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Synergistic Cooperation of Heterogeneous Stromal Cells for Wound Repair Priming

Research Project

Project/Area Number 17K09559
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cardiovascular medicine
Research InstitutionDokkyo Medical University

Principal Investigator

Kishimoto Satoko  獨協医科大学, 医学部, 講師 (10511488)

Co-Investigator(Kenkyū-buntansha) 井上 健一  獨協医科大学, 医学部, 准教授 (90587974)
佐久間 理吏  獨協医科大学, 医学部, 准教授 (10530199)
井上 晃男  獨協医科大学, 医学部, 教授 (20168454)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords創傷治癒 / 間質細胞 / 傷害刺激 / 血管新生 / 脂肪由来間質細胞
Outline of Final Research Achievements

We show that surgical treatments for adipose tissue reproducibly increase and reorganize adipose-derived stromal vascular fractions (SVFs). We term this phenomenon “wound repair priming” and subsequently developed a method to reproduce this phenomenon and investigated its mechanisms. The primed SVFs required two surgical procedures: mincing fat parenchyma (injury) and ligating the subcutaneous fat-feeding artery (ischemia). The primed SVFs rapidly reorganize their cell components during wound repair. Residential stromal cells and mobilized immune cells collaborate to achieve effective angiogenesis in ischemic tissues.

Academic Significance and Societal Importance of the Research Achievements

我々は、異種雑多な間質細胞が創傷治癒というゴールを目指してチームとして機能するという新たな概念(創傷治癒プライミング)を提唱し、そのメカニズムについて研究することに学術的な意義があると考える。
臨床現場で移植細胞の品質を均一に確保することが難しいという問題を解決するには、将来的に、外科的なプライミングと同等の効果が得られるアプローチ、例えば自然免疫シグナルを刺激する化学物質を用いたプライミングなどが有望な代替手段となり得る。このような化学的・薬理学的なプライミングは、重症虚血肢などの虚血性心血管疾患に対する効果的な血管新生療法の開発に貢献すると考えられる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (10 results)

All 2020 2019 2018 2017

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (2 results)

  • [Journal Article] Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model2020

    • Author(s)
      Kishimoto S, Inoue KI, Sohma R, Toyoda S, Sakuma M, Inoue T, Yoshida K
    • Journal Title

      Stem Cells International

      Volume: 7219149 Pages: 7219149-7219149

    • DOI

      10.1155/2020/7219149

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increases Angiogenic Capacity in a Mouse Limb Ischemia Model2019

    • Author(s)
      Kishimoto S, Inoue KI, Sohma R, Toyoda S, Sakuma M, Inoue T, Yoshida KI
    • Journal Title

      Stem Cells International

      Volume: in press

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cancer-associated fibroblasts show heterogeneous gene expression and induce vascular endothelial growth factor A (VEGFA) in response to environmental stimuli2019

    • Author(s)
      Inoue KI, Kishimoto S, Akimoto K, Sakuma M, Toyoda S, Inoue T, Yoshida KI, Shimoda M, Suzuki S
    • Journal Title

      Ann Gastroenterol Surg

      Volume: 3 Issue: 4 Pages: 416-425

    • DOI

      10.1002/ags3.12249

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Polyelectrolyte Complexes of natural polymers and their biomedical applications2019

    • Author(s)
      Ishihara M, Kishimoto S, Nakamura S, Sato Y, Hattori H
    • Journal Title

      Polymers (Basel)

      Volume: 11 Issue: 4 Pages: 672-672

    • DOI

      10.3390/polym11040672

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Low-Molecular Weight Heparin/Protamine Micro-Nanoparticles Augmented Viability of Human Adipose-Derived Regenerative Cells.2018

    • Author(s)
      Masashi Sakuma, Satoko Kishimoto, Ken-ichi Inoue, Ryoichi Sohma, Shigeru Toyoda, Hideki Iwaguro and Teruo Inoue
    • Journal Title

      Biomedical Journal of Scientific and Technical Research

      Volume: 12 Issue: 3 Pages: 1-3

    • DOI

      10.26717/bjstr.2018.12.002265

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autologous and heterotopic transplantation of adipose stromal vascular fraction ameliorates stress urinary incontinence in rats with simulated childbirth trauma2018

    • Author(s)
      Ken-ichi Inoue, Satoko Kishimoto, Kanya Kaga, Miki Fuse, Akira Furuta, Tomonori Yamanishi
    • Journal Title

      Regenerative Therapy

      Volume: 8 Pages: 9-14

    • DOI

      10.1016/j.reth.2017.11.003

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reproducible insulin secretion from isolated rat pancreas preparations using an organ bath2018

    • Author(s)
      Asuka Morita, Motoshi Ouchi, Misao Terada, Hiroe Kon, Satoko Kishimoto, Keitaro Satoh, Naoyuki Otani, Keitaro Hayashi, Tomoe Fujita, Ken-ichi Inoue, Naohiko Anzai
    • Journal Title

      Experimental Animals

      Volume: 67 Issue: 1 Pages: 15-22

    • DOI

      10.1538/expanim.17-0059

    • NAID

      130006339985

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biomaterials as cell carriers for augmentation of adipose tissue-derived stromal cell transplantation.2018

    • Author(s)
      Masayuki Ishihara, Satoko Kishimoto, Shingo Nakamura, Koichi Fukuda, Yoko Sato, Hidemi Hattori
    • Journal Title

      Bio-Medical Materials and Engineering

      Volume: 29 Issue: 5 Pages: 567-585

    • DOI

      10.3233/bme-181009

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 組織間質細胞の修復戦略を細胞社会の文脈で理解する2018

    • Author(s)
      井上健一, 岸本聡子, 井上晃男, 吉田謙一郎
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2018 Research-status Report 2017 Research-status Report
  • [Presentation] 創傷治癒における間質細胞の傷害刺激感知と血管新生増強2017

    • Author(s)
      岸本聡子, 井上健一, 朝戸裕貴, 井上晃男, 吉田謙一郎
    • Organizer
      第47回日本創傷治癒学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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