Role of NOX1/NADPH oxidase in acquired immunity

• Project/Area Number: 17K08601
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Matsumoto Misaki 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80533926)
• Co-Investigator(Kenkyū-buntansha): 岩田 和実 京都府立医科大学, 医学(系)研究科(研究院), 講師 (60305571)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 活性酸素種 / 免疫 / 関節炎 / 喘息 / 抗体産生 / 薬理学 / 免疫学

Outline of Final Research Achievements

NOX1 mRNA were expressed in splenic B cells, T cells and CD11b+ monocytes/macrophages. No significant differences were observed in antibody production or in ovalbumin (OVA)-induced asthma between wild-type mice (WT) and Nox1-KO. However, the severity and incidence of experimental collagen-induced arthritis (CIA) following the administration of a low dose of endotoxin (LPS) were significantly lower in Nox1-KO. These results indicate an as yet unidentified role for NOX1 in immune responses.

Academic Significance and Societal Importance of the Research Achievements

免疫応答におけるNOX2 (gp91phox)の重要性はこれまでに詳細に研究されてきたが、免疫応答とNOX1アイソフォームの連関は不明である。本研究の成果は、これまで未知であったNOX1と免疫応答のクロストークを示唆するものであり、関節炎リウマチの発症機序の解明および新しい治療標的の開拓に資するものと思われる。

Research Products

• [Int'l Joint Research] ゲーテ大学(ドイツ)
• [Journal Article] The NOX1 isoform of NADPH oxidase is involved in dysfunction of liver sinusoids in nonalcoholic fatty liver disease. (2018)
  • Author(s): Matsumoto M, Zhang J, Zhang X, Liu J, Jiang JX, Yamaguchi K, Taruno A, Katsuyama M, Iwata K, Ibi M, Cui W, Matsuno K, Marunaka Y, Itoh Y, Torok NJ, Yabe-Nishimura C.
  • Journal Title: Free Radic Biol Med. Volume: 115 Pages: 412-420
  • DOI: 10.1016/j.freeradbiomed.2017.12.019
  • Peer Reviewed / Int'l Joint Research