| Project/Area Number |
17K10003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
横大路 智治 広島大学, 医系科学研究科(薬), 准教授 (70389120)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 食物アレルギー / エビ / 抗原 / FDEIA / アレルゲン / 食物依存性運動誘発アナフィラキシー |
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| Outline of Final Research Achievements |
Sensitivity of the present inspection method for diagnosis of food-dependent exercise-induced anaphylaxis (FDEIA) caused by shrimp is insufficient. In this study, we identified causative allergens and tried to development accurate method to diagnose the shrimp FDEIA.
Soluble proteins from 4 species of shrimp (L. vannamei, P. monodon, F chinensis and M japonicus) were extracted and IgE-binding proteins were searched. As the result, we identified fructose 1,6-bisphosphate aldolase (FBA) having 40 kDa molecular weight and Nesprin-1 homolog having 70 kDa as allergens for shrimp FDEIA. It was also revealed that 9.5% and 19% patients had specific IgE antibodies to FBA and Nesprin-1, respectively.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、エビ摂取と運動負荷によってエビFDEIAと確定診断された患者血清を用いて抗原解析を実施し、通常の即時型エビアレルギーの原因抗原であるトロポミオシン、筋形質カルシウム結合タンパク質、アルギニンキナーゼ、トロポニンC、ミオシン軽鎖、ヘモシアニンとは異なるFDEIAに特異的な新規抗原を明らかにした。今後、これらの抗原を組み合わせることで精度の高いin vitro 診断法が確立されると、患者にとって危険な負荷試験の実施を回避できる。さらに、同定した抗原を用いたエビFDEIAの予防や治療法の確立が期待される。
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