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Risk assessment for parasitic food poisoning caused by Sarcocystis species in sika deer

Research Project

Project/Area Number 17K09185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hygiene and public health
Research InstitutionOsaka Institute of Public Health

Principal Investigator

Abe Niichiro  地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 総括研究員 (10321936)

Co-Investigator(Kenkyū-buntansha) 高島 康弘  岐阜大学, 応用生物科学部, 准教授 (20333552)
森部 絢嗣  岐阜大学, 応用生物科学部, 准教授 (50456620)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords寄生虫 / 寄生原虫 / 住肉胞子虫 / シカ / ジビエ / 食品衛生 / 食中毒
Outline of Final Research Achievements

In Japan, the classification of Sarcocystis species found in sika deer has been unclear. The present study has provided molecular and morphological evidence for the presence of seven Sarcocystis spp., including three new species, in sika deer in Japan, that is, S. japonica n. sp., S. cf. tarandi, S. matsuoae n. sp., S. pilosa, S. gjerdei n. sp., S.cf. taeniata and S. ovalis. Multiplex PCR and real-time PCR assays capable of identifying the seven species found in this study has been developed and might become useful in future studies aimed at identifying the causative species in Sarcocystis related food poisoning or the definitive hosts of these species.

Academic Significance and Societal Importance of the Research Achievements

わが国の野生シカに寄生する住肉胞子虫の分類は、形態類似種との鑑別が困難なため不明であった。本研究ではシカ寄生種の分類に遺伝学的手法を導入することで新種の存在を示し、これまで混沌としたわが国のその分類に明確な方向性をもたらした。近年、ジビエ嗜好の高まりとともに、不適切な調理による健康被害が発生している。シカ肉に寄生する住肉胞子虫が原因とされているが寄生種の同定には至らず、効果的な検査法も確立されていなかった。本研究成果である住肉胞子虫の分類及びマルチプレックスPCRやリアルタイムPCRによる寄生種の同定法は、食中毒事例での原因究明や固有宿主の探索に大きく貢献すると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] Molecular differentiation of five Sarcocystis species in sika deer (Cervus nippon centralis) in Japan based on mitochondrial cytochrome c oxidase subunit I gene (coxI)2019

    • Author(s)
      Niichiro Abe, Kayoko Matsuo, Junji Moribe, Yasuhiro Takashima, Takashi Baba, Bjorn Gjerde
    • Journal Title

      Parasitology Research

      Volume: 118 Issue: 6 Pages: 1975-1979

    • DOI

      10.1007/s00436-019-06319-5

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Morphological and molecular characteristics of seven Sarcocystis species from sika deer (Cervus nippon centralis) in Japan, including three new specis2019

    • Author(s)
      Niichiro Abe, Kayoko Matsuo, Junji Moribe, Yasuhiro Takashima, Takao Irie, Takashi Baba, Bjorn Gjerde
    • Journal Title

      International Journal for Parasitology: Parasites and Wildlife

      Volume: 10 Pages: 252-262

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 岐阜県産野生ホンシュウジカに寄生する住肉胞子虫の遺伝子解析2019

    • Author(s)
      阿部仁一郎,森部絢嗣,高島康弘,松尾加代子,入江隆夫,馬場孝
    • Organizer
      第88回日本寄生虫学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 岐阜県産ホンシュウジカに寄生する住肉胞子虫の形態と遺伝子解析2018

    • Author(s)
      阿部仁一郎,森部絢嗣,高島康弘,松尾加代子,馬場孝
    • Organizer
      第161回日本獣医学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 岐阜県産ホンシュウジカとイノシシから検出された住肉胞子虫の遺伝子解析2017

    • Author(s)
      阿部仁一郎、松尾加代子、森部絢嗣、高島康弘
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 岐阜県産ホンシュウジカとイノシシから検出された住肉胞子虫の遺伝子解析2017

    • Author(s)
      阿部仁一郎、松尾加代子、森部絢嗣、高島康弘
    • Organizer
      第73回日本寄生虫学会西日本支部大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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