科学研究センター 幹細胞・ヒト疾患モデル研究分野

    • URL

      http://lab.rcals.jp/

    • Related Report
      2018 Research-status Report 2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi