Establishment of biomarkers for skeletal muscle regeneration process and examination of its clinical efficacy

• Project/Area Number: 17K09807
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Wakayama Medical University
• Principal Investigator: Murata Ken-ya 和歌山県立医科大学, 医学部, 教授 (90264853)
• Co-Investigator(Kenkyū-buntansha): 伊東 秀文 和歌山県立医科大学, 医学部, 教授 (20250061) ; 檜皮谷 泰寛 和歌山県立医科大学, 医学部, 博士研究員 (40405452) ; 森 めぐみ 和歌山県立医科大学, その他部局等, 助教 (60590437) ; 廣西 昌也 和歌山県立医科大学, 医学部, 教授 (80316116)
• Project Period (FY): 2017-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 骨格筋再生 / 筋再生因子 / 筋線維分化 / TRPV1 / 筋壊死 / 筋再生 / 内科

Outline of Final Research Achievements

Promotion of skeletal muscle regeneration is important from the viewpoint of treating muscle diseases and preventing disuse muscle atrophy in the elderly. We have reported that calpain and urokinase play an important role in skeletal muscle regeneration. In this study, we focused on TRPV1, one of the transient receptor potential (TRP) channel families. TRPV1 is reported to be involved in the transmission of sensory stimuli, such as pain stimuli, and this time, by applying myoblast proliferation, we aimed to develop a biomarker that predicts muscle regeneration. Furthermore, we established a rehabilitation training method to prevent muscle atrophy and contributed to the prevention of sarcopenia, which is a problem in an aging society.

Academic Significance and Societal Importance of the Research Achievements

申請者らは、Transient receptor potential(TRP)チャンネルファミリーのひとつのTRPV1に注目し、各種筋疾患や筋損傷モデルマウスの生検筋を用いて病理学的、生化学的、分子生物学的に手法にてTRPV1のバイオマーカーをとしての有用性を検討した。そして、筋萎縮モデルにTPPV1アゴニストを投与し、筋萎縮予防効果を検討した。骨格筋再生のバイオマーカーの探索は、筋疾患や廃用による筋萎縮を予防し、筋再生を促す治療法を確立することにある。

Research Products

• [Journal Article] Anti-ganglionic AChR antibodies in Japanese patients with motility disorders (2018)
  • Author(s): Mukaino A, Minami H, Isomoto H, Hamamoto H, Ihara E, Maeda Y, Higuchi O, Okanishi T, Kokudo Y, Deguchi K, Sasaki F, Ueki T, Murata KY, Yoshida T, Kinjo M, Ogawa Y, Ido A, Matsuo H, Nakao K, Nakane S
  • Journal Title: J Gastroenterol Volume: 53 Issue: 12 Pages: 1227-1240
  • DOI: 10.1007/s00535-018-1477-8
  • Peer Reviewed / Open Access
• [Journal Article] Repeated hyperhidrosis and chilblain-like swelling with ulceration of the fingers and toes in hereditary sensory and autonomic neuropathy type II. (2018)
  • Author(s): Shima T, Yamamoto Y, Kanazawa N, Murata KY, Ito H, Kondo T, Yuan J, Hashiguchi A, Takashima H, Furukawa F
  • Journal Title: J Dermatol Volume: 0 Issue: 11 Pages: 0-0
  • DOI: 10.1111/1346-8138.14336
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Superficial siderosis associated with duplicated dura mater detected by CISS reverse MRI (2018)
  • Author(s): Hosokawa M, Murata K, Horonishi M, Koh J, Nishioka K, Nakao N, Ito H
  • Journal Title: Journal of neurological science Volume: 392 Pages: 38-43
  • DOI: 10.1016/j.jns.2018.07.001
  • Peer Reviewed
• [Journal Article] Nivolumab causes focal muscular inflammation without electrophysiological or muscle magnetic resonance imaging findings (2018)
  • Author(s): Li F, Ayaki T, Sakiho Ueda, Nagai H, Murata K, and Takahashi R
  • Journal Title: Neurology and clinical neuroscience Volume: 6 Issue: 4 Pages: 112-114
  • DOI: 10.1111/ncn3.12193
  • Peer Reviewed / Open Access