| Project/Area Number |
17K08824
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Gifu University |
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Principal Investigator |
Goto Takatsugu 岐阜大学, 糖鎖生命コア研究所, 助教 (80326355)
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| Co-Investigator(Kenkyū-buntansha) |
田中 香お里 岐阜大学, 高等研究院, 教授 (20242729)
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| Project Period (FY) |
2017-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | Bacteroides fragilis / carbapenem 耐性 / 中等度耐性 / 全ゲノム解析 / class D β-lactamase / 薬剤耐性機構 / 薬剤耐性 / カルバペネム耐性 / 全ゲノム配列 / β-lactamase / シャトルベクター / MIC / 嫌気性菌 / カルバペネム耐性菌 / 細菌 / ゲノム / 遺伝子 / 抗生物質 / 薬学 |
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| Outline of Final Research Achievements |
We determined the complete genome sequence of the Bacteroides fragilis GAI92214, a strain intermediately resistant to carbapenems. The GAI92214 chromosome had a unique region, including erythromycin-, tetracycline resistance genes, and an uncharacterized class D β-lactamase gene. Recombinant class D β-lactamase in Escherichia coli significantly degraded ampicillin (64-fold higher MIC than that of the negative control strain) but did not degrade carbapenems. The recombinant enzyme in B. fragilis also did not degrade carbapenems. Other genomic approaches are needed to identify other factors contributed to carbapenem intermediate resistance in the GAI92214 strain.
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| Academic Significance and Societal Importance of the Research Achievements |
ヒト腸管に常在する嫌気性菌 Bacteroides fragilis の耐性株は国内外で多剤耐性傾向にあり、時に化学療法を難渋させる。本耐性株の約 80 % は carbapenem 中等度耐性を呈するが、その耐性機構は世界において未だ解明されていない。我々は本研究にて、世界に先立ち carbapenem 中等度耐性株の全ゲノム配列の解読に成功し、本菌株特有の薬剤耐性遺伝子領域などを見出した点で学術的・社会的意義は高い。本領域中で今回機能解析を行った class D β-lactamase は、当該中等度耐性に寄与していなかったものの、獲得した全ゲノム情報は今後の耐性因子の網羅的探索に役立つ。
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