Development of tree-dimensional MS imaging for sphingolipids
| Project/Area Number |
17K08233
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
Saigusa Daisuke 東北大学, 東北メディカル・メガバンク機構, 講師 (90545237)
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| Co-Investigator(Kenkyū-buntansha) |
可野 邦行 東北大学, 薬学研究科, 助教 (50636404)
元池 育子 東北大学, 東北メディカル・メガバンク機構, 准教授 (70347178)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | スフィンゴ脂質 / MALDI / MSイメージング / 質量分析計 / S1P / 凍結組織切片 / 粘着性導電性フィルム / イメージングMS / 組織切片 / 立体可視化 / DESI / DIUTHAME / IMS / スフィンゴシン一リン酸 / イオンモビリティ / イメージング MS / LC-MS/MS / 肝癌 |
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| Outline of Final Research Achievements |
Sphingolipids are bioactive lipid mediators related with the expression of cancer and immune disease and has been estimated the target molecules of drug discovery. Although their biological functions were reacted beside on the metabolism-related molecules on tissue, they could not be detected directly on the tissue section in previous studies. In this study, we demonstrated that the conductive adhesive film can overcome the setbacks of the MALDI-MSI approach by allowing the imaging of intact cryosections, such as the bone, muscle, adipose tissues and whole mouse body. We finally developed the on-tissue derivatization method for the detection of S1P on the cryosection and improved the ion intensities of sphingolipids by means of the MALDI-MSI analysis.
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| Academic Significance and Societal Importance of the Research Achievements |
スフィンゴ脂質の一種であるS1Pは、脂質メディエーター機能が注目されているにも関わらず、これまでに組織切片上で直接検出することはできなかったため、研究の大きな障壁となっていた。我々は、世界で初めてMALDI-MSイメージングによるS1Pの可視化に成功したことから、今後のS1Pを標的とした基礎研究および創薬開発に大きな発展をもたらすことから、極めて社会的意義が高い。また、導電性粘着フィルムは、S1Pやスフィンゴ脂質のみならず各種代謝物について、これまでに凍結切片作成が困難であった組織におけるMALDI-MSIによる解析が可能になることから学術的な意義は極めて高い。
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Alteration of the lysophosphatidic acid and its precursor lysophosphatidylcholine levels in spinal cord stenosis: A study using a rat cauda equina compression model.2019
Author(s)
Uranbileg B, Ito N, Kurano M, Saigusa D, Saito R, Uruno A, Kano K, Ikeda H, Yamada Y, Sumitani M, Sekiguchi M, Aoki J, Yatomi Y.
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Journal Title
Scientific reports
Volume: 9
Issue: 1
Pages: 16578-16578
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Metabolomic changes in the mouse retina after optic nerve injury.2018
Author(s)
Sato K, Saigusa D, Saito R, Fujioka A, Nakagawa Y, Nishiguchi KM, Kokubun T, Motoike IN, Maruyama K, Omodaka K, Shiga Y, Uruno A, Koshiba S, Yamamoto M, Nakazawa T.
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Journal Title
Sci Rep
Volume: 8
Issue: 1
Pages: 11930-11930
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Intracellular S1P Levels Dictate Fate of Different Regions of the Hippocampus following Transient Global Cerebral Ischemia.2018
Author(s)
Rashad S, Niizuma K, Saigusa D, Han X, Sato-Maeda M, Saito R, Uruno A, Fujimura M, Ikawa S, Yamamoto M, Tominaga T.
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Journal Title
Neuroscience
Volume: 384
Pages: 188-202
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Impact of the Oral Adsorbent AST-120 on organ-specific accumulation of uremic toxins: LC-MS/MS and MS imaging techniques2018
Author(s)
Emiko Sato, Daisuke Saigusa, Eikan Mishima, Taeko Uchida, Daisuke Miura, Tomomi Morikawa-Ichinose, Kiyomi Kisu, Akiyo Sekimoto, Ritsumi Saito, Yuji Oe, Yotaro Matsumoto, Yoshihisa Tomioka, Takefumi Mori, Nobuyuki Takahashi, Hirosi Sato, Takaaki Abe, Toshimitsu Niwa, and Sadayosi Ito
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Journal Title
Toxins
Volume: 10
Issue: 1
Pages: 19-33
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Evidence Suggests Sphingosine 1-Phosphate Might Be Actively Generated, Degraded, and Transported to Extracellular Spaces With Increased S1P2 and S1P3 Expression in Colon Cancer.2017
Author(s)
Uranbileg B, Nishikawa T, Ikeda H, Kurano M, Sato M, Saigusa D, Aoki J, Watanabe T, Yatomi Y.
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Journal Title
Clin Colorectal Cancer.
Volume: Nov 21. pii:
Issue: 2
Pages: 30369-9
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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