Fabrication of polymer nano-film possessing hydrophobic and hydrophilic surface and its application to DDS

• Project/Area Number: 17K08242
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: Kondo Shin-ichi 岐阜薬科大学, 薬学部, 教授 (90240944)
• Co-Investigator(Kenkyū-buntansha): 笹井 泰志 岐阜薬科大学, 薬学部, 准教授 (60336633) ; 土井 直樹 岐阜薬科大学, 薬学部, 助教 (00781436)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 高分子ナノフィルム / リン脂質膜自己組織化膜 / 架橋反応 / 動的光散乱測定 / 原子間力顕微鏡 / ｐH応答性 / リン脂質自己組織化膜 / 薬物放出 / ドラッグデリバリー / 自己組織化膜 / プラズマ加工

Outline of Final Research Achievements

In this study we aimed to fabricate the self-standing amphiphilic polymer nano-film, which possessed both hydrophilic and hydrophobic side, on the self-assembled phospholipid layer having fluidity. We examined the particle size and shape of the amphiphilic polymer nano-film which was synthesized under various experimental conditions. It was revealed that the particle size of polymer nano-film could be controlled by the experimental condition (temperature and reaction time). It was also shown that the solvent could affect the shape of the polymer nano-film. The drug release profile from the polymer nano-film at pH 5.0 was discussed. The particle diameter at pH 5.0 was bigger than that at pH 7.4, due to the electric repulsion among amino groups which was protonated at pH 5.0. It was suggested that the drug release from the polymer nano-film could be induced by the increase of the particle diameter at pH 5.0.

Academic Significance and Societal Importance of the Research Achievements

本研究では、有機物のみからなる親水性面と疎水性面を持った高分子ナノフィルムの開発とその薬物放出特性について明らかにした。従来の高分子ナノフィルムは、有機材料と金属イオンから成るものであり、かつ両面とも同じ構造あるいは物性である。したがって、本方法により得られる高分子ナノフィルムは、既存のものよりも多様性が高く、原理的には親水性面と疎水性面だけでなく異なる官能基を導入した面を構築することも可能となり様々な応用への展開も期待される。さらに、ｐH応答性などの刺激応答性機能を本高分子ナノフィルムに導入することによりより広範な利用が期待される。

Research Products

• [Journal Article] Application to Nano Drug Carrier Using Polymer Nano-Film Synthesized on Self-Assembled Phospholipid Layer Fabricated by Plasma-Assisted Method (2019)
  • Author(s): S. Kondo, Y. Sasai, N. Doi, Y. Yamauchi, and M. Kuzuya
  • Journal Title: Journal of Photopolymer Science and Technology Volume: 32 Issue: 3 Pages: 541-544
  • DOI: 10.2494/photopolymer.32.541
  • NAID: 130007744422
  • ISSN: 0914-9244, 1349-6336
  • Year and Date: 2019-06-24
  • Peer Reviewed
• [Journal Article] Fundamental Study on Development of Polymer Nano-Film Synthesized on Self-Assembled Phospholipid Layer Fabricated by Plasma-Assisted Method (2018)
  • Author(s): S. Kondo, Y. Sasai, Y. Yamauchi, M. Kuzuya
  • Journal Title: Journal of Photopolymer Science and Technology Volume: 31 Issue: 3 Pages: 385-388
  • DOI: 10.2494/photopolymer.31.385
  • NAID: 130007481484
  • ISSN: 0914-9244, 1349-6336
  • Year and Date: 2018-06-25
  • Peer Reviewed
• [Presentation] プラズマを利用して構築した高分子ナノフィルムの薬物キャリヤーとしての応用 (2019)
  • Author(s): 近藤 伸一、笹井 泰志、土井 直樹、山内 行玄、葛谷 昌之
  • Organizer: 第36回国際フォトポリマーコンファレンス
  • Int'l Joint Research
• [Presentation] Development of Polymer Nano-Film Synthesized on Self-Assembled Phospholipid Layer Fabricated by Plasma-Assisted Method (2019)
  • Author(s): S. Kondo, Y. Sasai, N. Doi, Y. Yamauchi, M. Kuzuya
  • Organizer: Materials Research Meeting 2019
  • Int'l Joint Research / Invited
• [Presentation] プラズマを利用して構築したリン脂質膜を用いる高分子ナノフィルム開発の基礎研究 (2018)
  • Author(s): 近藤伸一、笹井泰志、山内行玄、葛谷昌之
  • Organizer: 第35回国際フォトポリマーコンファレンス
  • Int'l Joint Research