Establishment of high-quality novel ES / iPS cells that precisely reflect the in vivo state
Project/Area Number |
17K08135
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Integrative animal science
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Research Institution | Shiga University of Medical Science |
Principal Investigator |
TSUKIYAMA Tomoyuki 滋賀医科大学, 動物生命科学研究センター, 特任准教授 (60612132)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | iPS細胞 / ES細胞 / カニクイザル / 霊長類 / ゲノム編集 / CRISPR/Cas9 / 多能性幹細胞 |
Outline of Final Research Achievements |
We generated reporters for establishing high-quality novel human and cynomolgus monkey ES / iPS cells that precisely reflect the in vivo state, and performed reporter knock-in on cynomolgus monkey ES cells. In this process, we established a cynomolgus monkey ES cell line with a reporter, which was manipulated so that even beginners could easily culture it. We also attempted to create a knockout cynomolgus monkey with a mutation at the targeted site, and succeeded in creating a genetically modified monkeys that lacked the PKD1 gene, which is the major causative gene of autosomal dominant polycystic kidney disease (ADPKD).
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Academic Significance and Societal Importance of the Research Achievements |
実験初心者でも容易に培養できるよう工夫したレポーター入りのカニクイザルES細胞株が樹立できたことで、今までサルES細胞を用いたことがない研究者でもサルES細胞を用いた研究がよりやりやすくなり研究の裾野を広げられると考えられる。 また、カニクイザルにおいて疾患モデルを作出することにより、マウスなどの小動物を用いた従来の疾患モデルよりも正確にヒトにおける病態を再現できることを示した。作製されたサルモデルは新しい治療戦略を確立するための技術基盤となることが期待される。
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Generation of transgenic cynomolgus monkeys overexpressing the gene for amyloid-β precursor protein2020
Author(s)
Seita Y, Morimura T, Watanabe N, Iwatani C, Tsuchiya H, Nakamura S, Suzuki T, Yanagisawa D, Tsukiyama T, Nakaya M, Okamura E, Muto M, Ema M, Nishimura M, Tooyama I.
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Journal Title
Journal of Alzheimer's Disease
Volume: -
Issue: 1
Pages: 45-60
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Induction of the germ cell fate from pluripotent stem cells in cynomolgus monkeys.2020
Author(s)
Sakai Y, Nakamura T, Okamoto I, Gyobu-Motani S, Ohta H, Yabuta Y, Tsukiyama T, Iwatani C, Tsuchiya H, Ema M, Morizane A, Takahashi J, Yamamoto T, Saitou M.
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Journal Title
Biol Reprod
Volume: 102(3)
Issue: 3
Pages: 620-638
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease2019
Author(s)
Tsukiyama T, Kobayashi K, Nakaya M, Iwatani C, Seita Y, Tsuchiya H, Matsushita J, Kitajima K, Kawamoto I, Nakagawa T, Fukuda K, Iwakiri T, Izumi H, Itagaki I, Kume S, Maegawa H, Nishinakamura R, Nishio, Nakamura S, Kawauchi A, Ema M
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Journal Title
Nature Communications
Volume: 10 (1)
Issue: 1
Pages: 5517-5517
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis2017
Author(s)
Arata Honda, Narantsog Choijookhuu, Haruna Izu, Yoshihiro Kawano, Mizuho Inokuchi, Kimiko Honsho, Ah-Reum Lee, Hiroki Nabekura, Hiroshi Ohta, Tomoyuki Tsukiyama, Yasuhide Ohinata, Asato Kuroiwa, Yoshitaka Hishikawa, Mitinori Saitou, Takamichi Jogahara, Chihiro Koshimoto
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Journal Title
Science Advances
Volume: -
Issue: 5
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] CRISPR/Cas9を用いた遺伝性腎疾患モデルカニクイザルの作出2018
Author(s)
築山 智之, 小林 憲市, 中家 雅隆, 岩谷 千鶴, 土屋 英明, 清田 弥寿成, 松下 淳, 北嶋 郁, 河本 育士, 中川 孝博, 福田 浩司, 岩切 哲平, 和泉 博之, 板垣 伊織, 中村 紳一朗, 河内 明宏, 依馬 正次
Organizer
日本分子生物学会
Related Report
Invited
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[Presentation] Disease modeling in cynomolgus monkeys using CRISPR/Cas9 injection into ICSI embryos2017
Author(s)
Tsukiyama T., Kobayashi K., Iwatani C., Tsuchiya H., Seita Y., Matsushita J., Kitajima K., Kawamoto I., Nakagawa T., Fukuda K., Iwakiri T., Izumi H., Itagaki I., Nakamura S., Kawauchi A. and Ema M.
Organizer
4th World Congress of Reproductive Biology
Related Report
Int'l Joint Research
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[Presentation] CRISPR/Cas9を用いた疾患モデルカニクイザルの作出2017
Author(s)
築山 智之, 小林 憲市, 岩谷 千鶴, 土屋 英明, 清田 弥寿成, 松下 淳, 北嶋 郁, 河本 育士, 中川 孝博, 福田 浩司, 岩切 哲平, 和泉 博之, 板垣 伊織, 中村 紳一朗, 河内 明宏, 依馬 正次
Organizer
生命科学系学会合同年次大会
Related Report
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