Analysis of dengue virus replication mechanism using small molecule compounds
| Project/Area Number |
17K08870
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kanagawa Prefectural Institute of Public Health (2018-2020)
Tokyo Metropolitan Institute of Medical Science (2017) |
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Principal Investigator |
Hishiki Takayuki 神奈川県衛生研究所, 微生物部, 主任研究員 (30535761)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | デングウイルス / ジカウイルス / 抗ウイルス化合物 / ウイルス複製機構 / ケミカルバイオロジー / 複製機構 |
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| Outline of Final Research Achievements |
Dengue virus (DENV), transmitted to humans by Aedes mosquitoes, is the aetiological agent of dengue fever and dengue haemorrhagic fever and continues to be a public health problem in the tropical and subtropical areas. However, there is currently no antiviral treatment for DENV infection. In this study, our aim was to analyze the DENV replication mechanism and obtain basic information leading to the development of new antiviral agents. As a result of analysis using infectious DENV and replicon cells, we identified some compounds that have potent anti-DENV activity and low cytotoxicity.
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| Academic Significance and Societal Importance of the Research Achievements |
抗ウイルス化合物の同定と分子作用機序の解明は、デングウイルス生活環の新たな側面を明らかにするだけでなく、他のウイルス複製機構の解明、さらには宿主細胞の理解への波及効果をもたらす事が予想される。またそれらの知見は、新たな創薬標的分子を見いだすことに繋がり、より効率的な抗ウイルス剤開発への応用が期待される。さらに今回同定した抗ウイルス化合物のなかには承認薬も含まれている事から、ドラッグリポジショニングによる新薬開発に繋がる可能性がある。
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Report
(5 results)
Research Products
(17 results)
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[Journal Article] Sphingomyelin Is Essential for the Structure and Function of the Double-Membrane Vesicles in Hepatitis C Virus RNA Replication Factories2020
Author(s)
Gewaid H, Aoyagi H, Arita M, Watashi K, Suzuki R, Sakai S, Kumagai K, Yamaji T, Fukasawa M, Kato F, Hishiki T, Mimata A, Sakamaki Y, Ichinose S, Hanada K, Muramatsu M, Wakita T, Aizaki H.
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Journal Title
J Virol
Volume: 94
Issue: 23
DOI
Related Report
Peer Reviewed
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[Journal Article] Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses2019
Author(s)
Yamane D, Feng H, Rivera-Serrano EE, Selitsky SR, Hirai-Yuki A, Das A, McKnight KL, Misumi I, Hensley L, Lovell W, González-López O, Suzuki R, Matsuda M, Nakanishi H, Ohto-Nakanishi T, Hishiki T, Wauthier E, Oikawa T, Morita K, Reid LM, Sethupathy P, Kohara M, Whitmire JK, Lemon SM
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Journal Title
Nature Microbiology
Volume: 印刷中
Issue: 7
Pages: 1096-1104
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Stearoyl-CoA desaturase-1 is required for flavivirus RNA replication2019
Author(s)
Hishiki Takayuki、Kato Fumihiro、Nio Yasunori、Watanabe Satoru、Wen Tan Nicole Wei、Yamane Daisuke、Miyazaki Yasuyuki、Lin Chun-Chieh、Suzuki Rieko、Tajima Shigeru、Lim Chang-Kweng、Saijo Masayuki、Hijikata Makoto、Vasudevan Subhash G.、Takasaki Tomohiko
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Journal Title
Antiviral Research
Volume: 165
Pages: 42-46
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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