| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The causative gene MEN1 of multiple endocrine neoplasia type 1(MEN1) is considered to be a tumor suppressor gene, but the function of menin, a translation product of MEN1, in non-endocrine tissues has not been fully elucidated. The function of menin in skeletal muscle was analyzed. A cDNA microarray analysis using skeletal muscle of Men1 heterozygous mice revealed that the variation of glucose metabolism-related genes was larger than that of the wild type. We also confirmed that the expression level of menin fluctuates during the process of differentiation of C2C12 myoblast cells into myotubes. Immunoblotting analysis using the extracted protein of isolated skeletal muscle after insulin administration revealed that the deletion of menin affected the expression levels of multiple proteins related to glucose metabolism.
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