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Development of a novel pneumococcal vaccine with iron oxide nanoparticle for mucosal immunity

Research Project

Project/Area Number 17K10013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Infectious disease medicine
Research InstitutionTohoku University

Principal Investigator

Ishii Keiko  東北大学, 医学系研究科, 准教授 (00291253)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords新規肺炎球菌ワクチン / ユニバーサルワクチン / 酸化鉄ナノ粒子 / アジュバント / 感染防御効果 / 肺炎球菌 / ナノ粒子ワクチン / 粘膜免疫 / 粘膜免役
Outline of Final Research Achievements

We constructed a novel pneumococcal vaccine, PspA-IONP, consisted of pneumococcal surface protein A (PspA) and iron oxide nanoparticle (IONP) with a novel technique. Intratracheal immunization of mice with PspA-IONP induced high levels of systemic PspA-specific IgG antibody than with PspA alone and mucosal PspA-specific IgA antibody that was not detected with PspA alone. When the PspA-IONP-immunized mice were infected with Streptococcus pneumoniae, bacterial proliferation was suppressed, and survival was markedly improved. These results suggest the availability of PspA-IONP as a mucosal immunity-inducing pneumococcal vaccine.

Academic Significance and Societal Importance of the Research Achievements

現行の肺炎球菌ワクチンは一部の血清型の菌に有効であるが、ワクチンに含まれない血清型の菌が増加する「血清型置換」の解決が課題となっている。本研究では、すべての血清型の菌に存在するpneumococcal surface protein A(PspA)を酸化鉄ナノ粒子(iron oxide nanoparticle: IONP)に独自の方法で結合させたPspA-IONP を作成し、マウスでその効果を実証した。IONPは吸入用ドライパウダー製剤化を目指したアジュバントであり、他のワクチンにも応用可能である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 Other

All Presentation (3 results) Remarks (1 results)

  • [Presentation] 新規肺炎球菌ナノ粒子ワクチンによる抗体産生と感染防御効果2019

    • Author(s)
      中平絢子、岩岡大貴、佐藤光、笠松純、石井恵子、川上和義
    • Organizer
      第30回日本生体防御学会学術総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 酸化鉄ナノ粒子を用いた新規肺炎球菌ワクチンの開発2019

    • Author(s)
      中平絢子、岩岡大貴、石井恵子、明田幸宏、川上和義
    • Organizer
      第68回日本感染症学会東日本地方会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Antibody production and host protection from pneumococcal infection by a novel nanoparticle vaccine2019

    • Author(s)
      Ayako Nakahira, Hiroki Iwaoka, Ko Sato, Jun Kasamatsu, Keiko Ishii, Kazuyoshi Kawakami
    • Organizer
      第48回日本免疫学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 東北大学大学院医学系研究科保健学専攻基礎検査医科学領域 感染分子病態解析学分野

    • URL

      http://www.infect-immun.med.tohoku.ac.jp/

    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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