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Establishment and evaluation of a rapid diagnosis using nanotechnology for amebiasis

Research Project

Project/Area Number 17K08811
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionTokai University

Principal Investigator

TACHIBANA Hiroshi  東海大学, 医学部, 教授 (10147168)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsアメーバ症 / 赤痢アメーバ / 原虫感染症 / 血清診断 / ナノ材料 / 組換えタンパク質 / 感染症 / 診断 / 赤痢アメーバ症
Outline of Final Research Achievements

To establish rapid serodiagnosis for amebiasis, we developed a fluorescence immunochromatography kit using fluorescent silica nanoparticles coated with a recombinant fragment of Entamoeba histolytica-specific surface protein (C-Igl). The kit was evaluated by using sera from patients with amebiasis and other infectious diseases in Japan and Thailand. The study confirmed that the assay has an acceptable level of sensitivity and specificity. In addition, the use of a fluorescence scope was effective for distinguishing between positive and negative results.

Academic Significance and Societal Importance of the Research Achievements

赤痢アメーバ症は、わが国で発生する寄生虫症の中で最も問題になっている疾患の一つである。早期に治療を開始するために確実な診断が必要であり、特に鏡検によって虫体を検出することが難しい腸管外アメーバ症では、血清中の特異抗体の検出が有用である。国内で用いられていたキットが製造中止になっており、本研究で開発された新しい簡便な診断キットの活用が期待できる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (35 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (6 results) Journal Article (14 results) (of which Int'l Joint Research: 11 results,  Peer Reviewed: 14 results,  Open Access: 7 results) Presentation (14 results) (of which Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] チュラロンコン大学(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 復旦大学(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 復旦大学(中国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] チュラロンコン大学(タイ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] チュラロンコン大学(タイ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] 復旦大学(中国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Analysis of D-A locus of tRNA-linked short tandem repeats reveals transmission of Entamoeba histolytica and E. dispar among students in the Thai-Myanmar border region of northwest Thailand2021

    • Author(s)
      Pattanawong U
    • Journal Title

      PLoS Negl Trop Dis

      Volume: 15 Issue: 2 Pages: e0009188-e0009188

    • DOI

      10.1371/journal.pntd.0009188

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand2021

    • Author(s)
      Songsaigath Sunisa、Makiuchi Takashi、Putaporntip Chaturong、Pattanawong Urassaya、Kuamsab Napaporn、Tachibana Hiroshi、Jongwutiwes Somchai
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 3201-3201

    • DOI

      10.1038/s41598-021-82928-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genotyping of Entamoeba nuttalli strains from the wild rhesus macaques of Myanmar and comparison with those from the wild rhesus macaques of Nepal and China2021

    • Author(s)
      Mon Hla Myat、Feng Meng、Pattanawong Urassaya、Kosuwin Rattiporn、Yanagi Tetsuo、Kobayashi Seiki、Putaporntip Chaturong、Jongwutiwes Somchai、Cheng Xunjia、Tachibana Hiroshi
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 92 Pages: 104830-104830

    • DOI

      10.1016/j.meegid.2021.104830

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Autophagy Activated by Peroxiredoxin of Entamoeba histolytica2020

    • Author(s)
      Li Xia、Zhang Yuhan、Zhao Yanqing、Qiao Ke、Feng Meng、Zhou Hang、Tachibana Hiroshi、Cheng Xunjia
    • Journal Title

      Cells

      Volume: 9 Issue: 11 Pages: 2462-2462

    • DOI

      10.3390/cells9112462

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 32020

    • Author(s)
      Wang Zhixin、Wu Duo、Tachibana Hiroshi、Feng Meng、Cheng Xun-jia
    • Journal Title

      Parasites & Vectors

      Volume: 13 Issue: 1 Pages: 592-592

    • DOI

      10.1186/s13071-020-04474-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single-Cell RNA Sequencing Reveals that the Switching of the Transcriptional Profiles of Cysteine-Related Genes Alters the Virulence of Entamoeba histolytica2020

    • Author(s)
      Feng Meng、Zhang Yuhan、Zhou Hang、Li Xia、Fu Yongfeng、Tachibana Hiroshi、Cheng Xunjia
    • Journal Title

      mSystems

      Volume: 5 Issue: 6

    • DOI

      10.1128/msystems.01095-20

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 間接赤血球凝集反応を用いた赤痢アメーバ感染症の血清診断2020

    • Author(s)
      増田剛太、今村顕史、関谷紀貴、前田卓哉、橘 裕司、小林正規
    • Journal Title

      感染症学雑誌

      Volume: 94 Pages: 102-108

    • NAID

      130007886412

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Correlation between genotypes and geographic distribution of Entamoeba nuttalli isolates from wild long-tailed macaques in Central Thailand2019

    • Author(s)
      Feng Meng、Yanagi Tetsuo、Putaporntip Chaturong、Pattanawong Urassaya、Cheng Xunjia、Jongwutiwes Somchai、Tachibana Hiroshi
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 70 Pages: 114-122

    • DOI

      10.1016/j.meegid.2019.02.030

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Whole genome sequencing of Entamoeba nuttalli reveals mammalian host-related molecular signatures and a novel octapeptide-repeat surface protein2019

    • Author(s)
      Masayuki Tanaka, Takashi Makiuchi, Tomoyoshi Komiyama, Takashi Shiina, Ken Osaki, Hiroshi Tachibana
    • Journal Title

      PLOS Neglected Tropical Diseases

      Volume: 13 Issue: 12 Pages: e0007923-e0007923

    • DOI

      10.1371/journal.pntd.0007923

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Influence of Heterologous Transplant of DNA-lacking Mitochondria from Entamoeba histolytica on Proliferation of Entamoeba invadens2018

    • Author(s)
      Kazama Makoto、Yoshida Kazuhiro、Ogiwara Sanae、Makiuchi Takashi、Tachibana Hiroshi
    • Journal Title

      Journal of Eukaryotic Microbiology

      Volume: 0 Issue: 3 Pages: 1-11

    • DOI

      10.1111/jeu.12693

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of a sensitive immunochromatographic kit using fluorescent silica nanoparticles for rapid serodiagnosis of amebiasis2018

    • Author(s)
      Tachibana Hiroshi、Kakino Azumi、Kazama Makoto、Feng Meng、Asai Satomi、Umezawa Kazuo、Nozaki Tsukasa、Makiuchi Takashi、Kamada Takayuki、Watanabe Hideki、Horiki Noriyuki、Cheng Xunjia、Masuda Gohta
    • Journal Title

      Parasitology

      Volume: 145 Issue: 14 Pages: 1890-1895

    • DOI

      10.1017/s0031182018000690

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prevalence and genotypic diversity of Entamoeba species in inhabitants in Kathmandu, Nepal2018

    • Author(s)
      Feng Meng、Pandey Kishor、Yanagi Tetsuo、Wang Ting、Putaporntip Chaturong、Jongwutiwes Somchai、Cheng Xunjia、Sherchand Jeevan B.、Pandey Basu Dev、Tachibana Hiroshi
    • Journal Title

      Parasitology Research

      Volume: 117 Issue: 8 Pages: 2467-2472

    • DOI

      10.1007/s00436-018-5935-2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Naturally acquired IgG antibodies to thrombospondin-related anonymous protein of Plasmodium vivax (PvTRAP) in Thailand predominantly elicit immunological cross-reactivity2018

    • Author(s)
      Kosuwin Rattiporn、Feng Meng、Makiuchi Takashi、Putaporntip Chaturong、Tachibana Hiroshi、Jongwutiwes Somchai
    • Journal Title

      Tropical Medicine & International Health

      Volume: 23 Issue: 8 Pages: 923-933

    • DOI

      10.1111/tmi.13083

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of a sensitive immunochromatographic kit using fluorescent silica nanoparticles for rapid serodiagnosis of amebiasis2018

    • Author(s)
      Tachibana H, Kakino A, Kazama M, Feng M, Asai S, Umezawa K, Nozaki T, Makiuchi T, Kamada T, Watanabe H, Horiki N, Cheng X, Masuda G
    • Journal Title

      Parasitology

      Volume: 143

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ライブイメージングによる赤痢アメーバPeroxiredoxinの動態解析2020

    • Author(s)
      今井達也、牧内貴志 橘 裕司
    • Organizer
      第89回日本寄生虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 赤痢アメーバ症の迅速血清診断のための蛍光イムノクロマト法の評価2019

    • Author(s)
      橘 裕司、Urassaya Pattanawong、垣野あずみ、Napaporn Kuamsab、Chaturong Putaporntip、Somchai Jongwutiwes
    • Organizer
      第60回日本熱帯医学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 赤痢アメーバレクチンIgl1サブユニットの溶血活性領域の同定2019

    • Author(s)
      加藤健太郎、牧内貴志、橘 裕司
    • Organizer
      第88回日本寄生虫学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ライブイメージング法による赤痢アメーバ抗酸化タンパク質の動態解析2019

    • Author(s)
      今井達也、牧内貴志、伊藤 敦、橘 裕司
    • Organizer
      第88回日本寄生虫学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 赤痢アメーバレクチンIglサブユニットの溶血活性領域の同定2018

    • Author(s)
      加藤健太郎、牧内貴志、橘 裕司
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Entamoeba histolytica Iglレクチンの役割2018

    • Author(s)
      加藤健太郎、牧内貴志、程 訓佳、橘 裕司
    • Organizer
      第37回日本糖質学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 赤痢アメーバ症迅速血清診断のための蛍光イムノクロマト法の開発と評価2018

    • Author(s)
      橘 裕司、垣野あずみ、風間 真、馮 萌、浅井さとみ、梅澤和夫、野崎 司、牧内貴志、堀木紀行、程 訓佳、増田剛太
    • Organizer
      第87回日本寄生虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Comparison of hemolytic activity of the intermediate subunit of Entamoeba histolytica and Entamoeba dispar lectins2018

    • Author(s)
      Kentaro Kato、Takashi Makiuchi、Xunjia Cheng、Hiroshi Tachibana
    • Organizer
      第87回日本寄生虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 赤痢アメーバ症の診断 - 知っておきたい近縁種 -2017

    • Author(s)
      橘 裕司
    • Organizer
      第77回日本寄生虫学会東日本支部大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 赤痢アメーバにおけるレクチンサブユニット (Igl) の機能解析2017

    • Author(s)
      牧内貴志、加藤健太郎、Xunjia Cheng、橘 裕司
    • Organizer
      第50回日本原生生物学会・第1回日本共生生物学会合同大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Entamoeba histolyticaとEntamoeba disparのIglレクチンの溶血活性比較研究2017

    • Author(s)
      加藤健太郎、牧内貴志、Xunjia Cheng、橘 裕司
    • Organizer
      第86回日本寄生虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] タイの三日熱マラリア患者におけるPvMSP-9の組換え断片抗原に対する血清抗体の反応性解析2017

    • Author(s)
      Sunisa Songsaigath、牧内貴志、Chaturong Putaporntip、Somchai Jongwutiwes、橘 裕司
    • Organizer
      第86回日本寄生虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Sequence diversity and naturally acquired IgG antibodies against the merozoite surface protein 3 gamma of Plasmodium vivax in Thailand2017

    • Author(s)
      Napaporn Kuamsab、Chaturong Putaporntip、牧内貴志、垣野あずみ、Somchai Jongwutiwes、橘 裕司
    • Organizer
      第86回日本寄生虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Entamoeba レクチンのIglサブユニットが有する溶血活性に関する研究2017

    • Author(s)
      加藤健太郎、牧内貴志、Xunjia Cheng、橘 裕司
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Research-status Report
  • [Book] 赤痢アメーバ感染症.希少感染症のエビデンスと臨床(増田剛太ほか編)2021

    • Author(s)
      増田剛太、錦 信吾、前田卓哉、今村顕史、菅沼明彦、味澤 篤、橘 裕司
    • Publisher
      金芳堂
    • ISBN
      9784765318549
    • Related Report
      2020 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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