Elucidation of the relationship between onset of diabetes and survival strategies of enterobacteria that alter intestinal nutritional status

• Project/Area Number: 17K08288
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Meiji Pharmaceutical University (2019); Teikyo University (2017-2018)
• Principal Investigator: Matsumoto Yasuhiko 明治薬科大学, 薬学部, 准教授 (60508141)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: カイコ / 食後高血糖 / 糖尿病 / 乳酸菌 / 腸内細菌 / 高血糖 / グルコース / 分子生物学 / 細菌学

Outline of Final Research Achievements

Postprandial hyperglycemia causes lifestyle-related diseases such as diabetes and obesity. In this study, we identified Enterococcus faecalis YM0831 strain that suppresses postprandial hyperglycemia in silkworms. E. faecalis YM0831 inhibited glucose permeability in the isolated intestinal tract of silkworm and glucose uptake by Caco-2 cells, derived from the human intestine. In a human clinical trial, E. faecalis YM0831 suppressed an increase in blood glucose level in a sucrose tolerance test. These results suggest that E. faecalis YM0831 inhibits sugar uptake in human intestine and prevents postprandial hyperglycemia in humans.

Academic Significance and Societal Importance of the Research Achievements

カイコの食後高血糖モデルが機能性乳酸菌の同定に有効であることがわかった。カイコを用いたin vivoスクリーニングにより同定された物質がヒトに対しても同様に有効であることを示した初めての研究であり、カイコの実験系がヒトの健康に貢献する物質の同定に有効であることを示している。さらに、E. faecalis YM0831株を用いて製造したヨーグルトもヒトのショ糖摂取後の血糖値の上昇を抑制することも明らかとなった。このヨーグルトを食前に摂取することで食後高血糖を抑制し、糖尿病などの生活習慣病の予防に貢献できることが期待される。

Research Products

• [Journal Article] Suppressive effects of whey protein hydrolysate on sucrose-induced hyperglycemia in silkworms (2019)
  • Author(s): Matsumoto Yasuhiko、Takahashi Miki、Umehara Masahiro、Asano Masato、Maruki-Uchida Hiroko、Morita Minoru、Sekimizu Kazuhisa
  • Journal Title: Drug Discoveries & Therapeutics Volume: 13 Issue: 5 Pages: 244-247
  • DOI: 10.5582/ddt.2019.01069
  • NAID: 130007744553
  • ISSN: 1881-7831, 1881-784X
  • Year and Date: 2019-10-31
  • Peer Reviewed / Open Access
• [Journal Article] Additive effects of Kothala himbutu (<i>Salacia reticulata</i>) extract and a lactic acid bacterium (<i>Enterococcus faecalis</i> YM0831) for suppression of sucrose-induced hyperglycemia in an <i>in vivo </i>silkworm evaluation system (2019)
  • Author(s): Ishii Masaki、Matsumoto Yasuhiko、Katada Toshiaki、Sekimizu Kazuhisa
  • Journal Title: Drug Discoveries & Therapeutics Volume: 13 Issue: 3 Pages: 133-136
  • DOI: 10.5582/ddt.2019.01025
  • NAID: 130007682072
  • ISSN: 1881-7831, 1881-784X
  • Year and Date: 2019-06-30
  • Peer Reviewed / Open Access
• [Journal Article] Enterococcus faecalis YM0831 suppresses sucrose-induced hyperglycemia in a silkworm model and in humans (2019)
  • Author(s): Matsumoto Yasuhiko、Ishii Masaki、Hasegawa Setsuo、Sekimizu Kazuhisa
  • Journal Title: Communications Biology Volume: 2 Issue: 1 Pages: 157-157
  • DOI: 10.1038/s42003-019-0407-5
  • Peer Reviewed / Open Access
• [Journal Article] Silkworm as an experimental animal for research on fungal infections (2019)
  • Author(s): Matsumoto Yasuhiko、Sekimizu Kazuhisa
  • Journal Title: Microbiology and Immunology Volume: 63 Issue: 2 Pages: 41
  • DOI: 10.1111/1348-0421.12668
  • NAID: 40021840652
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a gnotobiotic silkworm model (2018)
  • Author(s): Nakajima H, Matsumoto Y, Sekimizu K
  • Journal Title: Drug Discoveries & Therapeutics Volume: 12 Issue: 5 Pages: 291-294
  • DOI: 10.5582/ddt.2018.01048
  • NAID: 130007521061
  • ISSN: 1881-7831, 1881-784X
  • Year and Date: 2018-10-31
  • Peer Reviewed / Open Access
• [Journal Article] Bacterial polysaccharides inhibit sucrose-induced hyperglycemia in silkworms (2018)
  • Author(s): Ishii M, Matsumoto Y, Sekimizu K
  • Journal Title: Drug Discoveries & Therapeutics Volume: 12 Issue: 4 Pages: 185-188
  • DOI: 10.5582/ddt.2018.01040
  • NAID: 130007484445
  • ISSN: 1881-7831, 1881-784X
  • Year and Date: 2018-08-31
  • Peer Reviewed / Open Access
• [Journal Article] Inhibitory effects of alpha-cyclodextrin and its derivative against sucrose-induced hyperglycemia in an <i>in vivo</i> evaluation system (2018)
  • Author(s): Ishii M, Matsumoto Y, Sekimizu K
  • Journal Title: Drug Discoveries & Therapeutics Volume: 12 Issue: 3 Pages: 122-125
  • DOI: 10.5582/ddt.2018.01028
  • NAID: 130007403869
  • ISSN: 1881-7831, 1881-784X
  • URL: https://pure.teikyo.jp/en/publications/f02ecf37-5d92-4844-aa33-3bbbbb188a47
  • Year and Date: 2018-06-30
  • Peer Reviewed / Open Access
• [Journal Article] Estimation of lactic acid bacterial cell number by DNA quantification (2018)
  • Author(s): Ishii M, Matsumoto Y, Sekimizu K
  • Journal Title: Drug Discoveries & Therapeutics Volume: 12 Issue: 2 Pages: 88-91
  • DOI: 10.5582/ddt.2018.01019
  • NAID: 130006734006
  • ISSN: 1881-7831, 1881-784X
  • Year and Date: 2018-04-30
  • Peer Reviewed / Open Access
• [Journal Article] D-cycloserine increases the effectiveness of vancomycin against vancomycin-highly resistant Staphylococcus aureus (2017)
  • Author(s): Tabuchi Fumiaki、Matsumoto Yasuhiko、Ishii Masaki、Tatsuno Keita、Okazaki Mitsuhiro、Sato Tomoaki、Moriya Kyoji、Sekimizu Kazuhisa
  • Journal Title: Journal of Antibiotics Volume: 70 Issue: 8 Pages: 907
  • DOI: 10.1038/ja.2017.56
  • Peer Reviewed / Open Access
• [Journal Article] An invertebrate infection model for evaluating anti-fungal agents against dermatophytosis (2017)
  • Author(s): Ishii Masaki、Matsumoto Yasuhiko、Yamada Tsuyoshi、Abe Shigeru、Sekimizu Kazuhisa
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12289-12289
  • DOI: 10.1038/s41598-017-12523-z
  • Peer Reviewed / Open Access
• [Journal Article] A Silkworm Infection Model to Evaluate Antifungal Drugs for Cryptococcosis (2017)
  • Author(s): Matsumoto Yasuhiko、Ishii Masaki、Shimizu Kiminori、Kawamoto Susumu、Sekimizu Kazuhisa
  • Journal Title: Medical Mycology Journal Volume: 58 Issue: 4 Pages: E131-E137
  • DOI: 10.3314/mmj.17.016
  • NAID: 130006233755
  • ISSN: 2185-6486, 2186-165X
  • Peer Reviewed / Open Access
• [Journal Article] Two-spotted cricket as an animal infection model of human pathogenic fungi (2017)
  • Author(s): Kochi Yuto、Matsumoto Yasuhiko、Sekimizu Kazuhisa、Kaito Chikara
  • Journal Title: Drug Discoveries & Therapeutics Volume: 11 Issue: 5 Pages: 259-266
  • DOI: 10.5582/ddt.2017.01052
  • NAID: 130006210824
  • ISSN: 1881-7831, 1881-784X
  • Peer Reviewed / Open Access
• [Journal Article] Silkworm fungal infection model for identification of virulence genes in pathogenic fungus and screening of novel antifungal drugs (2017)
  • Author(s): Ishii M, Matsumoto Y, Nakamura I, Sekimizu K
  • Journal Title: Drug Discoveries & Therapeutics Volume: 11 Issue: 1 Pages: 1-5
  • DOI: 10.5582/ddt.2016.01080
  • NAID: 130005466207
  • ISSN: 1881-7831, 1881-784X
  • URL: https://pure.teikyo.jp/en/publications/d3682f7d-6adc-4070-9475-4bffb4493da0
  • Peer Reviewed / Open Access
• [Journal Article] Decreased sugar concentration in vegetable and fruit juices by growth of functional lactic acid bacteria (2017)
  • Author(s): Ishii M, Matsumoto Y, Nishida S, Sekimizu K
  • Journal Title: Drug Discoveries & Therapeutics Volume: 11 Issue: 1 Pages: 30-34
  • DOI: 10.5582/ddt.2016.01079
  • NAID: 130005466206
  • ISSN: 1881-7831, 1881-784X
  • Peer Reviewed / Open Access
• [Presentation] eGFPを発現した病原性真菌Trichosporon asahiiを用いたカイコ感染モデルの確立 (2020)
  • Author(s): 松本靖彦、山﨑豪己、山﨑雄介、立山祐樹、山田剛、杉田隆
  • Organizer: 第93回日本細菌学会総会、愛知（ウィンクあいち）
  • Invited
• [Presentation] 真菌感染症研究のためのカイコの利用 (2019)
  • Author(s): 松本靖彦、関水和久
  • Organizer: 第92回日本細菌学会総会、北海道（札幌コンベンションセンター）
  • Invited
• [Presentation] 新しい実験動物を用いたショ糖摂取後の血糖値の上昇を抑制する機能性乳酸菌の同定法の確立 (2019)
  • Author(s): 松本靖彦
  • Organizer: 第13回細菌学若手コロッセウム、宮城（旬樹庵さんさ亭）
• [Presentation] 病原性真菌Trichosporon asahiiの感染システムを解明するためのカイコ感染モデルの開発 (2019)
  • Author(s): 松本靖彦、山﨑豪己、山﨑雄介、立山祐樹、山田剛、杉田隆
  • Organizer: 第31回微生物シンポジウム、京都（京都薬科大学）
• [Presentation] 病原性真菌Trichosporon asahiiのカイコ感染モデルを用いた抗真菌薬の評価系の確立 (2019)
  • Author(s): 松本靖彦、山﨑豪己、山﨑雄介、立山祐樹、山田剛、杉田隆
  • Organizer: 第102回日本細菌学会関東支部総会、長野（松本歯科大学）
• [Presentation] Silkworm as an experimental animal for research on fungal infections (2019)
  • Author(s): Yasuhiko Matsumoto
  • Organizer: 第63回日本医真菌学会総会・学術集会、千葉（オークラ千葉ホテル）
  • Invited
• [Presentation] “in vivo screening”を実現させるための感染動物モデルの構築と創薬への応用 (2019)
  • Author(s): 松本靖彦
  • Organizer: 2019年日本細菌学会関東支部インターラボセミナー -薬剤耐性菌の現状とアカデミアにおける新規抗菌薬の開発-
  • Invited
• [Presentation] New approach for drug discovery by using silkworm (2019)
  • Author(s): Yasuhiko Matsumoto
  • Organizer: 2nd International Conference on “Antimicrobial Resistance, Novel Drug Discovery and Development: Challenges and Opportunities”, Delhi RGEC campus Sonipat, India
  • Int'l Joint Research / Invited
• [Presentation] 経口抗白癬薬を評価するためのカイコ感染モデルの確立 (2018)
  • Author(s): 松本靖彦、石井雅樹、山田剛、安部茂、関水和久
  • Organizer: 第62回日本医真菌学会総会・学術集会、東京（大手町サンケイプラザ）
• [Presentation] クリプトコックス感染症に対する抗真菌薬のカイコを用いた評価 (2017)
  • Author(s): 松本靖彦、石井雅樹、清水公徳、川本進、関水和久
  • Organizer: 第23回真菌症フォーラム (東京：京王プラザホテル)
• [Presentation] カイコ白癬菌感染モデルを用いた白癬症治療薬の評価 (2017)
  • Author(s): 石井雅樹、松本靖彦、山田剛、安部茂、関水和久
  • Organizer: 第38回関東医真菌懇話会 (東京：京王プラザホテル)
• [Presentation] ショ糖摂取後の血糖上昇に対する乳酸菌YM0831株の抑制効果 (2017)
  • Author(s): 松本靖彦、石井雅樹、関水和久
  • Organizer: 第100回日本細菌学会関東支部会 (東京：帝京大学板橋キャンパス)
• [Presentation] 腸球菌YM0831は、ヒトにおけるショ糖摂取後の血糖値の上昇を抑制する (2017)
  • Author(s): 松本靖彦、石井雅樹、関水和久
  • Organizer: 第91回日本細菌学会総会 (福岡：福岡国際会議場)
• [Presentation] カイコを用いた経口抗白癬薬評価のための動物モデルの確立 (2017)
  • Author(s): 石井雅樹、松本靖彦、山田剛、安部茂、関水和久
  • Organizer: 第91回日本細菌学会総会 (福岡：福岡国際会議場)
• [Remarks] 松本靖彦 - 研究者 - researchmap
  • URL: https://researchmap.jp/Matsumon1980
• [Remarks] 明治薬科大学微生物学研究室
  • URL: http://www.focuto.ne.jp/mpu_dom/members.html
• [Remarks] 松本靖彦 - 研究者 - researchmap
  • URL: https://researchmap.jp/Matsumon1980/