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High risk markers for cancer in Barrett's esophagus

Research Project

Project/Area Number 17K09364
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionKawasaki Medical School

Principal Investigator

Takahisa Murao  川崎医科大学, 医学部, 講師 (40388971)

Co-Investigator(Kenkyū-buntansha) 塩谷 昭子  川崎医科大学, 医学部, 教授 (80275354)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsバレット食道腺癌 / PDZK1 / バレット腺癌 / LSBE / SSBE / SERPINB7
Outline of Final Research Achievements

In a previous study, we found that PDZK1 expression is higher in long segment BE compared to that in short-segment BE. Human adenocarcinoma-derived OE33 cells were used as a parental cell line and transfected to generate PDZK1 overexpressed OE33 cells (PC cells) or transfected with empty vector as control cells (NC cells).
There were no significant differences in cell growth between NC and PC cells. PSI significantly increased apoptosis in NC cells, but not in PC cells. In response to PSI, increased levels of cleaved-caspase3 and decreased pro-caspase3 levels were found in NC cells, but not in PC cells. In NC cells, PSI significantly decreased Bcl-2 expression without affecting Bax levels. In contrast, high expression of both Bcl-2 and Bax was observed in PC cells.
Overexpression of PDZK1 protein induces an apoptosis-resistant phenotype in BE cells, which may be a potential therapeutic target.

Academic Significance and Societal Importance of the Research Achievements

近年、下部食道のバレット上皮から発生する食道腺癌は急激な増加を示し、海外において最も増加率が高い癌である。
我々は米国人と日本人の検体を用いて癌の合併率が高いバレット上皮においてPDZK1の発現が高いことを調べてきた。今回、PDZK1の役割を明らかにする目的で食道腺癌細胞株にPDZK1を過剰発現させた細胞を作成した。対照の細胞と比較すると細胞の増殖速度に差は認めなかったが、アポトーシス(細胞が構成している組織をより良い状態に保つため、細胞自体に組み込まれた細胞の自然死)刺激を行うと、アポトーシスの増加抑制を認めた。そのためPDZK1は食道腺癌において癌の抵抗性を増加させる可能性が考えられた。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2018 2017 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] PDZK1 induces resistance to apoptosis in esophageal adenocarcinoma cells2021

    • Author(s)
      Handa Osamu、Goda Kyousuke、Handa Yukiko、Fukushima Shinya、Osawa Motoyasu、Murao Takahisa、Matsumoto Hiroshi、Umegaki Eiji、Fujita Yoshihiko、Nishio Kazuto、Shiotani Akiko
    • Journal Title

      Esophagus

      Volume: - Issue: 3 Pages: 655-662

    • DOI

      10.1007/s10388-021-00819-z

    • NAID

      40022594543

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular biomarker identification for esophageal adenocarcinoma using endoscopic brushing and magnified endoscopy2020

    • Author(s)
      Goda K, Murao T, Handa Y, Katsumata R, Fukushima S, Nakato R, Osawa M, Ishii M, Fujita M, Handa O, Matsumoto H, Fujita Y, Nishio K, Wallace TM, Gomez-Esquivel R, Berzosa M, Wolfsen HC, Wallace MB, Umegaki E, Shiotani A
    • Journal Title

      Esophagus

      Volume: 18 Issue: 2 Pages: 306-314

    • DOI

      10.1007/s10388-020-00762-5

    • NAID

      40022523276

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] バレット食道のバレット腺癌リスクマーカーPDZK1 の抗アポトーシス能増強作用2020

    • Author(s)
      半田 修,半田有紀子,合田杏佑,福嶋真弥,平松和恵,萩原喜美子,村尾高久,藤田至彦,塩谷昭子
    • Organizer
      第38回Cytoprotection研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] バレット食道のバレット腺癌リスクマーカーPDZK1 の抗アポトーシス能増強作用2020

    • Author(s)
      半田修,半田有紀子,合田杏佑,福嶋真弥,平松和恵,萩原喜美子,村尾高久,藤田至彦,塩谷昭子
    • Organizer
      第38回Cytoprotection研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 内視鏡下ブラッシング法を用いたバレット食道に関連する分子バイオマーカーについての検討2018

    • Author(s)
      合田 杏佑
    • Organizer
      第50回胃病態機能研究会 主題1-2 Young Investigator Session『酸関連疾患をめぐる諸問題』
    • Related Report
      2018 Research-status Report
  • [Presentation] Molecular biomarkers of Barrett’s esophagus using endoscopic brushing samples2017

    • Author(s)
      Kyosuke Goda, Takahisa Murao, Akiko Shiotani
    • Organizer
      第93回日本消化器内視鏡学会総会ワークショップ
    • Related Report
      2017 Research-status Report
  • [Remarks] 川崎医科大学 消化管内科学教室H.P.

    • URL

      https://m.kawasaki-m.ac.jp/classroom/course.php?id=404

    • Related Report
      2020 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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