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Improvement in making methods of PUVA-treated dendritic cells

Research Project

Project/Area Number 17K10135
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatrics
Research InstitutionKanazawa University (2018-2019)
Department of Clinical Research, National Hospital Organization Kanazawa Medical Center (2017)

Principal Investigator

HIDEAKI MAEBA  金沢大学, 附属病院, 特任助教 (10419335)

Co-Investigator(Kenkyū-buntansha) 西村 良成  金沢大学, 附属病院, 講師 (50324116)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
KeywordsGVHD / psoralen / UVA / 制御性樹状細胞
Outline of Final Research Achievements

We have reported that bone marrow-derived dendritic cells (BM-DCs), which are generated from bone marrow cells plus GM-CSF, acquired tolerogenicity by PUVA-treatment(psoralen+UVA 2J/cm2) in mice.The PUVA-treated DCs have tolerogenic function in a MHC-independent manner. But these cells did not have the inhibitory effects in the mouse GVHD model. We tried to improve the manufacture condition of PUVA-DC, to make these cells engraft effectively in vivo. At first, we researched the setting (0-2 J /cm2) of the UVA radiation, but did not have acquired tolerance in the UVA 0.2-1J/cm2 setting. Next, we added caspase inhibitor to PUVA treatment for apoptotic suppression by the UVA radiation. The survival rate 24 hours after PUVA-treatment were not improved, and these cells did not have the acquired more tolerance than conventional PUVA-DC. The examination of the further culture condition is necessary to use PUVA-DC for cell therapy.

Academic Significance and Societal Importance of the Research Achievements

造血幹細胞移植の合併症であるGVHDはHLA適合や免疫抑制剤の開発がなされてきた現在においてもII度以上のGVHDは非血縁者間で40%以上発症し、原疾患の治癒が得られても死亡する症例が存在する。
我々は、マウス骨髄細胞からpsolarenと紫外線(UVA)を用いて、制御性樹状細胞(PUVA-DC)を開発し、その細胞はマウスモデルでMHC非依存性にTリンパ球に対し抑制性の作用を示した。この技術を人に応用し、HLAの壁を乗り越える安全な骨髄移植開発のため、PUVA-DCの至適作製条件の検討を行った.

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (7 results)

  • [Journal Article] Accurate detection of renal leukemic involvement in children using 3-Dcomputed tomography modeling2019

    • Author(s)
      Toshihiro F, Ryosei N, Shintaro M, Rie K, Yasuhiro I, Raita A, Hideaki M, Akihiro Y
    • Journal Title

      Pediatrics International

      Volume: 61 Issue: 7 Pages: 679-687

    • DOI

      10.1111/ped.13907

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nonallergic cutaneous pigmentation is commonly observed after methotrexate administration2019

    • Author(s)
      Yasuhiro I, Ryosei N, Raita A, Kazuhiro N, Masaki F, Toshihiro F, Rie K,Shintaro M, ,Hideaki M, Akihiro Y
    • Journal Title

      J Oncol Pharm Pract

      Volume: 25 Issue: 3 Pages: 769-771

    • DOI

      10.1177/1078155218815557

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deep spontaneous molecular remission in a patient with congenital acute myeloid leukemia expressing a novel MOZ-p300 fusion transcript.2018

    • Author(s)
      Ikawa Y, Nishimura R, Maeba H, Fujiki T, Kuroda R, Noguchi K, Fukuda M, Mase S, Araki R, Mitani Y, Sato T, Terui K, Ito E, Kitabayashi I, Yachie A.
    • Journal Title

      Leuk Lymphoma

      Volume: 印刷中 Issue: 10 Pages: 1-3

    • DOI

      10.1080/10428194.2018.1434885

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A characteristic flow cytometric pattern with broad forward scatter and narrowed side scatter helps diagnose immune thrombocytopenia (ITP)2018

    • Author(s)
      Raita Araki,Ryosei Nishimura,Rie Kuroda,Toshihiro Fujiki,Shintaro Mase,Kazuhiro Noguchi,Yasuhiro Ikawa,Hideaki Maeba,Akihiro Yachie
    • Journal Title

      International Journal of Hematology

      Volume: 108 Issue: 2 Pages: 151-160

    • DOI

      10.1007/s12185-018-2454-y

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hearing impairment accompanied with low-tone tinnitus during all trans retinoic acid containing chemotherapy2017

    • Author(s)
      Fujiki Toshihiro、Nishimura Ryosei、Ikawa Yasuhiro、Noguchi Kazuhiro、Mase Shintaro、Kuroda Rie、Araki Raita、Maeba Hideaki、Yachie Akihiro
    • Journal Title

      Pediatric Blood & Cancer

      Volume: 65 Issue: 3

    • DOI

      10.1002/pbc.26864

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 難治性リンパ系腫瘍に対する長期維持療法としての少量クロファラビン併用化学療法2019

    • Author(s)
      前馬 秀昭, 野口 和寛, 福田 正基, 黒田 梨絵, 藤木 俊寛, 馬瀬 新太郎, 荒木 来太, 伊川 泰広
    • Organizer
      日本血液学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 造血幹細胞移植患者における高頻度に認めたMRワクチンに対する抗体産生不全2019

    • Author(s)
      前馬 秀昭, 野口 和寛, 福田 正基, 黒田 梨絵, 藤木 俊寛, 馬瀬 新太郎, 荒木 来太, 伊川 泰広, 和田 泰三
    • Organizer
      第42回日本造血細胞移植学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 縦隔リンパ管奇形を合併した多発限局性結節性過形成症候群の12歳女児例2019

    • Author(s)
      前馬 秀昭, 酒井 清祥
    • Organizer
      日本血管腫血管奇形学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 縦隔リンパ管奇形を合併した多発限局性結節性過形成症候群-12歳女児例-2019

    • Author(s)
      前馬 秀昭, 酒井 清祥, 野口 和寛, 福田 正基, 黒田 梨絵, 藤木 俊寛, 馬瀬 新太郎, 荒木 来太, 伊川 泰広, 和田 泰三
    • Organizer
      第61回日本小児血液・がん学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 神経芽腫治療終了後、早期に肝臓に発症した限局性結節性過形成の2例2018

    • Author(s)
      前馬 秀昭、野口 和寛、福田 正基、藤木 俊寛、黒田 梨絵、馬瀬新太郎、 荒木 来太、伊川 泰広、谷内江昭宏
    • Organizer
      第60回日本小児血液・がん学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] ダウン症候群における急性リンパ性白血病に対する骨髄非破壊的移植施行した2症例の検討2018

    • Author(s)
      前馬 秀昭、西村 良成、野口 和寛、福田 正基、藤木 俊寛、黒田 梨絵、馬瀬新太郎、 荒木 来太、伊川 泰広、谷内江昭宏
    • Organizer
      第40回造血幹細胞移植学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] HPV-B19感染後に生じた一過性後天性von Willebrand症候群2017

    • Author(s)
      前馬 秀昭、西村 良成、野口 和寛、福田 正基、藤木 俊寛、黒田 梨絵、馬瀬新太郎、 荒木 来太、伊川 泰広、谷内江昭宏
    • Organizer
      第59回 日本小児血液・がん学会学術集会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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