MicroRNA transport from salivary mesenchyme regulates epithelial cytodifferentiation during organogenesis
| Project/Area Number |
17K08520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
Hayashi Toru 北里大学, 医療衛生学部, 講師 (10454266)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 顎下腺 / マイクロRNA / 分化 / 上皮間葉相互作用 / 脱メチル化 / TET / 唾液腺 / エピジェネティクス / 未分化 |
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| Outline of Final Research Achievements |
Epithelial-mesenchymal interactions are required for normal development in organs. We reported that gene expression in epithelium was regulated by microRNAs that are enriched in exosomes from mesenchyme in fetal mouse submandibular gland (SMG). Thus, we hypothesized in this study that “the microRNA signals between tissues” regulate cytodifferentiation during SMG development. Analyses of gene and protein expression using both immunostainings and qPCR suggested that spatio-temporal patterns of enzymes required for cytodifferentiation were regulated in part by the microRNAs imported from the SMG mesenchyme.
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| Academic Significance and Societal Importance of the Research Achievements |
器官発生のメカニズムの全貌は未だ明らかになっていません。異なる組織間での相互作用は、従来タンパク質にだけ着目されてきました。そのような中、私たちは短い核酸（マイクロRNA）が組織間相互作用を仲介することを明らかにしてきました。その研究結果を踏まえ、未熟な器官が機能的に成熟していく過程（分化と言います）に着目したところ、組織間マイクロRNA輸送が分化のタイミングを調節していることが示唆されました。器官再生などの研究分野に新たなアプローチを提供するものです。
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Report
(4 results)
Research Products
(6 results)
