• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of micro total analysis system for biological compounds

Research Project

Project/Area Number 17K08234
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

Tsunoda Makoto  東京大学, 大学院薬学系研究科(薬学部), 准教授 (10323453)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsクロマトグラフィー / マイクロ流体デバイス / マイクロ化学チップ / チオール / マイクロ化学分析システム / 蛍光
Outline of Final Research Achievements

A technology to enable quantification of trace amounts of biological compounds using pillar array columns as a high-performance on-chip liquid chromatography separation medium was developed. So far, fundamental technologies for separation of biological compounds on pillar array columns have been established. In this study, the sample injection part was investigated as one of the fundamental technologies for quantitative analysis. An automated sample injection system was developed with good reproducibility. Reproducible quantification of amino acids in plasma samples has become possible.

Academic Significance and Societal Importance of the Research Achievements

オンチップ液体クロマトグラフィー技術を用いた生体試料中低分子化合物定量への応用は、これまで、限定されていた。本研究成果により、オンチップ液体クロマトグラフィーの中でも高性能な分離が可能であるピラーアレイカラムを用いた生体分子定量が可能となった。ピラーアレイカラムは高分離能を有することから、生体分子の迅速分析による疾病診断等への応用が期待される。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (27 results)

All 2021 2020 2019 2018 2017

All Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results,  Open Access: 8 results) Presentation (16 results) (of which Int'l Joint Research: 10 results,  Invited: 10 results)

  • [Journal Article] Quantification of Intracellular Thiols by HPLC-Fluorescence Detection2021

    • Author(s)
      Yamamoto, Hiroki, Takuya Fujiwara, Takashi Funatsu, and Makoto Tsunoda
    • Journal Title

      Molecules

      Volume: 26 Issue: 8 Pages: 2365-2365

    • DOI

      10.3390/molecules26082365

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fast analysis using pillar array columns: Quantification of branched-chain α-keto acids in human plasma samples2021

    • Author(s)
      Takuya Fujiwara, Takashi Funatsu, Makoto Tsunoda
    • Journal Title

      J Pharm Biomed Anal

      Volume: 198 Pages: 114019-114019

    • DOI

      10.1016/j.jpba.2021.114019

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an Automated Sample Injection System for Pillar Array Columns2020

    • Author(s)
      KUROKI Hiroshi、KOYAMA Hirotaka、NAKATANI Yusuke、FUNATSU Takashi、HORIIKE Shigeyoshi、TSUNODA Makoto
    • Journal Title

      CHROMATOGRAPHY

      Volume: 41 Issue: 1 Pages: 59-62

    • DOI

      10.15583/jpchrom.2019.022

    • NAID

      130007807228

    • ISSN
      1342-8284, 1348-3315
    • Year and Date
      2020-02-20
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of intracellular a-keto acids by HPLC with fluorescence detection2020

    • Author(s)
      Takuya Fujiwara, Ayuna Hattori, Takahiro Ito, Takashi Funatsu, Makoto Tsunoda
    • Journal Title

      Anal. Methods

      Volume: 12 Issue: 20 Pages: 2555-2559

    • DOI

      10.1039/d0ay00556h

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Liquid-Chromatographic Methods for Carboxylic Acids in Biological Samples2020

    • Author(s)
      Takuya Fujiwara, Ryoto Inoue, Takuma Ohtawa, Makoto Tsunoda
    • Journal Title

      Molecules

      Volume: 25 Issue: 21 Pages: 4883-4883

    • DOI

      10.3390/molecules25214883

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples2019

    • Author(s)
      Chang Chunfang、Isokawa Muneki、Funatsu Takashi、Tsunoda Makoto
    • Journal Title

      Heliyon

      Volume: 5 Issue: 5 Pages: e01598-e01598

    • DOI

      10.1016/j.heliyon.2019.e01598

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Retention of Fluorescent Amino Acid Derivatives in Ion-pairing Reversed-phase Liquid Chromatography2018

    • Author(s)
      Li X, Kuroki H, Funatsu T, Tsunoda M
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 10 Pages: 1209-1212

    • DOI

      10.2116/analsci.18N008

    • NAID

      130007496866

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2018-10-10
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of methylated tubulin through analysis of methylated lysine.2018

    • Author(s)
      Suzuki R, Funatsu T, Tsunoda M
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 410 Issue: 17 Pages: 4189-4194

    • DOI

      10.1007/s00216-018-1068-9

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cancer progression by reprogrammed BCAA metabolism in myeloid leukaemia2017

    • Author(s)
      Hattori Ayuna、Tsunoda Makoto、Konuma Takaaki、Kobayashi Masayuki、Nagy Tamas、Glushka John、Tayyari Fariba、McSkimming Daniel、Kannan Natarajan、Tojo Arinobu、Edison Arthur S.、Ito Takahiro
    • Journal Title

      Nature

      Volume: 545 Issue: 7655 Pages: 500-504

    • DOI

      10.1038/nature22314

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column2017

    • Author(s)
      SUMIDA Yuko、TSUNODA Makoto、FUNATSU Takashi
    • Journal Title

      CHROMATOGRAPHY

      Volume: 38 Issue: 3 Pages: 135-138

    • DOI

      10.15583/jpchrom.2017.016

    • NAID

      130007040472

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of Branched-Chain Keto Acids in Cell Extracts by HPLC-Fluorescence Detection2017

    • Author(s)
      HATTORI Ayuna、ITO Takahiro、TSUNODA Makoto
    • Journal Title

      CHROMATOGRAPHY

      Volume: 38 Issue: 3 Pages: 129-133

    • DOI

      10.15583/jpchrom.2017.010

    • NAID

      130007040468

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Analysis of biological compounds using pillar array columns2021

    • Author(s)
      Makoto Tsunoda
    • Organizer
      the 31st International Symposium on Pharmaceutical and Biomedical Analysis (PBA2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 3rd International Conference on Analytical and Bioanalytical Methods2021

    • Author(s)
      Makoto Tsunoda
    • Organizer
      Quantitative Analysis of Biological Compounds Using a Pillar Array Column
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] オンチップ液体クロマトグラフィーを用いた生体分子の定量分析2021

    • Author(s)
      角田 誠
    • Organizer
      クロマトグラフィー次世代技術セミナー2021
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] オンチップ液体クロマトグラフィーによる生体分子分析2021

    • Author(s)
      角田 誠
    • Organizer
      第32回クロマトグラフィー科学会議
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] オンチップLCによるα-ケト酸の迅速分析法の開発2020

    • Author(s)
      藤原 拓哉、船津 高志、角田 誠
    • Organizer
      第20回東京大学生命科学シンポジウム
    • Related Report
      2020 Research-status Report
  • [Presentation] Analysis of Branched-Chain Amino Acids Using On-chip Liquid Chromatography2020

    • Author(s)
      Makoto Tsunoda
    • Organizer
      Analyticon-2020
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] LC チップにおける定量分析に向けた試料自動注入システムの開発2019

    • Author(s)
      黒木 啓志、船津 高志、角田 誠
    • Organizer
      第32回 バイオメディカル分析科学シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] Development of automated sample injection system for pillar array columns2019

    • Author(s)
      Hiroshi Kuroki, Takashi Funatsu, Makoto Tsunoda
    • Organizer
      49th International Symposium on High Performance Liquid Phase Separations and Related Techniques
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ピラーアレイカラムのための試料自動注入システムの開発2019

    • Author(s)
      黒木 啓志、船津 高志、角田 誠
    • Organizer
      新アミノ酸分析研究会第9回学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] Analysis of biological compounds by hydrophilic interaction chromatography2019

    • Author(s)
      Makoto Tsunoda
    • Organizer
      The 7th Symposium of the Division of Pharmaceutical Analysis for the Pharmaceutical Society of Korea
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Chip-Based Liquid Chromatography Analysis for Biological Compounds2018

    • Author(s)
      Makoto Tsunoda
    • Organizer
      2018 Sino-Japanese Joint Symposium on Separation Science
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Chip-Based Liquid Chromatography Analysis for Biological Compounds2018

    • Author(s)
      Makoto Tsunoda
    • Organizer
      32nd International Symposium on Chromatography
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] オンチップ液体クロマトグラフィーの開発と生体分子分析への応用2018

    • Author(s)
      角田誠
    • Organizer
      日本薬学会東海支部講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] On-chip Liquid Chromatography for Analysis of Biological Compounds2018

    • Author(s)
      Makoto Tsunoda
    • Organizer
      The 6th Winter Symposium of the Division of Pharmaceutical Analysis for the Pharmaceutical Society of Korea
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] One-minute separation of five biothiols using amide-modified liquid chromatography chip2017

    • Author(s)
      Makoto Tsunoda, Muneki Isokawa, Kanki Nakanishi, Takashi Funatsu, Shuichi Shoji
    • Organizer
      HPLC2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] HILIC Analysis of Biological Compounds2017

    • Author(s)
      Makoto Tsunoda
    • Organizer
      HPLC2017 Jeju
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi