• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Histological analysis of novel transforming mechanisms in adipocytes

Research Project

Project/Area Number 17K08508
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionYamagata University

Principal Investigator

Nakano Tomoyuki  山形大学, 医学部, 准教授 (00333948)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords脂肪組織 / ベージュ化 / ジアシルグリセロールキナーゼ / 高脂肪食 / 寒冷暴露 / 脂肪細胞 / 体温産生 / インスリン / 褐色化 / 体温 / 形質転換
Outline of Final Research Achievements

Diacylglycerol kinase (DGK) is an enzyme that converts DG to phosphatidic acid. DG is known as an intermediate product for triglyceride synthesis, but also is known as a lipidic second messenger. Thus, DG serves as a hub between energy metabolism and intracellular signaling. DGKe resides in the endoplasmic reticulum (ER). Since ER is known as a central site for lipid synthesis, current study focused on DGKe using its knock out mice. Two animal experiments were conducted in this project. One experiment revealed that beige adipogenesis was induced in the epididymal white adipose tissue(WAT) of DGKe-KO mice under long term High fat diet(HFD)) feeding. The other experiment showed that DGKe-KO mice was susceptible to cold exposure. To compensate body heat loss, beige adipogenesis was induced in inguinal WAT. Present projected concluded that stress conditions in adipose tissue such as HFD feeding and cold exposure, DGKe-deletion confers susceptibility to adipose tissue remodeling.

Academic Significance and Societal Importance of the Research Achievements

白色脂肪組織中に褐色脂肪細胞が誘導される褐色化は、肥満治療の観点で注目を集めているが、脂肪細胞の形質転換メカニズムという基礎的な、解剖学的に興味深い現象である。本研究プロジェクトの結果から、高エネルギー条件や寒冷環境において、DGKe欠損によるDG代謝の低下が褐色化を促進するという新規メカニズムが明らかとなった。特に、高エネルギー条件においては、DGKeと同じく小胞体に局在するシクロオキシゲナーゼ2依存的に褐色化が生じることは、小胞体における脂質代謝が褐色化に影響を与える点で興味深い知見である。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (24 results)

All 2022 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] ユタ大学(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Diacylglycerol Kinase ε in Adipose Tissues: A Crosstalk Between Signal Transduction and Energy Metabolism2022

    • Author(s)
      Nakano Tomoyuki、Goto Kaoru
    • Journal Title

      Frontiers in Physiology

      Volume: 13 Pages: 815085-815085

    • DOI

      10.3389/fphys.2022.815085

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cellular expression and subcellular localization of diacylglycerol kinase γ in rat brain2021

    • Author(s)
      Hozumi Y, Nakano T, Goto K.
    • Journal Title

      Biomedical Research

      Volume: 42 Issue: 1 Pages: 33-42

    • DOI

      10.2220/biomedres.42.33

    • NAID

      130007983453

    • ISSN
      0388-6107, 1880-313X
    • Year and Date
      2021-02-10
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of p53 and NF-κB transactivation activities by DGKζ in catalytic activity-dependent and -independent manners2021

    • Author(s)
      Tanaka Toshiaki、Nakano Tomoyuki、Hozumi Yasukazu、Martelli Alberto M.、Goto Kaoru
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

      Volume: 1868 Issue: 4 Pages: 118953-118953

    • DOI

      10.1016/j.bbamcr.2021.118953

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mice lacking DGKepsilon show increased beige adipogenesis in visceral WAT after long-term high fat diet in a COX-2-dependent manner.2020

    • Author(s)
      Nakano T., Topham M.K. and Goto K.
    • Journal Title

      Advances in Biological Regulation

      Volume: In press Pages: 100659-100659

    • DOI

      10.1016/j.jbior.2019.100659

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Deletion of diacylglycerol kinase ε confers susceptibility to obesity via reduced lipolytic activity in murine adipocytes2018

    • Author(s)
      Nakano T, Seino K, Wakabayashi I, Stafforini DM, Topham MK, Goto K
    • Journal Title

      FASEB J.

      Volume: - Issue: 8 Pages: 4121-4130

    • DOI

      10.1096/fj.201701050r

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] DgMab-6: Anti-Human DGK gamma Monoclonal Antibody for immunocytochemistry2018

    • Author(s)
      Nakano T., Ogasawara S., Tanaka T., Hozumi Y., Yamaki A., Sakane F, Shirai Y., Nakamura T., Yanaka M., Yamada S., Kaneko M.K., Kato Y., Goto K
    • Journal Title

      Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

      Volume: 37 Issue: 5 Pages: 229-232

    • DOI

      10.1089/mab.2014.0018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Deletion of diacylglycerol kinase e confers susceptibility to obesity via reduced lipolytic activity in murine adipocytes.2018

    • Author(s)
      Nakano T., Seino K., Wakabayashi I., Stafforini D.M., Topham M.K. and Goto K.
    • Journal Title

      FASEB J

      Volume: in press

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] DGKζ ablation engenders upregulation of p53 level in the spleen upon whole-body ionizing radiation2017

    • Author(s)
      Tanaka T., Iseki K., Tanaka K., Nakano T., Iino M. and Goto K.
    • Journal Title

      Advances in Biological Regulation

      Volume: 17 Pages: 30158-30166

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] DGKε欠損マウスにおける適応熱産生機構の解析2022

    • Author(s)
      中野知之、後藤 薫
    • Organizer
      127回日本解剖学会総会
    • Related Report
      2021 Annual Research Report
  • [Presentation] DGKε遺伝子欠損マウスはインスリン依存的に非震え熱産生機機構の障害を引き起こす2021

    • Author(s)
      中野知之、後藤 薫
    • Organizer
      第67回日本解剖学会東北・北海道連合支部学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] DGK epsilon-deficient mice show impaired adaptive thermogenesis under cold exposure2021

    • Author(s)
      Nakano T. and Goto K
    • Organizer
      第126回日本解剖学会総会
    • Related Report
      2020 Research-status Report
  • [Presentation] DGK epsilon deficiency renders mice resistant to LPS-induced inflammatory reaction2021

    • Author(s)
      Ozawa A., Nakano T., Iseki K. and Goto K
    • Organizer
      第126回日本解剖学会総会
    • Related Report
      2020 Research-status Report
  • [Presentation] DGKε欠損マウスにおける体温調節メカニズムの異常2020

    • Author(s)
      中野知之、後藤薫
    • Organizer
      第66回日本解剖学会東北・北海道連合支部学術集会
    • Related Report
      2020 Research-status Report
  • [Presentation] DGKepsilon-deficient visceral adipose tissue is prone to phenotypic switching under high fat diet conditions.2020

    • Author(s)
      Nakano T. and Goto K.
    • Organizer
      Obesity and NAFLD (B3) in Keystone symposia
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ε型ジアシルグリセロールキナーゼ欠損は皮下脂肪における褐色化を促進する2020

    • Author(s)
      中野知之、後藤薫
    • Organizer
      第125回日本解剖学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] ε型ジアシルグリセロールキナーゼ欠損は白色脂肪組織の褐色化を惹起する2019

    • Author(s)
      中野知之、後藤薫
    • Organizer
      第65回日本解剖学会東北・北海道連合支部学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Diacylglycerol kinase epsilon欠損による脂肪組織リモデリングの解析-寒冷暴露による変化-2019

    • Author(s)
      中野知之、後藤 薫
    • Organizer
      第124回日本解剖学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] DGK epsilonはCOX-2を介して脂肪組織のリモデリングを制御する2018

    • Author(s)
      中野知之、後藤 薫
    • Organizer
      第64回日本解剖学会東北・北海道連合支部学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Diacylglycerol kinase alpha negatively regulates the activation of myofibroblasts in mouse liver2018

    • Author(s)
      Nakano T., Seino K., Iseki K. and Goto K.
    • Organizer
      American Society for Cell Biology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] DGKe deficiency impairs lipolytic activity and causes insulin insensitivity in adipocytes under2018

    • Author(s)
      Nakano T. and Goto K.
    • Organizer
      Organ Crosstalk in Obesity and NAFLD (J3) in Keystone symposia
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ジアシルグリセロール代謝不全に起因する脂肪蓄積とインスリンシグナル異常2018

    • Author(s)
      中野知之、後藤 薫
    • Organizer
      第123回日本解剖学会総会シンポジウム
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] DGKε欠損は白色脂肪細胞における脂質代謝異常を惹起する2017

    • Author(s)
      中野知之、後藤 薫
    • Organizer
      第63回日本解剖学会東北・北海道連合支部学術集会
    • Related Report
      2017 Research-status Report
  • [Book] In: Choi S. (eds) Encyclopedia of Signaling Molecules2018

    • Author(s)
      Diacylglycerol Kinase
    • Total Pages
      6
    • Publisher
      Springer
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi